Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidant Activities of Glycyrrhizin and its Effect on Renal Expression of Na,K-ATPase in Gentamicin-induced Acute Renal Failure Rats

Glycyrrhizin의 항산화 활성 및 Gentamincin 유도 급성 신부전 백서 신장의 Na,K-ATPase 발현에 미치는 영향

  • Sohn Eun Jin (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kang Dae Gill (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee An Sook (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Yun Mi (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yin Ming Hao (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yeum Kee Bok (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Noh Suk Yun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Ho Sub (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 손은진 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 강대길 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이안숙 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이윤미 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 윤명호 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 염기복 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 노숙연 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이호섭 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2003.04.01
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Abstract

The present study was aimed to investigate whether glycyrrhizin, which is the major component of Glycyrrhiza uralensis, has an antioxidant effect and regulatory effect on Na,K-ATPase in gentamicin-induced acute renal failure (ARF) rats . It is well known that reactive oxygen species (ROS), such as superoxide anion and hydroxyl radical, are main pathophysiological factor in gentamicin-induced ARF. Glycyrrhizin showed potent in vitro antioxidant activity, especially superoxide scavenging activity, in a dose-dependent manner. Plasma lipid peroxide level was restored to normal level by oral administration of glycyrrhizin (200 mg/kg) in the gentamicin-induced ARF rats. The expression of Na,K-ATPase α1 subunit was restored in the gentamicin-induced ARF rats by administration of glycyrrhizin, whereas β1 subunit was not restored. The renal functional parameters including urine volume, cleatinine clearance, urine osmolality, solute-free water reabroption were also partially restored in gentamicin-ARF rats by administration of glycyrrhizin. Taken together, the amelioration of renal functions and the expression of sodium pump by administration of glycyrrhizin in the gentamicin-induced ARF was appear to be mediated by the scavenging of ROS.

Keywords

References

  1. Gen Phamacol v.26 Gentamicin nephrotoxicity in humans and animals: some recent research. Ali B.H.
  2. Arch Biophys v.205 Mechanisms of gentamicin induced dysfunction of renal cortical mitochondria. I. Effects on mitochondrial respiration. Weinberg, M.J.;Humes, D.H. https://doi.org/10.1016/0003-9861(80)90102-2
  3. Drug Metab Rev v.31 no.4 Oxidant mechanisms in toxic acute renal failure. Baliga, R.;Veda, N.;Walker, PD.;Shah, S.V. https://doi.org/10.1081/DMR-100101947
  4. Am J Physiol v.256 In vitro gentamicin of hydrogen peroxide by rat kidney cortex and glomeruli. Giudet, B.R.;Shah, S.V.
  5. Jpn J Phamacol v.85 Gentamicin decrease the abundance of Aquaporin water channel in rat kidney. Lee, J.U.;Yoo, K.S.;Kang, D.G.;Kim, S.W.;Choi, K.C.
  6. J Am Soc Nephrol v.12 Cisplatin decrease the abundance of AQP water channel in rat kidney. Kim, S.W.;Lee, J.U.;Nah, M.Y.;Kang, D.G.;Ahn, K.Y.;Lee, H.S.;Choi, K.C.
  7. Kidney lnt v.28 Monoclonal antibody to Na,K-ATPase: Immunocyto- chemical localization along nephron segments. 7. Kashdarian, M.;Biemesderfer, D.;Caplan, M., Forbush, B. https://doi.org/10.1038/ki.1985.216
  8. Am J Phsiol v.278 Dcreased abundance of major Na+ transporters in kidneys of rats with ischemia-induced acute renal failure. Kwon, T.H.;Frokiaer, J.;Han, J.S.;Knepper, M.A.;Nielsen, S.
  9. 동의생리병리학회지 v.15 no.5 Glycyrhhizin이 gentamicin 유도급성 신부전 백서의 腎臟 기능에 미치는 영향 강대길;손은진;홍성각;정헌택;이호섭
  10. 중약대사전 안덕균;이경순;신민교;김창민
  11. Endocrinol v.135 Glycyrrhizin induced mineralcorticoid activity through alterations in cortisol metabolism in the human kidney. Kageyama, T.;Suzuki, H.;Saruta, T. https://doi.org/10.1677/joe.0.1350147
  12. Am J Surg v.178 no.6 Attenuation of renal ischemia-reperfusion injury with selectin inhibition in a rabbit model. Sumramanian. S.;Bowyer, M.W.;Egan, J.C.;Knolmayer, T.J. https://doi.org/10.1016/S0002-9610(99)00238-X
  13. Phytomedicine v.6 no.6 Protective effects of Glycyrrhizae radix extract and its compounds in a renal hypoxia (ischemia)-reoxygenation (reperfusion) model. Yokozawa, T.;Liu, Z.W.;Chen, C.P. https://doi.org/10.1016/S0944-7113(00)80072-0
  14. 생약학회지 v.32 no.3 생약추출물에 의한 superoxide와 hydroxyl radical 소거능 검색 방법 개선 강대길;이호섭
  15. Anal Biochem. v.95 no.2 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Ohkawa, H.;Ohishi, N.;Yagi, K. https://doi.org/10.1016/0003-2697(79)90738-3
  16. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram of protein utilizing the principle of protein-dye binding. Bradford, M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  17. N Eng J Med v.5 no.30 Acute renal failure. Thadhini, R.;Pascual, M.;Bonventre J.V.M.
  18. J Pediat v.105 Physiologt of acute reanal failure Kon, V.;Ichikawa, I. https://doi.org/10.1016/S0022-3476(84)80004-9
  19. Ann Intern Med v.100 no.3 Risk factors for nephrotoxicity in patients treated with aminoglycosides. Moore, R.D.;Smith, C.R.;Lipsky, J.J.;Mellits, E.D.;Lietman, P.S. https://doi.org/10.7326/0003-4819-100-3-352
  20. Kidney Int v.40 no.2 Protective action of glycine in cisplatin nephrotoxicity. Heyman, S.N.;Rosen, S.;Silva, P.;Spokes, K.;Egorin, M.J.;Epstein, F.H. https://doi.org/10.1038/ki.1991.210
  21. 腎臟학 연세대腎臟질환연구소(편저)
  22. Appl Toxicol v.17 no.6 Effect of lipoic acid administratiom on gentamicin-induced lipid peroxidation in rats. Sanhya, P.;Varalskshmi, P. https://doi.org/10.1002/(SICI)1099-1263(199711/12)17:6<405::AID-JAT459>3.0.CO;2-3
  23. Arch Biochem Biophys v.205 no.1 Mechanisms of gentamicin-induced dysfunction of renal cortical mitochondria. II. Effects on mitochondrial monovalent cation transport. Weinberg, J.M.;Harding, P.G.;Humes, H.D. https://doi.org/10.1016/0003-9861(80)90103-4
  24. J Pharmacol Exp Ther v.247 no.3 Gentamicin inhibits agonist stimulation of the phosphatidylinositol cascade in primary cultures of rabbit proximal tubular cells and in rat renal cortex. Ramsammy, L.S.;Josepovitz, C;Kaloyanides, G.J.
  25. J Am Soc Nephrol v.12 no.1 Gentamicin inhibits renal protein and phospholipid metabolism in rats: implications involving intracellular trafficking. Sundin, D.P.;Sandoval, R.;Molitoris, B.A.
  26. Am J Phsiol v.266 Mechanisms for protective effects of free radical scavengers on gentamicin-mediated nephropathy in rats. Nakajima T.;Hischida A.;Kato A .
  27. J Clin Invest v.81 Evidence suggesting a role for hydroxyl radical in gentamicin-induced acute renal failure in rats. Walker, P.O.;Shah, S.V. https://doi.org/10.1172/JCI113325
  28. Am J Physiol v.263 no.6 Central hypertensinogenic effects of glycyrrhizic acid and carbenoxolone. Gomez-Sanchez, E. P.;Gomez-Sanchez, C. E.
  29. J Clin Endocrinol Metab v.80 no.6 3¬Monoglucuronyl-glycyrrhetinic acid is a major metabolite that caused licorice-induced psedoaldosteronism. Kato, H.;Kanaoka, M.;Yano, S.;Kobayashi, M. https://doi.org/10.1210/jc.80.6.1929
  30. Free Radic Biol v.23 no.2 Antioxidant constituents from licorice roots: isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Vaya, J.;Belinky, P.A.;Aviram, M. https://doi.org/10.1016/S0891-5849(97)00089-0
  31. J Appl Toxicol v.17 no.6 Effect of lipoic acid administration on gentamicin-induced lipid peroxidation in rats. Sandhya, P.;Varalakshmi, P. https://doi.org/10.1002/(SICI)1099-1263(199711/12)17:6<405::AID-JAT459>3.0.CO;2-3
  32. Annu Rev Pharmacol Toxicol v.28 Drug nephrotoxicity. Walker, RJ.;Duggin, G.G. https://doi.org/10.1146/annurev.pa.28.040188.001555
  33. J Lab Clin Med v.101 no.6 The renal concentrating defect after gentamicin administration in the rat. Gordon, J. A.;Dillingham, M. A.;Guggenheim, S. J.;Grossfeld, P.O.;Anderson, R.J.
  34. Am J Physiol v.240 no.6 Mineralocorticoid effects on Na,K-ATPase in individual nephron segments. Garg, L.C.;Knepper, M.A.;Burg, M.B.
  35. 인체생리학 김광진;김창주;김형진;박사훈
  36. 전정판 腎臟學 서울대학교 의과대학
  37. Pflugers Arc v.329 no.3 The localization of the Na,K-ATPase in the cells of rat kidney cortex. A study on isolated plasma membranes. Kinne, R.;Schmitz, J.E.;Kinne-Saffran, E. https://doi.org/10.1007/BF00586614
  38. Pflugers Arch v.349 no.1 Induction of Na,K- ATPase in the proximal and distal convolution of the rat nephron after uninephrectomy. Schmidt, U.;Dubach, U.C.
  39. J Biol Chem v.263 no.21 Tissue-specific and developmental Lingrel, J.B.;Kuntzweiler, T.
  40. Mol Cell Biochem v.145 no.1 Role of DL alpha-lipoic acid in gentamicin induced nephrotoxicity. Sandhya, P.;Mohandass, S.;Varalakshmi, P. https://doi.org/10.1007/BF00925707
  41. J Biol Chem v.269 no.31 Na,K-ATPase. Lingrel, J.B.;Kuntzweiler, T.