Applied Microscopy

  • 제33권3호
  • /
  • Pages.243-250
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  • 2003
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

넙치 (Paralichthys olivaceus)의 웅성생식세포 발달에 관한 미세구조적 연구

Ultrastructural Study on the Development of Male Germ Cell of the Olive Flounder, Paralichthys olivaceus (Teleostei: Pleuronectidae)

  • Kim, Jae-Won (Department of Marine Biology, Pukyong National University) ;
  • Kim, Bong-Seok (Biotechnology Research Center, National Fisheries Research and Development Institute) ;
  • Choi, Cheol-Young (Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health (NIH)) ;
  • Lee, Jung-Sick (Department of Fish Pathology, Yosu National University)
  • 발행 : 2003.09.01
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초록

넙치의 웅성생식세포의 발달과 정자 구조를 광학현미경과 투과전자현미경을 이용하여 관찰하였다. 곡정세관의 각 소낭내의 생식상피에서는 분열 증식중인 정원세포군이 염기성 염료에 미약하게 반응하기 시작한다. 정소조직의 계속적인 발달로 각 소낭내에는 분열증식중인 정모세포군을 관찰할 수 있다. 이후 소엽내강에는 상당수의 초기 정세포를 관찰할 수 있으며, 더욱 더 발달된 변태된 정자를 볼 수 있다. 투과전자현미경에 의한 관찰에서 간기의 정원세포는 세포질이 미약한 반면, 커다란 핵과 뚜렷한 인을 가진다. 제1정모세포의 핵내에서는 연접사 복합체 (synaptonemal complex)가 뚜렷하고 세포질내에서는 세포소기관이 증가한다. 제2정모세포의 핵질은 응축되어 높은 전자밀도를 나타낸다. 정세포는 세포질과 핵질이 응축되면서 타원형의 형태를 취하고, 미토콘드리아는 핵의 후방으로 위치한다. 변태를 마친 정자는 두부와 미부로 구성되며 무첨체형이다. 두부 후방에서는 cytoplasmic collar는 6개의 미토콘드리아를 가진다. 미부에서는 axonemal lateral fin을 관찰할 수 있다. 미부 편모 축사의 횡단면은 "9+2"의 미세소관 구조를 나타낸다.

Ultrastructural changes of the male germ cells and structure of spermatozoa in Paralichthys olivaceus were examined by means of the light and transmission electron microscopes. The spermatogonium has a large nucleus with a single nucleus with a single nucleolus in the interphase. Primary spermatocytes are identified by the formation of the synaptonemal complex in the karyoplasm. The secondary spermatocytes are more concentrated and contains numerous cell organelle in the cytoplasm. The nucleus of spermatid in spermiogenesis is more condensed in the karyoplasm, and show spherical structure in shape. Mitochondria of the spermatids are observed in the lower portion of the nucleus. The spermatozoon consists of the head, mid piece and tail. The acrosome is not observed in the head. Axial filaments of the flagellum consists of nine pairs of the peripheral microtubules and one pair of the central microtubules.

키워드

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