Effects of Nicotine on mineralization in human fetal osteoblasts

니코틴이 사람태아골모세포의 광물화 과정에 미치는 영향

  • Lim, Sung-Woo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Han, Sang-Heon (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Lee, Seong-Jin (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Suk-Joo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 임성우 (원광대학교 치과대학 치주과학교실) ;
  • 한상헌 (원광대학교 치과대학 치주과학교실) ;
  • 이성진 (원광대학교 치과대학 치주과학교실) ;
  • 유석주 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실)
  • Published : 2003.12.31

Abstract

Nicotine is one of the major components of cigarette smoking which causes various systemic and local diseases to human body. The purpose of the present study was to investigate the effects of nicotine on bone mineralization in human fetal osteoblasts cell line(hFOB1). To compare the alkaline ph-osphatase(ALP) synthesis, hFOBl were cultured with DMEM/F-12 1:1 Mixture and 100 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, 1 ${\mu}g$/ml, 10 ${\mu}g$/ml, 100 ${\mu}g$/ml of nicotine. And to compare the calcium accumulation, hFOB1 cultured for 23 days were quantified and photographed. ALP activity of hFOB1 exposed to nicotine was not significantly changed at a lower concentrations of nicotine, but was significantly decreased at a higher concentrations (10 ${\mu}g$/ml, 100 ${\mu}g$/ml) of nicotine (p<0.05). A quantified calcium acculation in hFOB1 was significantly decreased at 1,10, and 100${\mu}g$/ml of nicotine (p<0.05). Significantly decreased calcium deposition was observed at 1, 10, and 100${\mu}$/ml of nicotine. These results indicate that a higher concentration of nicotine show a negative effects on mineralization of hFOB1.

Keywords

References

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