Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Microsatellite DNA Polymorphism for Parentage Testing in Dog Breeds

개의 친자감정을 위한 Microsatellite DNA 다형성 분석

  • Cho, G. J. (Equine Blood Typing Laboratory, Korea Racing Association) ;
  • Cho, B. W. (Department of Animal Science, Miryang National University) ;
  • Kim, S. K. (Department of Animal Science, Miryang National University) ;
  • Lee, K. W. (Department of Animal Science, Miryang National University) ;
  • Kim, Y. K. (Genetic Institute, Pet child Co., Ltd)
  • 조길재 (한국마사회 혈액형검사실) ;
  • 조병욱 (밀양대학교 동물자원학과) ;
  • 김선구 (밀양대학교 동물자원학과) ;
  • 이길왕 (밀양대학교 동물자원학과) ;
  • 김영규 (펫챠일드(주) 유전자연구소)
  • Published : 2003.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate a usefulness of the microsatellite DNA markers for individual identification and parentage verification in three dog breeds. A total of 59 random dog (31 Chiwawa, 20 Poongsan, 8 Labrador Retriever) samples were genotyped by using 14 markers (Chiwawa dog), 16 markers (Poongsan dog), and 12 markers (Labrador Retriever dog) among the 17 international standard markers (PEZ1, 3, 5, 6, 8, 10, 11, 12, 13, 15, 16, 17, 20, 21, FHC2010, FHC2054 and FHC2079), respectively. The number of alleles per locus varied from 4 to 14 with a mean value of 6.07 in Chiwawa dog, 2 to 9 with a mean of 4.75 in Poongsan dog, and 3 to 5 with a mean of 4.00 in Labrador Retriever dog. Observed heterozygosity was ranged 0.419${\sim}$0.968 (mean 0.755), 0.300${\sim}$0.950 (mean 0.597) and 0.125${\sim}$0.750 (mean 0.604), and expected heterozygosity was ranged 0.432${\sim}$0.883 (mean 0.711), 0.262${\sim}$0.817 (mean 0.559) and 0.425${\sim}$0.808 (mean 0.660) in these three dog breeds. PIC value was ranged 0.397${\sim}$0.856 (mean 0.659), 0.222${\sim}$0.772 (mean 0.503) and 0.354${\sim}$0.717 (mean 0.563) in these three dog breeds. Of the 17 markers, PEZ1, PEZ3, PEZ6, PEZ10, PEZ12 loci, PEZ1, PEZ6, PEZ13 loci, and PEZ8, PEZ12 loci have relatively high PIC value (＞0.7) in Chiwawa dog, Poongsan dog and Labrador Retriever dog, respectively. The exclusion probability was ranged 0.240${\sim}$0.741, 0.111${\sim}$0.616, and 0.198${\sim}$0.529, and the combination of microsatellite loci was 0.9999, 0.9991, and 0.9968 in Chiwawa dog, Poongsan dog and Labrador Retriever dog, respectively. These results can give basic information for developing parentage verification and individual identification system in these three dog breeds.

국내에서 사육중인 치와와 31두, 풍산개 20두, 래브라도 리트리버 8두를 대상으로 micro-satellite DNA형의 유전자 빈도에 기초하여 heterozygosity, PIC, 그리고 PE를 분석한 결과를 요약하면 다음과 같다. 치와와의 대립유전자 수는 4${\sim}$14개로서 expected heterozygosity와 PIC는 각각 0.432${\sim}$0.883 (평균 0.711), 0.397${\sim}$0.856(평균 0.659)으로 나타났으며 PEZ1, PEZ3, PEZ6, PEZ10, PEZ12의 marker는 PIC 0.7이상으로 나타났고 14개 marker를 조합시 부권부정율은 0.9999로 관찰되었다. 풍산개의 대립유전자 수는 2${\sim}$9개로서 expected heterozygosity와 PIC는 각각 0.262${\sim}$0.817 (평균 0.559), 0.222${\sim}$0.772 (평균 0.503)으로 나타났고 PEZ1, PEZ6, PEZ13의 marker는 PIC 0.7이상으로서 16개 marker를 조합시 부권부정율은 0.9991로 관찰되었다. 래버라도 리트리버의 대립유전자 수는 3${\sim}$5개로서 expected heterozygosity와 PIC는 각각 0.425${\sim}$0.808(평균 0.660), 0.354${\sim}$0.717(평균 0.563)으로 나타났고 PEZ8, PEZ12의 marker는 PIC 0.7이상으로 관찰되었으며 12개 marker를 조합시 부권부정율은 0.9968로 관찰되었다. 이상의 결과는 microsatellite DNA형에 의한 개의 친자감정 및 개체식별에 유용한 자료로 활용할 수 있을 것으로 사료된다.

Keywords

References

  1. Cho, G. J., Yang, Y. J., Kang, H. S. and Cho, B. W. 2002. Genetic diversity and validation of microsatellite markers for Jeju native horse parentage testing. Korean Journal of Genetics. 24(4):359-365.
  2. Glowatzki-Mullis, M. L., Gaillard, C., Wigger, G. and Feies, R. 1995. Microsatellite-based parentage control in cattle. Animal Genetics. 26:7-12.
  3. Halverson, J. L. and Edwards, J. W. 2000. Microsatellite polymorphism in dog breeds-the AKC Parent Club study. Proc. 27th ISAG Conference on Animal Genetics. pp. 19.
  4. Han, S. K., Byun, H. D. and Chung, E. Y. 2000. Genetic diversity analysis and parentage control in Koreran native cattle using Microsatellite. Proc. 27th ISAG Conference on Animal Genetics. pp. 88.
  5. Jeffreys, A. J., Wilson, V. and Thein, S. L. 1985. Hypervariable minisatellite regions in human DNA. Nature. 314:67-73. https://doi.org/10.1038/314067a0
  6. Kim, K. S. and Choi, C. B. 2002. Genetic structure of Korean native pig using microsatellite markers. Korean Journal of Genetics. 24(1):1-7.
  7. Litt, M. and Luty, J. A. 1989. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American Journal of Human Genetics. 44:397-401.
  8. Marshall, T. C., Slate, J., Kruuk, L. and Pemberton, J. M. 1998. Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology. 7:639-655. https://doi.org/10.1046/j.1365-294x.1998.00374.x
  9. Polli, M., Marelli, S., Zanotti, M. and Guidobono Cavalchini, L. 2000. Polymorphism of micro- satellite loci and parentage identification in some Italian dog breeds. Proc. 27th ISAG Conference on Animal Genetics. pp. 45.
  10. Tozaki, T., Kakoi, H., Mashima, S., Hirota, K. I., Hasegawa, T., Ishida, N., Miura, N., Choi-Miura, N. H. and Tomita, M. 2001. Population Study and Validation of Paternity Testing for Thorough- bred Horses by 15 Microsatellite Loci. Journal of Veterinary Medicine Science. 63(11):1191-1197.
  11. 조길재. 2002. 더러브렛종 말의 Microsatellite DNA 다형. 한국유전학회지. 24(2):177-182.
  12. 채영진, 이병천, 이항. 1998. 유전자 감식에 의한 개에서의 친자감별. 한국임상수의학회지. 15(2): 274-278.
  13. 하지홍, 이성은, 탁연빈, 김종봉. 1998. 한국 토종개 집단의 형태특정과 혈액 단백질. 한국축산학회지. 40(6):711-720.
  14. 하지홍, 김경석. 1998. 한국 토종개의 기원에 관한 고찰. 한국축산학회지. 40(6):701-710.