Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Kagam-bojungikgitang Inhibits LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages

가감보중익기탕의 LPS 유도 염증성 매개물에 대한 억제 효과

  • Jang Seon Il (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Kim Hyung Jin (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Kim Young Jun (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Pae Hyun Ock (Medical Resources Research Center, Wonkwang University) ;
  • Chung Hun Taeg (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Jeong Ok Sam (College of Pharmacy, Wonkwang University) ;
  • Kim Youn Chul (Immunopia Research Laboratory of Molecular and Cellular Immunology) ;
  • Yun Yong Gab (Department of Oriental Medical Prescription, Wonkwang University)
  • 장선일 (임뮤노피아 부설연구소) ;
  • 김형진 (임뮤노피아 부설연구소) ;
  • 김용준 (임뮤노피아 부설연구소) ;
  • 배현옥 (원광대학교 의약자원연구센터) ;
  • 정헌택 (임뮤노피아 부설연구소) ;
  • 정옥삼 (원광대학교 약학대학 생약학교실) ;
  • 김윤철 (임뮤노피아 부설연구소) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Kagam-bojungikgitang is the water extracts prepared from Ginseng Radix, Astragali Radix. Angelicae gigantis Radix, Astractylodis Rhizoma alba, Aurantii nobilis Pericarpium, Glycyrrhizae Radix, Artemisiae iwayomogii Herba, and Scutellariae Radix. This is a modified prescription of Bojungikgitang, which has been used for the treatment of indigestion, and immunological disease in oriental countries. In this study, the effects of Kagam-bojungikgitang and Bojungikgitang on the production of prostaglandin E₂ (PGE₂) and the expression of cyclooxygenase-2 (COX-2) were examined using RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Both prescriptions dose-dependently reduced the release of PGE2 and expression of COX-2 caused by stimulation of LPS without cytotoxic effect. Kagam-bojungikgitang's inhibitory effects were better than Bojungikgitang in PGE2 production and COX-2 expression. Moreover, Kagam-bojungikgitang also attenuated markedly the production of tumor necrosis factor (TNF)-α, and IL-6 than Bojungikgitang in LPS-stimulated RAW 264.7 macrophages. These results suggest that Kagam-bojungikgitang decreases PGE2 and pro-inflammatory cytokine production in macrophages and these properties may contribute to the anti-inflammatory activity of Kagam-bojungikgitang.

Keywords

References

  1. 東垣醫集-內外傷辨惑論 李東垣
  2. 東垣醫集-脾胃論 李東垣
  3. 方劑學 韓醫科大學方劑學敎授
  4. 東醫方劑와 處方解說 尹用甲
  5. 中醫歷代名方集成 裘沛然
  6. 대전대학교대학원 석사학위논문 補中益氣湯 및 少陰人 補中益氣湯이 S-180에 대한 항종양효과와 Cyclophosp hamide에 의한 부작용에 미치는 영향 金秀鎭
  7. 東義大學校大學院 博士學位論文 補中益氣湯 및 補中益氣湯 加味方이 흰쥐의 알레르기 천식에 미치는 영향 李承龍
  8. 경희대학교대학원 석사학위논문 補中益氣湯의 항스트레스효과에 대한 실험적 연구 金泰燁
  9. 경희대학교대학원 석사학위논문 少陰人 補中益氣湯과 後世方 補中益氣湯이 陽虛病證에 미치는 影響 安光武
  10. 圓光大學校大學院 博士學位論文 補中益氣湯 및 加減方이 家兎의 적출자궁, 장 및 혈관운동에 미치는 영향 尹用甲
  11. J Ethnopharmacol. Inhibition of LPS plus IFN-𝞬 induced inflammatory Mediators in RAW 264.7 Macrophage by Scoparone. Jang S.I;Kim H.J;Kim Y.J;Chung H.T;Yun Y.G;Kang T.H;Kim Y.C
  12. Cell. Phytother Res. Scopoletin from Artemisia feddei Inhibits COX-2 Expression ed PGE2, TNF-𝞪, IL-1𝞫, IL-6 production in RAW 264.7 Kim H.J;Jang S.I;Kim Y.J;Chung H.T;Yun Y.G;Kang T.H;Kim Y.C
  13. Immunopharm Immunot. Hepatoprotective effect of Bacalin, a major flavone from Scutellaria radix, on acetaminophen induced liver injury in mice. Jang S.I;Kim H.J;Hwang K.M;Jekal S.J;Pae H.O;Choi B.M;Yun Y.G;Kwon T.O;Chung H.T.
  14. 대한 한의학 방제학 교지 Bojungikgitang Inhibits LPS plus IFN-r induced inflammatory Mediators in RAW 264.7 Macrophage. Jang S.I;Kim H.J;Kim Y.J;Pae H.O;Chung H.T;Yun Y.G;Jeong O.S;Kim Y.C.
  15. Biochem Biophys Res Commun. v.282 Nitric oxide as a bioregulator of apoptosis. Chung H.T.;Pae H.O.;Choi B.M.;Billiar T.R.;Kim Y.M.
  16. J Rheumatol. v.24 no.SUP.49 The discovery and function of COX-2. Needleman P.;Isakson P.C.
  17. Int Immunopharmacol. v.1 Immunoferon, a glycoconjugate of natural origin, inhibits LPS-induced TNF-alpha production and inflammatory responses. Brieva A.;Guerrero A.;Alonso-Lebrero J.L.;Pivel J.P.
  18. Ann N Y Acad Sci. v.962 Mechanisms of hepatoprotection by nitric oxide. Wang Y.;Vodovotz Y.;Kim P.K.;Zamora R.;Billiar T.R.
  19. Fitoterapia. v.73 Inhibition of Trypanosoma cruzi growth by medical plant extracts Schinella G. R.;Tournier H. A.;Prieto J. M.;Ri;os J. L.;Buschiazzo H.;Zaidenberg A.
  20. Neurobiol Learn Mem. v.79 Decursin from Angelica gigas mitigates amnesia induced by scopolamine in mice. Kang S.Y.;Lee K.Y.;Park M.J.;Kim Y.C.;Markelonis G.J.;Oh T.H.;Kim Y.C.
  21. Biorheology. v.40 Effect of angelica on the expressional changes of cytokines in endothelial cells induced by hyperlipidemic serum. Jing-Ping O.Y.;Baohua W.;Yongming L.;Lei W.;Jingwei Y.
  22. Am Surg. v.68 In vitro immunomodulatory effects of herbal products. Wilasrusmee C.;Siddiqui J.;Bruch D.;Wilasrusmee S.;Kittur S.;Kittur D.S.
  23. Planta Med. v.65 Scopoletin: an inducible nitric oxide synthesis inhibitory active constituent from Artemisia feddei. Kang T.H.;Pae H.O.;Jeong S.J.;Yoo J.C.;Choi B.M.;Jun C.D.;Chung H.T.;Miyamoto T.;Higuchi R.;Kim Y.C.
  24. Eur J Pharmacol. v.425 Inhibition of TPA-induced cyclooxygenase-2 expression and skin inflammation in mice by wogonin, a plant flavone from Scutellaria radix. Park B.K.;Heo M.Y.;Park H.;Kim H.P.
  25. Food Chem Toxicol. v.40 Direct scavenging of nitric oxide and superoxide by green tea. Nakagawa T.;Yokozawa T.
  26. J Ethnopharmacol. v.76 Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide productions by murine macrophage cell line, RAW 264.7 cells. Seo W.G;Pae H.O.;Oh G.S.;Chai K.Y.;Kwon T.O.;Yun Y.G.;Kim N.Y,;Chung H.T.
  27. Int Immunopharmacol. v.2 Inhibition of TNF-alpha, IL-1beta, and IL-6 productions and NF-kappaB activation in lipopolysaccharide-activated RAW 264.7 macrophages by catalposide, an iridoid glycoside isolated from Catalpa ovata G. Don (Bignoniaceae). An S.J.;Pae H.O.;Oh G.S.;Choi B.M.;Jeong S.;Jang S.I.;Oh H.;Kwon T.O.;Song C.E.;Chung H.T.