Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Linkage Map and Quantitative Trait Loci(QTL) on Pig Chromosome 6

돼지 염색체 6번의 연관지도 및 양적형질 유전자좌위 탐색

  • Lee, H.Y. (National Livestock Research Institute, RDA) ;
  • Choi, B.H. (National Livestock Research Institute, RDA) ;
  • Kim, T.H. (National Livestock Research Institute, RDA) ;
  • Park, E.W. (National Livestock Research Institute, RDA) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Lee, H.K. (Department of Biotechnology, Hankyong National University) ;
  • Jeon, G.J. (Department of Biotechnology, Hankyong National University) ;
  • Cheong, I.C. (National Livestock Research Institute, RDA) ;
  • Hong, K.C. (Division of Biotechnology and Genetic Engineering, College of Life and Environmental Sciences Korea University)
  • 이혜영 (농촌진흥청 축산기술연구소) ;
  • 최봉환 (농촌진흥청 축산기술연구소) ;
  • 김태헌 (농촌진흥청 축산기술연구소) ;
  • 박응우 (농촌진흥청 축산기술연구소) ;
  • 윤두학 (농촌진흥청 축산기술연구소) ;
  • 이학교 (한경대학교 생명공학과) ;
  • 전광주 (한경대학교 생명공학과) ;
  • 정일정 (농촌진흥청 축산기술연구소) ;
  • 홍기창 (고려대학교 생명유전공학부)
  • Published : 2003.12.31
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Abstract

The objective of this study was to identify the quantitative traits loci(QTL) for economically important traits such as growth, carcass and meat quality on pig chromosome 6. A three generation resource population was constructed from cross between Korean native boars and Landrace sows. A total of 240 F$_2$ animals were produced using intercross between 10 boars and 31 sows of F$_1$ animals. Phenotypic data including body weight at 3 weeks, backfat thickness, muscle pH, shear force and crude protein level were collected from F$_2$ animals. Animals including grandparents(F$_0$), parents(F$_1$) and offspring(F$_2$) were genotyped for 29 microsatellite markers and PCR-RFLP marker on chromosome 6. The linkage analysis was performed using CRI-MAP software version 2.4(Green et al., 1990) with FIXED option to obtain the map distances. The total length of SSC6 linkage map estimated in this study was 169.3cM. The average distance between adjacent markers was 6.05cM. For mapping of QTL, we used F$_2$ QTL Analysis Servlet of QTL express, a web-based QTL mapping tool(http://qtl.cap.ed.ac.uk). Five QTLs were detected at 5% chromosome-wide level for body weight of 3 weeks of age, shear force, meat pH at 24 hours after slaughtering, backfat thickness and crude protein level on SSC6.

본 연구는 돼지의 염색체 6번에 존재하는 주요 경제형질에 관여하는 양적형질 유전자좌위(Quantitative trait loci; QTL)를 밝히기 위하여 초위성체 마커를 이용하여 유전자지도를 작성하였다. 기준집단의 조성은 재래돼지 수컷 5마리와 Landrace 암컷 9마리를 자연교미하여 생산된 F$_1$의 동복 자손별로 수컷 1두를 임의적으로 선발하여 암컷 2두 이상과 전형매 교배시켜 F$_2$ 240두를 생산하였고 QTL 분석에는 F$_2$ 183두만을 이용하였다. 기준집단의 표현형 성적은 3주령체중, 등지방두께, 도축 24시간 후의 pH, 전단력, 조단백질 함량 등을 조사 분석하였다. F$_2$ 집단은 재래돼지와 Landrace의 두 종의 평균성적을 갖고 있었으며, 개체간 표현형적 변이가 매우 높아서 경제형질과 연관된 QTL을 탐색하기 적절한 기준집단이라고 평가되었다. 연관지도는 29개의 초위성체 마커와 1개의 PCR-RFLP 마커(AMPKα2)를 이용하여 작성하였으며, 연관지도상의 염색체 길이는 169.3cM이었고, 마커간 평균 간격은 6.05cM이었다. 염색체 6번에서 경제형질과 연관된 유의적인 QTL은 모두 5개가 탐색 되었다. 3주령 체중과 연관된 QTL이 5cM 위치에서 탐색되었으며, 전단력, 도축 24시간 후 pH, 등지방두께, 조단백질 함량 등의 육질관련 QTL이 서로 다른 위치에서 5% 수준의 통계적 유의성이 확인되었다.

Keywords

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