Korean Journal of Agricultural and Forest Meteorology (한국농림기상학회지)

Korean Society of Agricultural and Forest Meteorology (한국농림기상학회)
권호 표지

Physiological Tolerance of Native Tree Species in Abandoned Coal Mine Spoils

탄광 폐석지내 자생 수종의 생리적 피해 및 내성

  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to assess the physiological tolerance of native tree species for successful restoration and revegetation of abandoned coal-mine spoils. Study sites were two coal-mine spoils (Sododong and Ssarijae) in Taebaek, Kangwon Province, Korea. Five individuals of Betula costata and of B. schmidtii were analyzed for malondialdehyde (MDA) and hydrogen peroxide ($H_2O$$_2$) content, nitrate reductase (NR) and superoxide dismutase (SOD) activity, and for carbohydrate concentration in the leaves. Trees in the abandoned coal-mine spoils were influenced by deficiencies expressed by MDA and $H_2O$$_2$ content in the leaves of two species being higher at the coal-mine spoils than in the surrounding forest. Low NR activity indirectly represented nitrogen deficiency in the soil of the coal-mine spoils; an unmanageable SOD activity implied that tolerant functions didn't net against a certain stress of the coal-mine spoils. Decreased glucose and increased starch concentration especially showed the inhibition of the carbohydrate metabolism by inadequate factors. Consequently, low nitrogen content in the real-mine soils might increase damage in trees as a result of inhibiting the expression of tolerance mechanisms against stress. Therefore, trees in coal-mine spoils need ample nitrogen to use as a metabolic energy source in order to prevent damage and increase tolerance against stress.

본 연구는 폐석지의 성공적인 식생 복원을 위하여 자생 수종을 대상으로 피해 수준을 평가하고, 내성 특성을 구명하고자 실시하였다. 연구 조사지는 강원도 태백시 소도동과 싸리재에 위치한 폐석지를 대상으로 하였으며, 이미 선발한 거제수나무와 박달나무의 잎을 채취하여 NR 활성, MDA와 $H_2O$$_2$ 함량, SOD 활성, 탄수화물 함량을 분석하였다. 폐석지 내 수목들은 폐석지 주변 산림 내 수목들 보다 MDA함량과 $H_2O$$_2$ 함량이 높아 폐석지 내 부적당한 환경 요인에 영향을 받고 있음을 보여 주었다. 또한 낮은 NR 활성은 폐석지 내 질소원의 부족을 간접적으로 나타냈으며, 폐석지내 수목의 잎에서 항산화 효소인 SOD의 활성 증가가 나타나지 않은 것으로 보아 스트레스에 대한 내성 기능이 적절하게 작용하지 못하고 있음을 알 수 있었다. 특히 포도당의 감소와 전분의 증가는 탄수화물 대사가 부적당한 환경 요인에 의해 저해되고 있음을 보여 주었다. 결론적으로 폐석지 토양 내 낮은 질소함량은 스트레스에 대한 수목의 내성 발현에 도움을 주지 못하여 수목의 피해가 증가한 것으로 판단된다. 따라서 폐석지 내 수목의 피해를 막고 스트레스에 대한 내성을 증가시키기 위해서는 각종 대사의 에너지원으로 이용되는 양료 공급이 절실히 요구된다.

Keywords

References

  1. Adams, M. A. and P. M. Attiwill, 1982: Nitrogen mineralization and nitrate reduction in forests. Soil Biology and Biochemistry, 14, 197-202.
  2. Balsberg-$P{\aa}hlsson$, A. M., 1989: Effects of heavy-metal and $SO_2$ pollution on the concentrations of carbohydrates and nitrogen in tree leaves. Canadian Journal of Botany, 67, 2106-2113.
  3. Beauchamp, C. and I. Fridovichi, 1971: Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276-297.
  4. Bernt, E. and H. U. Bergmeyer, 1974: Inorganic peroxides. Methods of Enzymatic Analysis, Vol 4. (Eds.) H. U. Bergmeyer, Academic Press, New York, 2246-2248.
  5. Cadenas, E., 1989: Biochemistry of oxygen toxicity. Annual Review of Biochemistry, 58, 79-110.
  6. Crick, J. C. and J. P. Grime, 1987: Morphological plasticity and mineral nutrient capture in two herbaceous species of contrasted ecology. New Phytologist, 109, 403-414.
  7. Davis, D. G. and H. R. Swanson, 2001: Activity of stressrelated enzymes in the perennial weed leafy spurge (Euphorbia esula L.). Environmental and Experimental Botany, 46, 95-108.
  8. Ericsson, A., 1979: Effects of fertilization and irrigation on the seasonal changes of carbohydrate reserves in different age-class of needle on 20-year-old Scots pine(Pinus silverstris). Physiologia Plantarum, 45, 270-280.
  9. Esterbauer, H. and K. H. Cheeseman, 1990: Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in Enzymology, 186, 407-421.
  10. Foyer, C. H., 1993: Ascorbic acid, Antioxidant in Higher Plants. (Eds.) R. G. Alscher and J. C. Hess, CRC Press, 31-58.
  11. Han, S.-H., J.-C. Lee, S.-S. Jang and Y.-P. Hong, 2002: Comparison of the ozone sensitivity of four Betula species based on carbon allocation and partitioning patterns. Journal of Korean Forest Society, 91, 449-456.
  12. Hendry, G. A. F. and A. H. Price, 1993: Stress indicators : chlorophylls and carotenoids. Methods in Comparative Plant Ecology : A Laboratory Manual. G.A.F. Hendry and J.P. Grime, (Eds.) Chapman & Hall, 148-152.
  13. Hossner, L. R. and F. M. Hons, 1992: Reclamation of mine tailings. Advances in Soil Science. Vol. 17. (Eds.) B. A. Stewart. Springer-Verlag, New York, 311-348.
  14. Högberg, P., A. Granstrom, T. Johansson, A. Lundmark- Thelin and T. Näsholm, 1986: Plant nitrate reductase activity as an indicator of availability of nitrate in forest soils. Canadian Journal of Forest Research, 16, 1165- 1169.
  15. Kim, K.-H., H.-Y. Kim and B.-H. Kim, 2000: Development of nature-oriented technology for revegetation of abandoned coal-mine lands - In case study of revegetation experiment in the abandoned coal-mine spoils of Taebaek -. International Symposium for the Development of Environmental Restoration and Revegetation Technology in the Abandoned Coal-Mine Lands. 133-161.
  16. Kim, M. H. and S. W. Lee, 1994: Injury responses of landscape woody plants to air pollutants - malondialdehyde content. Journal of Korean Forest Society, 83, 25-31.
  17. Kim, N.-C. and S.-J. Nam, 2000: Ecological restoration measure in the coal mine lands - In case study of the revegetation works by natural topsoil restoration methods for the restoration of the abandoned coal-mine spoils -. International Symposium for the Development of Environmental Restoration and Revegetation Technology in the Abandoned Coal-Mine Lands. 162-188.
  18. Lee, J.-C., S.-H. Han, S.-S. Jang, J.-H. Lee, P.-G. Kim, J.- S. Hur and K.-J. Yum, 2002: Selection of indigenous tree species for the revegetation of the abandoned coal mine lands in Taeback Area. Korean Journal of Agricultural and Forest Meteorology, 4, 86-94.
  19. Lee, J.-C., S.-H. Han, S.-S. Jang, K.-J. Cho and Y.-Y. Kim, 2002: Effects of ozone uptake rate on photosynthesis and antioxidant activity in the leaves of Betula species. Korean Journal of Agricultural and Forest Meteorology, 4, 72-79.
  20. Logan, T. J., 1992: Chemical degradation of soil. Advances in Soil Science. Vol. 17. (Eds.) B. A. Stewart, Springer-Verlag, New York, 13-35.
  21. Prasad, T. K., M. D. Anderson, B. A. Martin and C. R. Stewart, 1994: Evidence for chilling-induced oxidative stress in maize seedlings and a regulator role for hydrogen peroxide. Plant Cell, 6, 65-74.
  22. Robinson, J. M. and R. A. Rowland, 1996: Carbohydrate and carbon metabolite accumulation responses in leaves of ozone tolerant and ozone susceptible spinach plants after acute ozone exposure. Photosynthesis Research, 50, 103-115.
  23. Samarakoon, A. B. and W. E. Rauser, 1979: Carbohydrate levels and photoassimilate export from leaves of Phaseolus vullgaris exposed to cobalt, nickel and zinc. Plant Physiology, 63, 1165-1169.
  24. Srivastava, H. S., 1980: Regulation of nitrate reductase activity in higher plants. Phytochemistry, 19, 725-733.
  25. Voeller, P. J., B. A. Zamora and J. Harsh, 1998: Growth response of native shrubs to acid mine spoil and to proposed soil amendments. Plant and Soil, 198, 209-217.
  26. Woo, B.-M. 2000: Evaluation for rehabilitation countermeasures of coal-mined spoils and denuded lands. Journal of the Korean Society for Environmental Restoration and Revegetation Technology, 3, 24-34.
  27. Yum, K.-J., P.-G. Kim and E. W. Park, 1999: Effects of sewage sludge application for restoration of abandoned mine areas. Journal of Korean Society of Environmental Engineers, 21, 2329-2340.