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The Pharmaceutical Society of Korea (대한약학회)
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Cystocin, a Novel Antibiotic, Produced by Streptomyces sp. GCA0001: Biological Activities

  • Published : 2003.06.01
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Abstract

Cystocin belongs to the class of nucleoside antibiotics from Streptomyces sp. GCA0001. Cystocin showed good activity against Gram-positive bacteria, but showed less activity against the Gram-negative bacteria. Cystocin exhibited about two to four folds higher activity than puromycin. Especially, cystocin shows relatively strong activity against Streptococcus strains. Cystocin shows quite potent antitumor activity against all of the cells tested showing $IC_{50}$ values of 0.10 to 0.14 $\mu$ g/mL. This in vitro result indicates that the cytotoxocity of cystocin is two ten folds more active than puromycin s.

Keywords

References

  1. Fong, K. L. and Vince, R., Peptidyl transferase substrate specificity with nonaromatic aminoacyl analogues of puromycin. J. Med. Chem., 21, 792-795 (1978) https://doi.org/10.1021/jm00206a014
  2. Harris, R. J., Hanlon, J. E., and Symons, R. H., Peptide bond formation on the ribosome. Structural requirements for inhibition of protein synthesis and of release of peptides from peptidyl-tRNA on bacterial and mammalian ribosome by aminoacyl and nucleotidyl analogues of puromycin. Biochim. Biophys. Acta, 240, 244-262 (1971) https://doi.org/10.1016/0005-2787(71)90664-2
  3. Herris, R. J. and Pestka, S., Molecular Mechanism of Protein Biosynthesis. Editors, Weissbach, H. and Pestka, S., Academic Press, 413-442 (1978)
  4. Kharel, M. K., Lee, H. C., Sohng J.-K., and Liou, K., Statistical Optimization of Medium Components for the Improved Production of Cystocin by Streptomyces sp. GCA0001. J. Ind. Eng. Chem., 8(5), 427-431 (2002) https://doi.org/10.1021/i500005a014
  5. Kieser, T., Bibb, M. J., Buttner, M. J., and Chater K. F., Media, buffers and suppliers. In Practical Streptomyces genetics. The John Innes Foundation, Norwich, 408p (2000)
  6. Poster, J. N., Hewitt, R. I., Hesseltine, C. W., Krupka, G., Lowery, J. A., Wallace, W. S., Bohonos, N., and Williams, J. H., J. Homonuclear Structure Dynamics, 2, 175-189 (1984) https://doi.org/10.1080/07391102.1984.10507556
  7. Sohng, J.-K., Lee, H. C., Liou, K., Lee E. B., Kang, S.-Y., and Woo, J.-S., Cystocin, A novel antibiotic, produced by Streptomyces sp. GCA0001: Production and Characterization of Cystocin. J. Microbiol. Biotecnol., in press (2003)
  8. Suhadolink, R. J., Nucleoside Antibiotics, Wiley Interscience, New York, 1-50, (1970)
  9. Suhadolink, R. J., Nucleosides as a Biological Probes, Wiley Interscience, New York, 96-102, (1979)