도축우에서 Mannheimia haemolytica의 분리 및 항생제 감수성검사

Isolation and antimicrobial susceptibility test of Mannheimia haemolytica from slaughtered cattle

  • 변재원 (인천광역시보건환경연구원) ;
  • 김경호 (인천광역시보건환경연구원) ;
  • 이승환 (인천광역시보건환경연구원) ;
  • 이혜화 (인천광역시보건환경연구원) ;
  • 이정인 (인천광역시보건환경연구원) ;
  • 황현순 (인천광역시보건환경연구원)
  • Byun, Jae-Won (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Kim, Kyoung-Ho (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Lee, Seoung-Whan (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Lee, Hye-Wha (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Lee, Jung-In (Incheon Metropolitan Health & Environmental Research Institute) ;
  • Hwang, Hyun-Soon (Incheon Metropolitan Health & Environmental Research Institute)
  • 발행 : 2003.06.01

초록

This study was carried out to identify and investigate antimicrobial susceptibility for Mannheimuia haemolytical which is responsible for shipping fever. Samples were collected from nasal and lung of 100 adult healthy cattle which are slaughtered in Samsung meat corporation located in Incheon metropolitan city. lung lesion index have been investigated within 0-5 range according to Shewen and Willkie(Can J Vet Res 52:30-36, 1988). Eighty-seven of 100 cattle were under normal condition with 0-1 ranges. A total of 129 strains were collected from blood and tryptic soy agar. Among these strains, 100 strains were identified with Staphylococcus, Streptococcus and enterobacteria containing E coli. Biochemical and fermentation assay of arabinose, trehalose, xylose, mannose, mannitol, lactose and salicin were tested to identify with Mannheimia sp. for 7 strains shown haemolytic activity on blood agar. Five strains were identified with Mannheimia haemolytica and 2 strains were untyped. In seasonal survey, Mannheimia sp recovered from fall to winter(5 of 7) have been highly isolated rather than those from spring to summer(2 of 7). Mannheimiz haemolytica were susceptible to antibacterials tested in this study but more resistant to oxytetracycline and streptomycin.

키워드

참고문헌

  1. Yoo HS. 1995. Effects of Pasteurella haemolytica Al lipopolysaccaride and leukotoxin on bovine alveolar macrophages. PhD thesis in Minnesota University : 1-3
  2. Mannheim W. 1984. Pasteurellaceae. In: Krieg NR(Eds). Bergey's manual of systemic bacteriology Vol 1. William & Willkins. Baltimore: 550-558
  3. Frank GH, Briggs RE, Loan RW, et al. 1996. Respiratory tract disease and mucosal colonization by Pasteurella haemolytica. Am J Vet Res 57:1317-1320
  4. Wilkie BN, Shewen PE. 1988. Defining the role that Pasteurella baemolytica plays in shipping fever. Vet Med Symposium: 1053-1058
  5. Whiteley LO, Maheswaran SK, Weiss DJ, et al. 1992. Pasteurella haemolytica Al and bovine respiratory disease; pathogenesis. J Vet Int Med 6:11-22
  6. Angens O, Quirie M, Donachie W, et al. 1999. Investigations on the species specificity of Mannheimia(Pasteurella) haemolytitica serotyping, Vet Microbiol 65:283-290
  7. Angens O, Ahrens P, Bisgaard M. 2002. Phenotypic and genotypic characterization of Mannheimia(Pasteurella) haemolyticalike strains isolated diseased animals in Denmark. Vet Microbiol 84:103-114 https://doi.org/10.1016/S0378-1135(01)00439-4
  8. Confer AW, McCraw RD, Durham JA. et al. 1995. Serum antibody responses of cattle to iron-regulated outer-membrane proteins of Pasteurella haemolytica A1. Vet Immunol Immunopathol 47:101-110 https://doi.org/10.1016/0165-2427(94)05390-E
  9. Shewen PE, Wilkie BN. 1988. Vaccination of calves with leukotoxic culture supernatant from Pasteurella haemolytica. Can J Vet Res 52:30-36
  10. Conlon JAR, Shewen PE 1993. Clinical and serological evaluation of a Pasteurella haemolytica A1 capsular polysaccharide vaccine. Vaccine 11:767-772 https://doi.org/10.1016/0264-410X(93)90263-W
  11. Morck DW, Raybould TJG, Acres SD, et. al. 1987. Electron microscopic description of glycocalyx and fimbriae on the surface of Pasteurella haemolytica A1. Can J Vet Res 51:83-88
  12. Frank GH, Tabatabai LB. 1981. Neuraminidase activity of Pasteurella haemolytica. Infect Immun 32:1119-1122
  13. 조광현, 김봉환. 1991. 호흡기증상을 나타낸 송아지 및 산양에서 분리한 Pasteurella haemolytica의 생화학적 특성 및 약제감수성. 대한수의학회지 31(4):449-456
  14. Quinn PJ, Carter GR. 1994. Clinical veterinary bacteriology. Mosby, Wolfe publishing. London: 254-258
  15. Biberstein EL. 1978. Biotyping and serotyping of Pasteurella haemolytica. In: Bergen T, Norris JR(Eds). Methods in Microbiology. Academic press, London: 253-269
  16. Reeve-Johnson L. 2001. Relationships between clinical and pathological signs of disease in calves infected with Mannheimia(Pasteurella) haemolytica type A1. Vet Rec 149:549-552
  17. Frank GH, Briggs RE, Gillette KG 1987. Pasteurella haemolytica serotype 1 colonization of nasal passages of virusinfecte calves. Am J Vet Res 48:1674-1677
  18. Confer AW, Corsvet RE, Panciera RJ, et al. 1983. Isolation of Pasteurella haemolytica and correlation with serum antibody response in clinically normal beef calves. Vet Microbiol 8:601-610 https://doi.org/10.1016/0378-1135(83)90008-1
  19. Confer AW, Fluton RW, Clinkenbeard KD, et al. 1998. Duration of serum antibody responses following vaccination and revaccination of cattle with nonliving commercial Pasteurella haemolytica vaccines. Vaccine 16(20):1962-1970 https://doi.org/10.1016/S0264-410X(98)00115-7
  20. Shewen PE. 1995. Host response to infection with HAP. Donachie W, Lainson FA, Hudgson Jc(Eds). Plenum, London: 165-171
  21. DeROSA DC, Mechor GD, Staats JJ, et al. 2000. Comparison of Pasteurella spp. simultaneously isolated from nasal and transtracheal swabs from cattle with clinical signs of bovine respiratory disease. J Clin Microbiol 38(1):327-332
  22. Allan EM, Wiseman A, Gibbs HA. 1985. Pasteurella species isolated from the bovine respiratory tracts and their antimicrobial sensitivity patterns. Vet Rec 117:629-631
  23. Guilmour NJL, Sharp JM, Guilmour JS. 1982. Effect of oxytetracycline therapy on experimentally induced pneumonic pasteurellosis in lambs. Vet Rec 108: 97-99