Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Cytotoxic Activity of the Extracts from Curcuma zedoaria

  • Kim, Myoun-Gae (Department of Food and Bioengineering, Dongseo University) ;
  • Kim, Jung-Sun (Department of Food and Bioengineering, Dongseo University) ;
  • Hong, Jon-Ki (Korea Baic Science Institute) ;
  • Ji, Ming-Jie (Life Science School, Sandong University China) ;
  • Lee, Yong-Kyu (Department of Food and Bioengineering, Dongseo University)
  • Published : 2003.12.01
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Abstract

The effect of the hexane extract of Curcuma zedoaria roots and its solvent fractions were investigated on the proliferation of SiHa, SNU-1 and HepG2 cell lines. Among those fractions, final fraction H2-3-1 and H2-3-3 showed cytotoxic effect on SiHa and HepG2 cell lines. The hallmark of apoptosis, DNA fragmentation, also appeared in the final fractions H2-3-1 and H2-3-3 after 24h treatment in SiHa cell line. Furthermore, those fractions were shown to be able to induce cell death in $[^3H]$thymidine incorporation test. These two fractions, H2-3-1 and H2-3-3 were determined as (-)-$\alpha$-curcumene and $\beta$-tumerone respectively by NMR and mass spectrum. From these results, it is speculated that te hexane extract of Curcuma zedoaria is necessary for further studies as a potent inhibitor of the growth of cancer cells.

Keywords

References

  1. Ewa, S., Anna, S.Z., Katarzyna, P., Janusz, S. and Ewa, R. (1997): Inhibition of proliferation and apoptosis of human and rat T lymphocytes by curcumin, a curry pigment. Biochem. Pharmacol., 54, 899-907
  2. Han, D. (1998): Pharmacognosy, Dong Myung Sa, Seoul, pp. 155-156
  3. Im, E.O., Lee, S., Suh, H., Kim, K.W., Bae, Y.T. and Kim, N.D. (1999): A novel ursodeoxycholic acid derivative induced apoptosis in MCF-7 human breast cancer cells. Pharm. Pharmacol. Commun., 5,1-7
  4. Kang, Y.H., Kim, B.W., Ha, Y.M., Park, J.K., Nan, S.Y., Choi, K.C. and Choi, Y.M. (1987): Experimental studies on antitumor activity of herb drugs(I). Kor. J. Pharmacogn., 18, 118-126
  5. Matsuda, H., Ninomiya, K., Morikawa, T. and Yoshikawa, M. (1998): Inhibitory effect and action mechanism of sesquiterpenes from Zedoaria rhizoma on D-galactosamine/lipopolysaccharide-induced liver injury. 8ioorg. Med. Chem. Lett., 8, 339-344
  6. Moon, C.K., Park, K.S., Lee, S.H. and Yoon, Y.P. (1985): Antitumor activities of several phytopolysaccharides. Arch Pharm Res., 8, 42-44
  7. Mossman, T. (1983): Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immuno. Methods., 65, 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  8. Lee, H. and Lin, J.Y. (1988): Antimutagenic activity of extracts from anticancer drugs Chinese medicine. Mutat Res., 204, 229-234
  9. Rao, B.G. and Nigam, S.S. (1970): Antimicrobial activity of Curcuma zedoaria, Indian J. Med. Res., 58, 627-633
  10. Shin, K.H., Kim, O.N. and Woo, W.S. (1989): Isolation of hepatic drug metabolism inhibitors from the rhizomes of Curcuma zedoaria. Arch. Pharm. Res., 12, 196-200
  11. Steller, H. (1995): Mechanism and genes of cellular sucide. Science., 267, 1445-1448
  12. Syu, W.J., Shen, C.C., Don, M.J., au, J.H., Lee, G.H. and Sun, D.M. (1998): Cytotoxicity of curcuminoids and some novel compounds from Curcuma zedoaria. J. Natl. Prod., 61, 1531-1534 https://doi.org/10.1021/np980269k
  13. Tradimed. (1996): Natural product research institute
  14. Yoshinori, S., Yoshinori, A., Mitsuaki, K., Koji, Y. and Tsunematsu, T. (1985): Curcumenone, curcumanolide A and curcumanolide B, three sesquiterpenoids from Curcuma zedoaria. Phytochemistry., 24, 2629-2633
  15. Yoshioka, T., Fujii, E., Endo, M., Wada, K., Tokunaga, Y., Shiba, N., Hohsho, H., Shibuya, H. and Muraki, T (1998): Antiinflammatory potency of dehydrocurdione, a zedoaryderived sesquiterpene. Inflammation Res., 47, 476-481