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흰쥐에서 혈액지질 대사 및 항혈전작용에 관한 베타사이클로덱스트린의 생물학적 효과

The Biological Effects of β-Cyclodextrin on Antithrombotic Activity and Plasma Lipid Metabolism in Rats

  • 박병성 (강원대학교 동물생명과학전공)
  • Park, B. S. (Major of Animal Life Science, Kangwon National University)
  • 발행 : 2003.04.30

초록

일반사료, 고콜레스테롤사료 또는 포화지방함량이 높은 사료를 섭취한 흰쥐에서 항혈전작용과 혈액중성지방 및 콜레스테롤 수준에 관한 $\beta$-cyclodextrin($\beta$CD)의 급여효과를 조사하였다. 출혈시간은 일반사료, 고콜레스테롤 및 고지방사료 섭취군 모두에서 $\beta$CD 첨가구가 대조구에 비해서 각각 293%, 157% 및 218% 까지 유의적으로 연장되었다(p<0.05). 전혈응고시간은 일반사료, 고콜레스테롤 및 고지방사료섭취군에서 $\beta$CD 첨가구가 대조구와 비교할때 각각 202%, 168% 및 211%까지 유의적으로 높았다(p<0.05). $\beta$CD 첨가사료는 흰쥐 혈액내 총지질, 중성지방, 총콜레스테롤 및 저밀도지질단백질 콜레스테롤 함량을 현저하게 낮추는 원인이 되었다. 혈액내 총지질 함량은 일반사료, 고콜레스테롤 및 고지방사료 섭취군에서 $\beta$CD첨가구가 대조구에 비해서 각각 70%, 82% 및 87%까지 유의적으로 낮아졌다(p<0.05). 혈액내 중성지방함량은 일반사료, 고콜레스테롤 및 고지방사료 섭취군에서 $\beta$CD 첨가구가 대조구와 비교할 때 각각 89%, 43% 및 59%까지 유의적인 감소를 나타냈다(p<0.05). 혈액내 총콜레스테롤 함량은 일반사료, 고콜레스테롤 및 고지방사료 섭취군에서 $\beta$CD 첨가구가 대조구에 비해서 각각 28%, 62% 및 36%까지 유의적으로 낮아졌다(p<0.05). 혈액내 저밀도 지질단백질 콜레스테롤 함량은 일반사료, 고콜레스테롤 및 고지방사료섭취군에서 $\beta$CD 첨가구가 대조구에 비해서 각각 39%, 54% 및 25%까지 유의적인 감소를 나타냈다(p<0.05). 혈액내 총담즙산 함량은 일반사료, 고콜레스테롤 및 고지방사료섭취군에서 $\beta$CD 첨가구가 대조구에 비해서 각각 66%, 95% 및 97% 까지 유의적인 증가를 보였다(p<0.05). 일반사료 섭취군에서 $\beta$CD 첨가구의 HMG-CoA reductase 활성도는 대조구와 비교할 때 41%까지 유의적으로 낮아졌으며 분을 통한 스테로이드의 배설량은 167%까지 유의적인 증가를 나타냈다(p< 0.05). 이 결과는 $\beta$CD가 사람의 심장혈관계질환과 비만을 예방하는데 도움이 될 수 있는 혈액내 지질, 중성지방 및 콜레스테롤의 감소효과 그리고 항혈전작용에 관한 생리활성 기능을 갖는다는 것을 시사해 주고 있다.

The effect of feeding a cyclic oligosaccharide, $\beta$-cyclodextrin($\beta$CD) on plasma cholesterol and triacylglyceride concentrations and on antithrombotic activity were investigated in rats fed a control chow diet, or one either high in cholesterol or in saturated fat. The bleeding time of $\beta$CD-fed groups was significantly prolonged by 293%, 157% and 218% in normal, high cholesterol and high fat diet fed groups, respectively, as compared to the control group(p<0.05). The whole blood clotting time was significantly increased by 202%, 168% and 211% in normal, high cholesterol and high fat diet fed groups as compared to control group, respectively(p<0.05). The $\beta$CD diet caused a marked decrease in plasma total lipid(TL), triacylglyceride(TAG), total cholesterol (TC) and low density lipoprotein- cholesterol (LDL-C) concentrations. The plasma TL concentration was significantly decreased by 70%, 82% and 87% in normal, high cholesterol and high fat diet fed groups as compared to the control group, respectively(p<0.05). The plasma TAG concentration was significantly decreased by 89%, 43% and 59% in normal, high cholesterol and high fat diet fed groups, respectively, as compared to the control group(p<0.05). The plasma TC concentration was significantly decreased by 28%, 62% and 36% in normal, high cholesterol and high fat diet fed groups, respectively, as compared to the control group(p<0.05). The LDL-C concentration was significantly decreased by 39%, 54% and 25% in normal, high cholesterol and high fat diet fed groups as compared to control group, respectively(p<0.05). The plasma total bile acids contents of $\beta$CD group was significantly increased by 66%, 95% and 97% in normal, high cholesterol and high fat diet fed groups as compared to control group, respectively(p<0.05). The hepatic HMG-CoA reductase activity was significantly lowered by 41% in the $\beta$CD-fed group compared to normal diet fed rats(p<0.05). The fecal steroid excretions of the $\beta$CD groups was significantly increased by 167% in normal diet fed rats(p<0.05). These results suggest that the $\beta$CD has a biological active function on antithrombotic activity and is hypolipidemic, hypotriglyceridemic and hypocholesterolimic agents. These are all effects that can help to prevent obesity and coronary heart disease in humans.

키워드

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피인용 문헌

  1. The Effect of Antifat Diets with β-Cyclodextrin on the Weight Loss in Obese Korean Women vol.33, pp.5, 2004, https://doi.org/10.3746/jkfn.2004.33.5.832