Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effects of Nicotine on mineralization in human fetal osteoblasts

니코틴이 사람태아골모세포의 광물화 과정에 미치는 영향

  • Lim, Sung-Woo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Han, Sang-Heon (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Lee, Seong-Jin (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Suk-Joo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 임성우 (원광대학교 치과대학 치주과학교실) ;
  • 한상헌 (원광대학교 치과대학 치주과학교실) ;
  • 이성진 (원광대학교 치과대학 치주과학교실) ;
  • 유석주 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실)
  • Published : 2003.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Nicotine is one of the major components of cigarette smoking which causes various systemic and local diseases to human body. The purpose of the present study was to investigate the effects of nicotine on bone mineralization in human fetal osteoblasts cell line(hFOB1). To compare the alkaline ph-osphatase(ALP) synthesis, hFOBl were cultured with DMEM/F-12 1:1 Mixture and 100 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, 1 ${\mu}g$/ml, 10 ${\mu}g$/ml, 100 ${\mu}g$/ml of nicotine. And to compare the calcium accumulation, hFOB1 cultured for 23 days were quantified and photographed. ALP activity of hFOB1 exposed to nicotine was not significantly changed at a lower concentrations of nicotine, but was significantly decreased at a higher concentrations (10 ${\mu}g$/ml, 100 ${\mu}g$/ml) of nicotine (p<0.05). A quantified calcium acculation in hFOB1 was significantly decreased at 1,10, and 100${\mu}g$/ml of nicotine (p<0.05). Significantly decreased calcium deposition was observed at 1, 10, and 100${\mu}$/ml of nicotine. These results indicate that a higher concentration of nicotine show a negative effects on mineralization of hFOB1.

Keywords

References

  1. 김재호, 신형식, 김탁 등 : 니코틴이 치은섬유아 세포의 세포주기 조절 단백질 발현 에 미치는 영향. 대한치주과학회지 31(3):597-611, 2001
  2. Haber J, Kent RL. : Cigarette smoking in a periodontal practice. J Periodontol 63(2):100-6, 1992
  3. Grossi SG, Zambon JJ, Ho AW, et al : Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. J Periodontol 65(3):260-7, 1994
  4. Preber H, Bergstrom J. : Effect of cigarette smoking on periodontal healing foll- owing surgical therapy. J Clin Periodontol 17(S):324-8, 1990
  5. Newman MG, Kornman KS, Holtzman S. : Association of clinical risk factors with treatment outcomes. J Periodontol 65(5 Suppl):489-97, 1994
  6. Feldman RS, Bravacos JS, Rose CL. : Association between smoking different tobacco products and periodontal disease indexes. J Periodontol 54(8):481-7, 1983
  7. Bergstrom J, Eliasson S, Preber H. : Cigarette smoking and periodontal bone loss. J Periodontol 62(4):242-6, 1991
  8. Preber H, Bergstorm J. : Effect of cigarette smoking on periodontal healing foll- owing surgical therapy. J Clin Periodontol 17:324-328, 1990
  9. Preber H, Bergstorm J. : Cigarette smoking in patients referred for periodontal treatment. Scan J Dent Res 94:102-108, 1986
  10. Goldminz D, Bennet RG. : Cigarette smoking to localized osteitis. J Oral Surg 37:732-735, 1979
  11. Hayesm Jr, W.J. : Pesticides Studied in Man, pp. 86-91. Williams & Wilkins, Baltimore (1982)
  12. Slotkin TA, Orband-Miller L, Queen KL, et al : Effects of prenatal Nicotine exposure on biochemical development of rat brain regions:Maternal drug infusi- on via osmotic minipump. J Pharmacol Exp Ther 240:602-611, 1987
  13. Konno S, Chiao JW, Wu JM. : Effects of Nicotine on cellular proliferation, cell cycle phase distribution, and macromolecular synthesis in human promyelocytic HL-60 leukemia cells, Cancer Lett(Netherlands) 33:91-97, 1986
  14. Tipton DA, Daddous MK, : Effects of Nicotine on proliferation and extracellul- ar matrix production of human gingival fibroblasts in vitro. J Periodontol 66:1056-1063, 1995
  15. Chamson A, Frey J, Hivert M. : Effects of tobacco smoke extracts on collagen biosynthesis by fibroblast cell cultures, J Toxicol Environ Health 9:921-932, 1982
  16. Hollinger JO, Schmitt JM, Hwang K, Soleymani P, Buck D. : Impact of nicoti- ne on bone healing. J Biomed Mater Res 15:45(4)294-301, 1999
  17. Pinto JR, Bosco AF, Okamoto T, Guerra JB, Piza IG. : Effects of nicotine on the healing of extraction sockets in rats. A histologic study. Braz Dent J 13(1): 3-9, 2002
  18. Nociti FH, Stefani CM, Sallum EA, et al : Nicotine and bone density around titanium implants : a histometric stduy in rabbits. Implant Dent 11(2): 176-82, 2002
  19. Fang MA, Frost PJ, Iida-Klein A, et al. : Effects of nicotine on cellular function in UMR 106-01 osteoblast-like cells. Clinical pharmacology of nicotine. Annu Rev Med 37:21-32, 1986
  20. Ramp WK, Lenz LG, Galvin RJ. : Nicotine inhibits collagen synthesis and alkaline phosphatase activity, but stimulates DNA synthesis in osteoblast-like cells. Proc Soc Exp Biol Med 197(1):36-43 ,1991 https://doi.org/10.3181/00379727-197-43221
  21. Benowitz NL. : Clinical pharmacology of nicotine. Annu Rev Med 37:21-32, 1986
  22. Clarke NG, Shepherd BC : The effects of epinephrine and Nicotine on gingival blood flow in the rabbit. Arch Oral Biol 29:789-793, 1984
  23. Mosely LH, Finseth F. : Cigarette smoking: impairment of digital blood flow and wound healing in the hand, Hand 9:97-100, 1977
  24. Kyerematen GA, Mongan ML, Chattopadhyay B, et al. : Disposition of nicotine and eight metabolites in smokers and nonsmokers : identification in smokers of two metabolites that are longer lived than cotinine. Clin. Pharmacol. Ther 48: 641-651, 1990
  25. Tipton DA, Dabbous MK. : Effects of nicotine on proliferation and extracellular matrix production of human gingival fibroblasts in vitro. J Periodontol 1991; 62 (2): 147-52 https://doi.org/10.1902/jop.1991.62.2.147
  26. Bissada NF, et al. : Neutrophil functional activity in juvenile and adult onset diabetic patients with mild and severe periodontitis. J Periodont Res 17:500-502, 1982
  27. Peacock ME, et al. : The effect of Nicotine on reproduction and attachment of human gingival fibroblast in vitro. J Periodontol 64:658-665, 1993
  28. Riebel GD, Boden SD, Whitesides TE, Hutton WC. : The effect of nicotine on incorporation of cancellous bone graft in an animal model. Spine. Oct 15;20(20): 2198-2202, 1995
  29. Daftari TK, Whitesides TE Jr, Heller JG, et al : Nicotine on the revasculariza- tion of bone graft. An experimental study in rabbits. Spine. Apr 15:19(8):904- 11, 1994
  30. Leblebicioglu-Bekcioglu B, Paulson RB, Paulson JO, et al : Effects of caffeine and nicotine administration on growth and ossification of the ICR mouse fetus. J Craniofac Genet Dev Biol Jul-Sep:15(3): 146-56, 1995
  31. Nash JE, Persaud TV. : Influence of nicotine and caffeine on skeletal develop- ment in the rat. Anat Anz 168(2): 109-26, 1989
  32. Nociti FH, Nogueira-Filho GR, et al : The influence of nicotine on the bone loss rate in ligature-induced periodontitis. A histometric stduy in rats. J Peri- odontol Sep:71(9):1460-4, 2000
  33. Luben RA, Wong GL, Cohn DV. : Biochemical characterization with parathro- mone and calcitonin of isolated bone cells : Provisional identification of osteobl- asts and osteoclasts. Endocrinology 99:526-534, 1976
  34. Bancroft JD, Stevens A. : Theory and practice of histological techniques, 3rd edition. Churchill Livingstone, New York 245-267, 1990
  35. Woods B, Kellaway TD. : Cutaneous calculi subepidermal calcified nodules. Br J Dermatol 75:1-11, 1963
  36. Dermis D.J. : Clinical dermatology, 22nd revision. Lippincott-Raven publishers, Philadelphia New York, unit 12-12:1-17, 1995