동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제17권3호
  • /
  • Pages.804-809
  • /
  • 2003
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

가감보중익기탕의 LPS 유도 염증성 매개물에 대한 억제 효과

Kagam-bojungikgitang Inhibits LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages

  • 장선일 (임뮤노피아 부설연구소) ;
  • 김형진 (임뮤노피아 부설연구소) ;
  • 김용준 (임뮤노피아 부설연구소) ;
  • 배현옥 (원광대학교 의약자원연구센터) ;
  • 정헌택 (임뮤노피아 부설연구소) ;
  • 정옥삼 (원광대학교 약학대학 생약학교실) ;
  • 김윤철 (임뮤노피아 부설연구소) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실)
  • Jang Seon Il (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Kim Hyung Jin (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Kim Young Jun (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Pae Hyun Ock (Medical Resources Research Center, Wonkwang University) ;
  • Chung Hun Taeg (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Jeong Ok Sam (College of Pharmacy, Wonkwang University) ;
  • Kim Youn Chul (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Yun Yong Gab (Department of Oriental Medical Prescription, Wonkwang University)
  • 발행 : 2003.06.01
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초록

Kagam-bojungikgitang is the water extracts prepared from Ginseng Radix, Astragali Radix. Angelicae gigantis Radix, Astractylodis Rhizoma alba, Aurantii nobilis Pericarpium, Glycyrrhizae Radix, Artemisiae iwayomogii Herba, and Scutellariae Radix. This is a modified prescription of Bojungikgitang, which has been used for the treatment of indigestion, and immunological disease in oriental countries. In this study, the effects of Kagam-bojungikgitang and Bojungikgitang on the production of prostaglandin E₂ (PGE₂) and the expression of cyclooxygenase-2 (COX-2) were examined using RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Both prescriptions dose-dependently reduced the release of PGE2 and expression of COX-2 caused by stimulation of LPS without cytotoxic effect. Kagam-bojungikgitang's inhibitory effects were better than Bojungikgitang in PGE2 production and COX-2 expression. Moreover, Kagam-bojungikgitang also attenuated markedly the production of tumor necrosis factor (TNF)-α, and IL-6 than Bojungikgitang in LPS-stimulated RAW 264.7 macrophages. These results suggest that Kagam-bojungikgitang decreases PGE2 and pro-inflammatory cytokine production in macrophages and these properties may contribute to the anti-inflammatory activity of Kagam-bojungikgitang.

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