Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Yanggyuksanhwa-tang on Pyramidal Neuron and HSP72 Expression in Ischemic Damaged Hippocampus of Aged BCAD Rats

노령 흰쥐의 뇌허혈 손상시 양격산화탕이 뇌해마의 신경세포 및 HSP72 발현에 미치는 영향

  • Park Eun Kyung (Department of Anatomy, College of Oriental Medicine, Kyunghee University) ;
  • Shin Jung Won (Department of Neuroscience, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Sohn Young Joo (Department of Neuroscience, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Jung Hyuk Sang (Department of Anatomy, College of Oriental Medicine, Kyunghee University) ;
  • Won Ran (Department of Neuroscience, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Sohn Nak Won (Department of Neuroscience, Graduate School of East-West Medical Science, Kyunghee University)
  • 박은경 (경희대학교 한의과대학 해부학교실) ;
  • 신정원 (경희대학교 동서의학대학원 신경과학교실) ;
  • 손영주 (경희대학교 동서의학대학원 신경과학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실) ;
  • 원란 (경희대학교 동서의학대학원 신경과학교실) ;
  • 손낙원 (경희대학교 동서의학대학원 신경과학교실)
  • Published : 2003.06.01
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Abstract

This study investigated the effect of Yanggyuksanhwa-tang on cerebral ischemia of the rats. Considering age-related impact on cerebral ischemia, aged rats (18 months old) were used for this study. Ischemic damage was induced by the transient occlusion of bilateral common carotid arteries (BCAO) under the hypotension. Yanggyuksanhwa-tang was administered twice orally. Then changes of pyramidal neurons and heat shock protein 72 (HSP72) expressions in ischemic damaged hippocampus were of observed. The BCAO in aged rats led significant decrease of pyramidal neurons in CA1 hippocampus. While the treatment of Yanggyuksanhwa-tang significantly attenuated the reduction of pyramidal neurons in CA1 hippocampus following BCAO ischemia. The BCAO in aged rats led significant increase of HSP72 expression in CA1 and mild in CA3 hippocampus. While the treatment of Yanggyuksanhwa-tang significantly attenuated the increase of HSP72 expression in CA1 hippocampus following BCAO ischemia. The extent of HSP72 expression in CA2 and DG of hippocampus was not different between the sham operated group, the BCAO ischemia control group, and the group of Yanggyuksanhwa-tang administration after BCAO ischemia. The treatment of Yanggyuksanhwa-tang significantly attenuated the increase of normalized optical density depending on HSP72 expression in CA1 hippocampus following BCAO ischemia.

Keywords

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