Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Molecular Differentiation of Panax Species by RAPD Analysis

  • Published : 2003.08.01
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Abstract

Traditional taxonomic methods used for the identification and differentiation of ginsengs rely primarily on morphological observations or physiochemical methods, which cannot be used efficiently when only powdered forms or shredded material is available. Randomly amplified polymorphic DNA (RAPD) was used to determine the unique DNA profiles that are characteristic not only of the genus Panax but also of various Panax subgroups collected from five different countries. RAPD results of OP-5A primer showed a specific single band that is characteristic of all ginseng samples. RAPD results of OP-13B primer demonstrated that OP-13B primer could be used as a unique RAPD marker to differentiate Panax species. These results support that this approach could be applied to distinguish Korean Ginseng (Panax ginseng) from others at the molecular level.

Keywords

References

  1. Chung, Y. Y., Chung, C. M., Ko, S. R., and Choi, K. T., Comparison of Agronomic Characteristics and Chemical Component of Panax ginseng C.A. Meyer and Panax quinquefolium L. Korean J. Ginseng Sci., 19(2), 160-164 (1995)
  2. Fuzzati, N., Gabetta, B., Jayakar, K., Pace, R., and Peterlongo, F., Liquid Chromatography-Electrospray Mass spectrometric Identification of ginsenosides in Panax ginseng roots. J Chromatogr A., 854(1-2), 69-79 (1999) https://doi.org/10.1016/S0021-9673(99)00463-X
  3. Ha, W. Y., Yau, F. C., But, P. P., Wang, J., and Shaw, P. C., Direct amplification of Length Polymorphism Analysis differentiates Panax ginseng from P. quinquefolius. Planta Med., 67(6), 587-589 (2001) https://doi.org/10.1055/s-2001-16483
  4. Jeong, C. S., Effect of Butanol Fraction of Panax ginseng head on Gastric lesion and Ulcer. Arch. Pharm. Res., 25(1), 61-66 (2002) https://doi.org/10.1007/BF02975263
  5. Ji, Q. C., Harkey, M. R., Henderson, G. L., Gershwin, M. E., Stern, J. S., and Hackman, R. M., Quantitative Determination of Ginsenosides by High-Performance Liquid Chromatography-tandem mass spectrometry. Phytochem. Anal., 12(5), 320-326 (2001) https://doi.org/10.1002/pca.593
  6. Ko, J. G., Kim, Y. S., Kim, H. Y., Na, K. C., Do, J. H., Park, J. D., Park, J. K., Park, H. J., Baek, N. I., Lee, S. J., Lee, J. H., Lee, H. O., and Jeong, K. T., Korean Ginseng. Korea Ginseng & Tobacco Research Institute (1994)
  7. Ko, S.-R., Choi, K.-J., and Han, K.-W., Comparison of Proximate Composition, Mineral Nutrient, Amino Acid and Free Sugar Contents of Several Panax Species. Korean J. Ginseng Sci., 20(1), 36-41 (1996)
  8. Li, W., Gu, C., Zhang, H., Awang, D. V., Fitzloff, J. F., Fong, H. H., and van Breemen, R. B., Use of High-Performance Liquid Chromatography-tandem mass Spectrometry to distinguish Panax ginseng C. A. Meyer (Asian ginseng) and Panax quinquefolius L. (North American ginseng). Anal. Chem., 72(21), 5417-5422 (2000) https://doi.org/10.1021/ac000650l
  9. Ngan, F., Shaw, P., But, P., and Wang, J., Molecular Authentication of Panax species. Phytochemistry, 50, 787-791 (1999) https://doi.org/10.1016/S0031-9422(98)00606-2
  10. Oiki, S., Kawahara, T., Inoue, K., Ohara, M., and Maki, M., Random Amplified Polymorphic DNA (RAPD) Variation among Populations of the Insular Endemic Plant Campanular microdonta (Campanulceae). Annals of Botany., 87, 661-667 (2001) https://doi.org/10.1006/anbo.2001.1389
  11. Shaw, P. C. and But, P. P., Authentication of Panax species and their adulterants by Random-Primed Polymerase Chain Reaction. Planta Med., 61(5), 466-469 (1995) https://doi.org/10.1055/s-2006-958138
  12. Tochika-Komatsu, Y., Asaka, I., and Ii, I., A random amplified polymorphic DNA (RAPD) primer to assist the Identification of a selected strain, aizu K-111 of Panax ginseng and the Sequence amplified. Biol. Pharm. Bull., 24(10), 1210-1213 (2001) https://doi.org/10.1248/bpb.24.1210
  13. Tsumura, Y., Yoshimaru, K., Tomaru, N., and Ohba, K., Molecular phylogeny of conifers using RFLP analysis of PCR-amplified specific chloroplast genes. Theoretical and Applied Genetics, 91, 1222-1236 (1995)
  14. Um, J. Y., Chung, H. S., Kim, M. S., Na, H. J., Kwon, H. J., Kim, J. J., Lee, K. M., Lee, S. J., Lim, J. P., Do, K. R., Hwang, W. J., Lyu, Y. S., An, N. H., and Kim, H. M. Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP. Biol. Pharm. Bull., 24(8), 872-875 (2001) https://doi.org/10.1248/bpb.24.872
  15. Wang, J., Ha, W. Y., Ngan, F. N., But, P. P., and Shaw, P. C., Application of Sequence Characterized Amplified Region (SCAR) Analysis to authenticate Panax species and their adulterants. Planta Med., 67(8), 781-783 (2001) https://doi.org/10.1055/s-2001-18340
  16. Wang, X., Sakuma, T., Asafu-Adjaye, E., and Shiu, G. K., Determination of Ginsenosides in Plant extracts from Panax ginseng and Panax quinquefolius L. by LC/MS/MS. Anal. Chem., 71(8), 1579-1584 (1999) https://doi.org/10.1021/ac980890p
  17. Yun, T. K., Lee, Y. S., Lee, Y. H., Kim, S. I., and Yun, H. Y., Anticarcinogenic effect of Panax ginseng C.A. Meyer and identification of active compounds. J. Korean Med. Sci., Suppl, S6-18 (2001)
  18. Yun, Y. P., Do, J. H., Ko, S. R., Ryu, S. Y., Kim, J. H., Song, H. C., Park, Y. D., Ahn, K. S., and Kim, S. H., Effects of Korean red ginseng and its mixed prescription on the high molecular weight dextran-induced blood stasis in rats and human platelet aggregation. Ethnopharmacol., 277(2-3), 259-264 (2001)