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The Anticancer Effects and Immune Response on the Metastatic Lung Cancer by Wanpae-tang  

Lee Seon Goo (Department of Pathology Oriental Medicine Sangji University)
Lee Dong Joo (Department of Pathology Oriental Medicine Sangji University)
Yoon Keun Chan (Department of Pathology Oriental Medicine Sangji University)
Ha Jee Yong (Department of Pathology Oriental Medicine Sangji University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.3, 2003 , pp. 711-718 More about this Journal
Abstract
Wanpae-tang is suggested to have antitumor activity on lung cancer. This study was performed to investigate antitumor, immune response, and apoptotic effects by Wanpae-tang in the cancer cell lines and C57BL/6 mice. Experimental studies were progressed through the anticancer activities such as, survival time, cell cytotoxicity, natural killer cell activity, productivity of interleukins and apoptotio effects. The results were summarized as follows: 1. Median survival time of Wanpae-tang treated group was prolonged to 4.1%, as compared with control group, but was not significant. 2. On the MTT assay, half-maximal inhibitory concentration(IC50) of Wanpae-tang was 15.00 ㎎/㎖ in HeLa cell, and 4.158 ㎎/㎖ in HRT-18 cell. 3. Natural killer cell activity in Wanpae-tang treated group was decreased in case of 100:1 and 10:1 effect cell/target cell ratio. 4. Production of interleukin-2, 4, 12 in Wanpae-tang treated group were significantly increased. 5. On the studies of Wanpae-tang induced apoptosis, a DNA fragmentation patterns were not appeared.
Keywords
Wanpae-tang(完肺湯); IC50; anti-tumor; immunomodulatory; IL-2; IL-4; IL-12; natural killer cell activity; apoptosis; DNA fragmentation;
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