DOI QR코드

DOI QR Code

Cryopreservation of Sporothalli of the Genus Porphyra (Bangiales, Rhodophyta) from Korea


초록

Cryopreservation of sporothalli of the red alga Porphyra (P. seriata, P. yezoensis, P. tenera, P. pseudolinearis and P. dentata) was performed by the two-step cooling method in liquid nitrogen. The algal samples were suspended in various cryoprotective solutions, and slowly cooled to -40$^{\circ}C$ in 4 hours using a programmed freezer. After the first slow cooling the suspensions with cryoprotectants were immediately immersed in liquid nitrogen. The suspension from the programmed freezer was thawed quickly by agitation of the vial in a water bath at 40°C. When ice in the suspension of cryogenic vial was mostly melted, the vial was transferred to an ice bath for complete melting of the residual ice. The cryoprotectants in the vial were washed off by gradual dilution with seawater. The viability of the cell was assessed with neutral red staining. The viability of Porphyra samples ranged 54.6-70.9% when the mixed suspension of 10% dimethylsulfoxide and 0.5 M sorbitol in 50% seawater used as a cryoprotectant.

키워드

참고문헌

  1. Bajaj Y.P.S. and Reinert J. 1977. Cryobiology of plant cell cultures and establishment of gene-banks. In: Reinert J. and Bajaj y.P.5. (eds), Appliedandfundametal aspects of plant cell, tissue, and organ culture. Springer Verlag, Berlin. pp. 757-777
  2. Ben-Amotz A. and Gilboa A. 1980a. Cryopreservation of marine unicellular algae. I. A survey of algae with regard to size, culture age, photosynthetic activity and chlorophyll-to-cell ratio. Mar. Ecol. Prog. Ser. 2: 157-161 https://doi.org/10.3354/meps002157
  3. Ben-Amotz A. and Gilboa A. 1980b. Cryopreservation of marine unicellular algae. II. Induction of freezing tolerance. Mar. Ecol. Prog. Ser. 2: 221-224 https://doi.org/10.3354/meps002221
  4. Bousfield I.J. 1984. Maintenance of industrial and marine bacteria and bacteriophages, In: Kirsop B.E. and Snell J.J.S. (eds), Maintenance of microorganisms. Academic Press, New York. pp. 63-68
  5. Chen T.H.H., Kartha K.K, Constabel F. and Gusta L.V. 1984a. Freezing characteristics of cultured Catharanthus roseus (L). G. Don cells treated with dimethylsulfoxide and sorbitol in relation to cryopreservation. PlantPhysiol. 75: 720-725 https://doi.org/10.1104/pp.75.3.720
  6. Chen T.H.H., Kartha K.K, Leung N.R, Kurz W.G.W., Chatson KB. and Constabel F. 1984b. Cryopreservation of alkaloid producing cell cultures of periwinkle (Catharanthus roseus). Plant Physiol. 75: 726-731 https://doi.org/10.1104/pp.75.3.726
  7. Fahy G.M., MacFarlane D.R, Angell CA. and Merymann H.T. 1984. Vitrification as an approach to cryopreservation. Cryobiology 21: 407-426 https://doi.org/10.1016/0011-2240(84)90079-8
  8. Fenwick C. and Day J.G. 1992. Cryopreservation of Teiraselmis suecica cultured under different nutrients regimes. J. Appl. Phycol. 4: 105-109 https://doi.org/10.1007/BF02442458
  9. Fujiyoshi E., Yamasaki M. and Kito H. 1993a. Cryopreservation of conchocelis of Porphyra yezoensis form. narawaensis. Suisanzoshoku 41: 85-87 (in Japanese with English abstract)
  10. Fujiyoshi E., Yamasaki M., Umezawa S. and Kito H. 1993b. Prefreezing conditions for cropreservation of conchocelis of Porphyra yezoensis form. narawaensis. Suisanzoshoku 41: 547-551(in Japanese with English abstract)
  11. Fujiyoshi E. 1997. Cryopreservation on Porphyra. Nat. Hist. Res. Special Issue No.3: 83-87
  12. Kono S., Kuwano K, Ninomiya M., Onishi J. and Saga N. 1997. Cryopreservation of Enteromorpha intestinalis (Ulvales, Chlorophyta) in liquid nitrogen. Phycologia 36: 76-78 https://doi.org/10.2216/i0031-8884-36-1-76.1
  13. Kono S., Kuwano K. and Saga N. 1998. Cryopreservation of Eisenia bicyclis (Laminari ales, Phaeophyta) in liquid nitrogen.j. Mar. bioteph. 6: 220-223
  14. Kuwano K and Saga N.,2000, Cryopreservation of marine algae: Applications in biotechnology. In: Fingerman M. and Nagabhushanam R. (eds), Recent advances in marine biotechnology. Volume 4: AqiiacuUure. Part A, Seaweeds and invertevrates, Science Publishers Inc., New Hampshire. pp. 23-40
  15. Kuwano K, Aruga Yiand Saga N.1992. Preliminary study on cryopreservationof the .conchocelts of Porphyra yezoensis. Fish. Sci. 58: 1793-8 https://doi.org/10.2331/suisan.58.1793
  16. Kuwano K, Aruga Y. and Saga N. 1993. Cryopreservation of the conchocelis of the marine alga PorphY,k yekensis Ueda (Rhodophyta) in liquid nitrogen. Plant Sci: 94: 215-25 https://doi.org/10.1016/0168-9452(93)90022-R
  17. Kuwano K, Aruga Y. and Saga N. 1994. Cryopreservation of the conchocelis of Porphyra (Rhodophyta) by applying a simple prefreezing system.J. Phycol. 30: 566-570 https://doi.org/10.1111/j.0022-3646.1994.00566.x
  18. Kuwano K, Aruga Y. and Saga N. 1996. Cryopreservation of the clonal gametophytic thalli of Porphyra (Rhodophyta). Plant Sci.116: 117-124 https://doi.org/10.1016/0168-9452(96)04380-4
  19. Leeson E. A., Cann J. P. and Morris G. J. 1984. Maintenance bf algae and protozoa, In: Kirsop B. E. and Snell J,J.S. (eds), Maintenance of,Microorganisms. Academic Press, London. pp.131-160
  20. Mazur P. 1984. Freezing of living cells: Mechanisms and implications. Am.J. Physiol. 247:C125-C142
  21. Migita S. 1964. Freeze-preservation of Porphyra thalli in viable state-I. Bull. Fac. Fish. Nagasaki Univ. 17: 44-54 (in Japanese with English summary)
  22. Migita S. 1966. Freeze-preservation of Porphyra thalli in viable state-H. Effect of cooling velocity and water content of thalli on the frost resistance. Bull. Fac. Fish. Nagasaki Univ. 21: 131-138(in Japanese with English abstract)
  23. Migita S. 1967. Viability and spore-liberation of conchocelisphase, Porphyra ienera, freeze-preserved in seawater. Bull. Fac. Fish. Nagasaki Univ. 22: 33-43 (in Japanese with English abstract)
  24. Miura A. 1992. Nori (Porphyra yezoensis). In: Miura A. (ed.), Cultivation of edible algae in Japan. Koseisha Koseikaku, Tokyo. pp. 11-24 (in Japanese)
  25. Morris G.J. 1978. Cryopreservation of 250 strains of Chlorococcales by the method of two-step cooling. Br. Phycol. J. 13: 15-24 https://doi.org/10.1080/00071617800650031
  26. Mumford T.F. and Miura A. 1988. Porphyra as food: cultivation and economics. In: Lembi C.A. and Waaland J.R, (eds), Algae and human affairs. Cambridge Univ, Press, Cambridge. pp. 87-117
  27. Provasoli, L. 1966. Media and prospects for the cultivation of marine algae. In: Watanabe A. and Hattori A. (eds), Cultures and collections of algae, Proc. U.S. - Japan Conf., Hakone, September 1%6. Japan. Soc. Plant Physiol., Tokyo. pp,63-75
  28. Renard P., Arbault S., Kaas R and Perez R 1992.A method for the cryopreservation of the gametophytes of the food algae Undaria pinnatifida (Laminariales). C.R. Acad. Sci. Serie (Paris) III. Sci. de la Vie 315: 445-451
  29. Saga N., Sakanishi Y. and Ogishima T. 1989. Method for quick evaluation of cell viabillity in marine macroalgae. Jpn. J. Phycol. 129-136
  30. Sakai A. and Sugawara Y. 1978. Survival of plant germlasm in liquid nitrogen. In: Li P. H. and Sakai A. (eds), Plant cold hardness andfreezing stress. Academic Press, New York. pp. 345-359
  31. Sakanishi Y. and Saga N. 1994.Survival of female gametophytic cells of Laminaria diabolica Miyabe (Phaeophyta) in liquid nitrogen. Fish. Sci. 60: 623-624
  32. Sugawara Y. and Sakai A. 1974.Survival of suspension cultured sycamore cells, cooled to the temperature of liquid nitrogen. PlantPhysiol. 54: 722-724 https://doi.org/10.1104/pp.54.5.722
  33. Terumoto I. 1965. Freezing and drying in a red marnine alga, Porphyra yezoensis Ueda. LowTemp. Sci. Ser. B 23: 11-20 (in Japanese with English abstract)
  34. Tsuchiya H. 1989.Cryopreservation of free-living conchocelis of Porphyra yezoensis. Bull. Chiba Pref. Fish. Exp. Sta. 47: 35-36 (in Japanese)
  35. Tsuchiya H. 1992.Cryopreservation of free-living conchocelis of Porphyra yezoensis -II. Cryoprotectants and freezing method. Bull. Chiba Pref. Fish. Exp. Sta. 50: 37-43 (in Japanese)
  36. Tsuchiya H. 1994. Cryopreservation of free-living conchocelis of Porphyra yezoensis -III. Bull. Chiba Pref. Fish. Exp. Sta.52: 27-30 (in Japanese)
  37. Ulrich J.M., Finkle B.J., Moore P.H. and Ginoza H. 1979. Effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant. Cryobiology 16: 550-556 https://doi.org/10.1016/0011-2240(79)90073-7
  38. van der Meer J.P. and Simpson F.J. 1984. Cryopreservation of Gracilaria tikvahiae (Rhodophyta) and other macrophytic marine algae. Phycologia 23: 195-20 https://doi.org/10.2216/i0031-8884-23-2-195.1

피인용 문헌

  1. Cryopreservation of the gorgonian endosymbiont Symbiodinium vol.6, pp.1, 2016, https://doi.org/10.1038/srep18816
  2. Tissue Cryopreservation for Plant Conservation: Potential and Challenges vol.175, pp.1, 2014, https://doi.org/10.1086/673301
  3. Non-cryogenic preservation of thalli, germlings, and gametes of the green seaweed Ulva rigida vol.473, 2017, https://doi.org/10.1016/j.aquaculture.2017.02.012