Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Chinese yam necrotic mosaic virus Isolated from Chinese Yam in Korea

한국산 장마(Dioscorea oppasita cv. Jang-Ma)에서 분리한 Chinese yam necrotic mosaic virus

  • 강동균 (경상북도 농업기술원 생물자원연구소) ;
  • 곤도토루 (아오모리 그린바이오센터) ;
  • 신종희 (경상북도 농업기술원 생물자원연구소) ;
  • 신혜영 (영남대학교 이과대학 생물학과) ;
  • 성정현 (영남대학교 이과대학 생물학과) ;
  • 강상구 (영남대학교 생명공학연구소) ;
  • 장무웅 (영남대학교 이과대학 생물학과)
  • Published : 2003.09.01
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Abstract

Chinese yam (Dioscorea opposita cv. Jang-Ma) plants showing necrotic mosaic symptom were collected from their growing fields in Andong, Euisong, Gunwi and Daegu, Korea. Direct negative stainning method by electron microscope showed filamentous particles of about 660 nm in length. Immunosorbent electron microscopy (ISEM) revealed filamentous particles of 660nm decorated with antiserum of Chinese yam necrotic mosaic virus (ChYNMV). The virues purified partially were used to isolate viral RNA as template for RT-PCR to amplify about 1.2 kbp of 3'-terminal region (coat protein, 3'-UTR) with ChYNMV specific and oligo-dT primers. Amino acids sequences of amplified CP genes revealed that the viruses shared 97.9% similarity with ChYNMV (AB044386) wh ich was known as the member of Macluravirus. So the viruses from Chinese yam (D. opposita cv. Jang-Ma) plants were identified as ChYNMV. Comparing the CP amion acid sequences of ChYNMV strains with other macluraviruses such as Cardamon mosaic virus (CdMV), Narcissus latent virus (NLV) and Maclura mosaic virus (MacMV) revealed that N-terminal was the most varialbe region and conserved regions were present within the genus Macluravirus.

경상북도 안동, 의성, 군위, 대구의 장마 재배포장에서 모자이크와 괴저반점을 보이는 장마잎을 채집하였다. 이들 이병 잎 조직을 시료로 하여 D N 법 및 ISEM법에 의해 투과전자현미경으로 바이러스 입자를 관찰한 결과, 각각의 시료에서 ChYNMV 항혈청에 반응한 660nm의 사상형 입자가 확인되었다. 장마 잎에서 부분 정제한 바이러스에서 RNA를 추출하여 이것을 주형으로 ChYNMV 특이적 프라이머와 oligo-dT 프라이머를 이용하여 외피단백질 유전자와 3‘-말단 비전사부위를 포함하는 약 1.2kbp의 3’-말단을 증폭하였다. 외피단백질 아미노산서열은 Macluravirus로 알려진 ChYNMV (A B044386)와 97.9%의 상동성을 보여 장마에서 분리한 바이러스를 ChYNMV로 동정하였다. ChYNMV와 다른 Macluavirus의 외피 단백질 아미노산서열을 비교한 결과, M말단 영역에서 가장 많은 변이를 확인하였고 Macluravirus 속에는 잘 보존된 영역이 존재하였다.

Keywords

References

  1. Aleman, M. E., Marcos, J. F., Brugidou, C., Beachy, R. N. and Fauquet, C. 1996. The complete nucleotide sequence of yam mosaic virus (Ivory Coast isolate) genomic RNA. Archives of virology 141: 1259-1278 https://doi.org/10.1007/BF01718829
  2. Badge, J., Robinson, D. J., Brunt, A. A. and Foster, G. D. 1997.3'Terminal sequences of the RNA genomes of narcissus latent and Maclura mosaic viruses suggest that they represent a new genus of the Potyviridae. J. Gen. Virol. 78: 253-257 https://doi.org/10.1099/0022-1317-78-1-253
  3. Badge, J., Robinson, D. J., Brunt, A. A. and Foster, G. D. 1997.3'Terminal sequences of the RNA genomes of narcissus latent and Maclura mosaic viruses suggest that they represent a new genus of the Potyviridae. J. Gen. Virol. 78: 253-257 https://doi.org/10.1099/0022-1317-78-1-253
  4. Domier, L. L., Franklin, K. M., Shahabuddin, M., Hellmann, G. M., Overmeyer, J. H., Hiremath, S. T., Siaw, M. F. E., Lomonossoff, G. P., Shaw, J. G. and Rhoads, R. E. 1986. The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acid Res. 13: 5417-5430
  5. Fukumoto, F. and Tochihara, H. 1978. Chinese yam necrotic mosaic virus. Ann. Phytopath. Soc. Japan 44: 1-5 https://doi.org/10.3186/jjphytopath.44.1
  6. Jacob, T. and Usha, R. 2001. 3'-Terminal sequence analysis of the RNA genome of the Indian isolate of cardamom mosaic virus: a new member of genus Macluravirus of Potyviridae. Virus Genes 23: 81-88 https://doi.org/10.1023/A:1011191614839
  7. 石川 亮, 難波成任, 山下修一, 土居養二. 1985. Broad bean wilt virus (BBWV)の純化および理化學的性狀について. Ann. Phytopathol. Soc. Jpn. 51(3): 352-353.
  8. Kondo, T. 2000. Detection of Chinese yam necrotic mosaic virus by RT-PCR. Jpn. J. Phytopathol. 66: 263 https://doi.org/10.3186/jjphytopath.66.263
  9. Kondo, T. 2001. The 3'-terminal sequence of Chinese yam necrotic mosaic virus genomic RNA: a close relationship with Macluravirus. Arch. Virol. 146: 1527-1535 https://doi.org/10.1007/s007050170076
  10. Kondo, T, Sasaki, Y., Fuji, S., Kang, D.-K. and Chang, M.-U. (2002). Occurrence of Broad bean wilt virus 2 in Yams. Jpn. J. Phytopath. 68: 239 (Abstr. in Japanese)
  11. Matthews, R.E.F. 1991. Plant virology. 3rd ed. Academic Press. San Diego
  12. Rodriguez-Cerezo, E., Gamble-Klein, P. and Shaw, J. G. 1991. A determinant of disease symptom severity is located in the 3'terminal noncoding region of the RNA of a plant virus. Proc. natl. Acad. Sci. USA 88: 9863-9867 https://doi.org/10.1073/pnas.88.21.9863
  13. Shirako, Y. and Ehara, Y. 1986. Rapid diagnosis of Chinese yam necrotic mosaic virus infection by electro-blot immunoassay. Ann. Phytopath. Soc. Japan 52: 453-459 https://doi.org/10.3186/jjphytopath.52.453
  14. Tochihara, H. 1993. Yams. In: Tsuchizaki, T., Tochihara, H., Kameya, M., Yanase, H. (eds) Plant virus diseases in Japan. Zenkoku Noson Kyokai, Tokyo pp. 431-435
  15. Turpen, T. 1989. Molecular cloning of a Potato virus Y genome: nucleotide sequence homology in non-coding region of Potyviruses. J. Gen. Virol. 70: 1951-1960 https://doi.org/10.1099/0022-1317-70-8-1951

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