DOI QR코드

DOI QR Code

Viability Assay of Seaweeds Responding to Mountain Fire-Related Pollutants

산불 관련 해양환경오염원들에 대한 해조류의 활력 측정

  • KANG Se-Eun (Department of Biotechnology, Pukyong National University) ;
  • JIN Long-Guo (Department of Biotechnology, Pukyong National University) ;
  • CHOI Jae-Suk (Department of Biotechnology, Pukyong National University) ;
  • CHO Ji-Young (Department of Biotechnology, Pukyong National University) ;
  • SHIN Hyun-Woung (Department of Marine Biotechnology, Soonchunhyang University) ;
  • HONG Yong-Ki (Department of Biotechnology, Pukyong National University)
  • 강세은 (부경대학교 생물공학과) ;
  • ;
  • 최재석 (부경대학교 생물공학과) ;
  • 조지영 (부경대학교 생물공학과) ;
  • 신현웅 (순천향대학교 해양생명공학과) ;
  • 홍용기 (부경대학교 생물공학과)
  • Published : 2003.06.01

Abstract

Plant ash and soil drainage, derived by frequent mountain fires during winter, might cause biological contamination to seaweeds at seashore and river mouse area. To thalli of Ulva pertusa, maximum non-lethal concentration(MNLC), lethal concentration 50 $(LC_{50})$ and minimum lethal concentration (MLC) of pine needle ash were shown as 60, 350 and 550 mg/mL, respectively. The yellow loess and granite sand did not damage at concentrations of 20 and 200 mg/mL, respectively To thalli of Porphyra yezoensis, the MNLC, LC5O, MLC of pine needle ash were shown as 0.08, 0.4 and 1.0 mg/mL, respectively. Effects of yellow loess and granite sand were approximately 1/2 and 1/10 of the ash. To thalli of Undaria pinnatifida, the pine needle ash, yellow loess and granite sand did not damage at the concentration range of 20 to 40 mg/mL. Change of pigments $(chlorophyll\;\alpha,\;lutein,\;\beta-carotene,\;phycoerthrin)$ was also determined at the MNLC, $LC-{50}$ and MLC of pine needle ash. Among three seaweeds tested, P. yezoensis produced the most 2.7-fold of lutein and 2.3-fold of $\beta-carotene$ at $LC-{50}$ of the ash. Thus the P. yezoensis, appeared as a sensitive indicator, could be used as one of test organisms for determination of the biological effect of pollutants contaminated in marine environment.

Keywords

References

  1. Fletcher, R.L. 1991. Marine macroalgae as bioassay test organisms. In: Ectoxicology and the Marine Environment. Abel P.D. and V. Axiak eds., Ellis Horwood Ltd., New York, pp. 111-131
  2. Gonzales, R.A. and J.M. Widholm. 1985. Selection of plant cells for desirable characteristics: Inhibitor resistance. In: Plant Cell Culture: A Practical Approach. Dixon, R.A. ed., IRL Press, Washington D.C., pp. 67-78
  3. Hardisson, A, I. Frias and A. de Bonis. 1998. Mercury in algae of the Canary Islands littoral. Environ. International, 24, 945-950 https://doi.org/10.1016/S0160-4120(98)00081-6
  4. Jin, H.J., G.M. Seo, Y.C. Cho, E.K. Hwang, C.H. Sohn and Y.K. Hong. 1997. Gelling agents for tissue culture of the seaweed Hizikia fusiformis. J. Appl. Phycol., 9, 489-493
  5. Jin, L.G., J.S. Choi, H.J. Jin, H.W. Shin and Y.K. Hong. 2002. Isolation of pollutant (pine needle ash )-responding genes from the seaweed Porphyra yezoensis tissue. Fish. Sci., 68, S1044-S1047 https://doi.org/10.2331/fishsci.68.sup2_1044
  6. Kim, J.K. and T.J. Han. 2000. Effects of inorganic nutrients and heavy metals on reproduction of the green algae Ulva pertusa Kjellman. Algae, 15, 81-88
  7. Leborans, G.F. and A. Novillo. 1996. Toxicity and bioaccumulation of cadmium in Olisthodiscus luteus. Water Res., 30, 56-62
  8. Lobban, C.S., D.J. Chapman and B.P. Kremer. 1988. Experimental Phycology: A Laboratory Manual. Cambridge University Press, New York, 295pp
  9. Naldi, M. and P.A. Wheeler. 1999. Changes in nitrogen pools in Ulva fenestrata (Chlorophyta) and Gracilaria pacifica (Rhodophyta) under nitrate and ammonium enrichment. J. Phycol., 35, 70-77 https://doi.org/10.1046/j.1529-8817.1999.3510070.x
  10. Nam, B.H., H.J. Jin, S.K. Kim and Y.K. Hong. 1998. Quantitative viability of seaweed tissues assessed with 2, 3, 5-triphenyltetrazolium chloride. J. Appl. Phycol., 10, 31-36 https://doi.org/10.1023/A:1008059314639
  11. Park, J.W., Y.C. Cho, B.H. Nam, H.J. Jin, C.H. Sohn and Y.K. Hong. 1998. RAPD identification of genetic variation in the seaweed Hizikia fusiformis (Fucales, Phaeophyta). J. Mar. Biotechnol., 6, 62-64
  12. ProvasoIi, L. 1966. Media and prospects for the cultivation of marine algae. In: Cultures and Collections of Algae. Watanabe A. and A. Hattori eds., Jap. Soc. Plant Physiol., Tokyo, pp. 63-75
  13. Rioboo, C., O. Gonzalez, C. Herrero and A. Cid. 2002. Physiological response of freshwater microalga (Chlorella vulgaris) to triazine and phenylurea herbicides. Aquatic Toxicol., 59, 225-235 https://doi.org/10.1016/S0166-445X(01)00255-7
  14. Steidinger, K.A. 1983. A re-evaluation of toxic dinoflagellate biology and ecology. Prog. Phycol. Res., 2, 147-188
  15. Su, X and A. Gibor. 1988. A method for RNA isolation from marine macro-algae. Anal. Biochern., 174, 650- 657 https://doi.org/10.1016/0003-2697(88)90068-1
  16. NFRDI (National Fisheries Research and Development Institute). 2000. A report on the effect of mountain fire occuvred in the East coast on the costal fishing grounds, East Sea Fisherich Institute, Kwibin Publishing Co., Gangneung, Korea, 101 pp