Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Bulbus Allii Macrostemi Extract on PKC activity in Pulmonary Vascular Endothelial Cells Damaged by XO/HX

산소자유기에 의해 손상된 혈관내피세포의 PKC 활성도에 미치는 해백 추출물의 효과(I)

  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Lee Ho Seung (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kang Gil Seong (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Seob (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 이호승 (원광대학교 한의과대학 생리학교실) ;
  • 강길성 (원광대학교 한의과대학 생리학교실) ;
  • 김인섭 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2003.04.01
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Abstract

To investigate the protective effect of Bulbus Allii Macrostemi (BAM) on the damage by pulmonary vascular endothelial cells by xanthine oxidase (XO)/hypoxanthine (HX)-induced oxygen free radical, Neutral Red (NR) and protein kinase c (PKC) activity assay were used. The results were obtained as follows ; The viability of vascular endothelial cells treated with XO/HX was decreased. And activation of PKC represented a maximal increase in group treated with XO/HX for 15 mins in vasvular pulmonary endothelial cells. But pretreated groups with BAM extracts were not inhibited the increase of PKC activation by XO/HX in a dose-dependent fashion. These results show that XO/HX elicits toxic effects in cultured pulmonary vascular endothelial cells, and suggest that BAM extract is very effective in the prevention of XO/HX-induced PKC activation.

Keywords

References

  1. 臨床本草學 辛民敎
  2. 本草學 金先熙(外)
  3. 本草學 東醫學硏究所
  4. 韓國 本初圖鑑 安德均
  5. 中葯辭海 責編組(外)
  6. 中國道地葯材原色圖說 胡世林
  7. 本草從新 金昌增
  8. 實用植物本草 柳庚華(外)
  9. 中葯學 高學敏
  10. 中草藥學 上海中醫學院
  11. 東醫方劑學 尹古榮
  12. 全國重名易混中葯鑒手冊 崔同寅
  13. 中國葯材學(上冊) 正林生(外)
  14. 要草備要解釋 圖光韓醫次 圖書部
  15. J. Biol. Chem. v.259 Iron-catalyzed hydroxyl radical formation: Stringent requirement for free iron coordination site. Craf E.;Mahoney J. R.;Bryant R. C.;Eaton J. W.
  16. J Neurochem. v.51 Excitatory amino acid release from rat hippocampal slices as a consequence of free-radical formation. Pellegrini-Ciampietro D. E.;Cherici C.;Alesianl M.;Carla V.;Morroni F. https://doi.org/10.1111/j.1471-4159.1988.tb01187.x
  17. J. Neurosic. v.10 Excitatory amino acid and free radical formation may cooperate in the genesis of ischemia-induced neuronal damage. Pellegrini-Ciampietro D. E.;Cherici C.;Alesianl M.;Carla V.;Morroni F. https://doi.org/10.1523/JNEUROSCI.10-03-01035.1990
  18. 대한동의생리병리학회지 v.15 no.4 朱笑散 전탕액이 XO/HX에 의해 손상된 배양 심근세포 박동수에 미치는 영향 한동훈;권강범;황인진;김우경;김희찬;오광수;이호승;류도곤
  19. 대한동의생리병리학회지 v.15 no.4 丹參飮 전탕액이 산소자유기에 의해 손상된 심근세포의 LDH 활성도에 미치는 영향 손창식;권강범;정종선;황인진;김우경;김희찬;오광수;이호승;류도곤
  20. 대한동의생리병리학회지 v.15 no.5 甘豆湯 및 加味 甘豆湯 전탕액이 배양 심근세포의 LDH 활성도에 미치는 영향 박준배;권강범;김우경;오광수;김인규;이호섭;김영운;금경수;류도곤
  21. 朱笑散 전탕액과 구성약물이 배양 심근세포의 LDH 활성도에 미치는영향 한동훈;권강범;김우경;오광수;김인규;류도곤
  22. 동의생리병리학회지 v.16 no.2 과루薤白반하함 추출물이 배양 심근세포의 박동수와 LDH 활성도에 미치는 영향 안효창;권강범;박은영;장승효;류도곤
  23. Acta Histochem v.9 Cytology and cytochemistry of mammalian mytocardial cells in culture. Kasten FH.
  24. J. Biol. Chem. v.265 Modulation of the insulin factor II/mannosr6-phosphate receptor in microvascular endothelial cells by phobol ester via protein kinase C. Hu K.Q.;Backer J. M.;sahagian G.;Feener E. P.;King G.L.
  25. 漢藥의 藥理.成分.臨床應用. 金成萬(外)
  26. 中葯現大硏究與應用 王筠默(外)
  27. J. Biol. Chem. v.245 Quantitative aspects of the Production of superoxide anion radical by milk xanthine oxidase. Fridovich J.
  28. J. Biol. Chem. v.252 Liposome oxidation and erythrocyte lysis by enzymatically generated superoxide and hydrogen peroxide. Killogg E. W.;Fridovich J.
  29. Proc. Roy. Soc. London. v.147 The catalytlc decomposition of hydrogen peroxide by anion salts. Harber F.;Weiss J.
  30. J. Biol. Chem. v.259 Iron-catalyzed hydroxyl radical formation: Stringent requirement for free iron coordination site. Craf E.;Mahoney J. R.;Bryant R. C.;Eaton J. W.
  31. 소아과 v.42 no.10 허혈 유도에 의해 손상된 신생쥐의 배양 대뇌신경세포에 대한 vitamin E와 Desferrioxamine 보호효과. 이소라;오연균;박승택
  32. J. Clin. Invest. v.85 Increased endothelial albumin permeability mediated by protein kinase C activation. Lynch J.J.;Ferro T. J.;Blumenstork F. A.;Brockenauer A.M.;Malik A. M. https://doi.org/10.1172/JCI114663
  33. Stroke Suppl III v.21 70-kDa heat shock protein and c-fos gene expression after transient ischemia Nowak T. S.;Ikeda J.;Nakajima T.