Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Changes of Growth, Morphology and Microcystin Production in Microcystis aeruginosa in Response to Zooplankton Culture Media Filtrate

동물플랑크톤 배양여과액에 의한 Microcystis aeruginosa의 성장,형태 및 microcystin 생성량의 변화

  • Ha, Kyong (Dept. of Biological Science, Silla University) ;
  • Jang, Min-Ho (School of Biological and Biomedical Science, University of Durham) ;
  • Jung, Jong-Mun (Dept. of Biology, Pusan National University) ;
  • Joo, Gea-Jae (Dept. of Biology, Pusan National University)
  • Published : 2003.03.31
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Abstract

Growth, colony formation and microcystin production of 'low-toxic' Microcysits aeruginosa $K{\"{u}}tzing$ were examined in relation to the 'info-chemicals' released by zooplankton. Algae were cultured in a medium with or without filtered water taken from cultures of Daphnia magna Straus (300 ind./L) or Moina macrocopa Straus (500 ind./L), The growth of M. aeruginosa, based on cell number, was also significantly different from populations cultured in the media with and without filtered zooplankton water from the exponential growth phase. In the 6-day experiment, the growth pattern of M. aeruginosa cultured with ZCMF was clearly different to control with-out ZCMF. Mean number of cells/particle and particle bio-volume of M. aeruginosa increased significantly from the day 2 for the Daphnia-CMF or Moina-CMF treat-ments. Microcystin production was promoted showing from 18.7 to 55 ${\mu}g/g$-dry cell in the zooplankton treatments relative to the controls. At peaked level on day 4, the highest level of up to $70.5{\pm}16.8\;{\mu}g/g$-dry cell was observed in the D. magna treatment. This study suggested that 'info-chemicals' from zooplankton might induce the increase of algal growth rates, colony formation and microcystin production, these seem to be advantageous to the alga and thus as a grazing defense mechanism.

동물플랑크톤 배양여과액 내의 분비물질 (info-chemi-cals)에 의해 유도되는 Microcystis aeruginosa의 성장, 군체형성과 독소생성량의 변화를 관찰하였다. 성체까지 자란 동물플랑크톤 Daphnia magna Straus와 Moina macrocopa Straus의 배양 여과액 (ZCMF)을 비교적 낮은 독소를 생성하는 M. aeruginosa배지에 첨가해준 후, ZCMF의 농도에 따른 성장시기별 세포밀도의 변화를 관찰한 결과, 지수성장기 (7일 이후)부터, ZCMF농도가 높아짐에 따라 세포밀도의 감소가 관찰되었다. 또한,20% ZCMF를 M. aeruginosa배지에 첨가해준 후, 군체형성 변화 및 microcystin 생성량의 변화를 관찰한 결과, 동물플랑크톤 처리군에서 2${\sim}$4일 째까지 군체당 세포수 및 평균 입자체적의 증가 현상이 관찰되었으며, 이 현상은 Moina 처리군보다는 Daphnia-처리군에서 더 뚜렷하게 관찰되었다. Microcystin 생성량의 경우 동물플랑크톤 처리군에서 4일째 최고값을 보여주었으며, 이후 감소하는 경향을 보였다. 특히, Daphnia 처리군에서는 4일째최고 $70.5{\pm}16.8\;{\mu}g/g$-dry cell까지 검출되었다. M. aeruginosa의 성장형의 변화, 군체형성과 microcystin 생성현상은 동물플랑크톤의 분비 화학물질을 매개로 한 M. aeruginosa의 방어수단으로 생각되며, 이러한 기작은 수생 생태계 내에서 피${\cdot}$포식자간의 상호관계를 설명할 수 있는 한 현상으로 생각된다.

Keywords

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