한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제26권3호
  • /
  • Pages.199-202
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  • 2003
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  • 3022-7372(pISSN)
  • /
  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

테트라싸이클린계 잔류항생물질 분석을 위한 전처리방법 비교

Comparison of pretreatment methods for tetracyclines analysis by HPLC

  • 김동언 (서울특별시보건환경연구원) ;
  • 황래홍 (서울특별시보건환경연구원) ;
  • 윤은선 (서울특별시보건환경연구원) ;
  • 함희진 (서울특별시보건환경연구원) ;
  • 양윤모 (서울특별시보건환경연구원) ;
  • 김창기 (서울특별시보건환경연구원) ;
  • 기노준 (서울특별시보건환경연구원) ;
  • 이정학 (서울특별시보건환경연구원)
  • Kim, Dong-Eon (Seoul Metropolitan Health & Environment Research Institute) ;
  • Hwang, Lae-Hwong (Seoul Metropolitan Health & Environment Research Institute) ;
  • Yun, En-Sun (Seoul Metropolitan Health & Environment Research Institute) ;
  • Ham, Hee-Jin (Seoul Metropolitan Health & Environment Research Institute) ;
  • Yang, Yoon-Mo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim, Chang-Gi (Seoul Metropolitan Health & Environment Research Institute) ;
  • Ki, No-Jun (Seoul Metropolitan Health & Environment Research Institute) ;
  • Lee, Jung-Hark (Seoul Metropolitan Health & Environment Research Institute)
  • 발행 : 2003.10.01
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초록

Solid phase extraction(SPE) and matrix solid phase dispersion(MSPD) have been studied as preparation procedures for tetracyclines analysis by high-performance liquid chromatography(HPLC) in meat. The recovery range was 74${\sim}$98% for SPE, and 72${\sim}$93% for MSPD at spiked levels of 100ng/g for oxytetracycline(OTC), tetracycline(TC), chlortetracycline(CTC), and doxycycline(DC). The detection limits were 15${\sim}$78ng/g for SPE and 25${\sim}$84ng/g for MSPD, respectively. Analytical method was HPLC with UV detector. The purpose of this study was developing a practical, accurate and precise method for rapid extraction and quantitation of tetracycline residues in meat.

키워드

참고문헌

  1. Nicholas HB, Mcdonald LE. 1985. Veterinary Pharmacology and Therapeutics. 6 ed. Iowa State University Press: 813-821.
  2. Thomas MH. 1989. Simultaneous determination of oxytetracycline, tetracycline, and chlortetracycline in milk by liquid chromatography. JAOAC Int 72:564-567
  3. Noben, JP, Missotten M, Hendriks L, et al. 1996. Determination of oxytetracycline in kidney and diaphragm. Proc. Euroresidue III Veldhooen : 760-764
  4. Farrington WH, Tarbin J. Bygrave J. et al. 1991. Analysis of trace residues of tetracyclines in animal tissues and fluids using metal chelate affinity chromatography/HPLC. Food Additives Contaminants 8:55-64
  5. Frgalova K, Hera A, Valova J. 1996. Determination of veal calves by high performance liquid chromatography and ELISA. Proc. Euroresidue III Veldhoven: 407-410
  6. Michel M. 1970. Analysis of antibiotics by gas chromatography II . J chromatography 47:341-347 https://doi.org/10.1016/0021-9673(70)80050-4