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The Pharmaceutical Society of Korea (대한약학회)
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The Polysaccharide Fraction AIP1 from Artemisia iwayomogi Suppresses Apoptotic Death of the Mouse Spleen Cells in Culture

  • Hwang, Jung-Suk (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Chung, Hye-Kyung (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Bae, Eun-Kyong (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Lee, Ah-Young (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Ji, Hee-Jung (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Park, Dong-Woon (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Jung, Hwa-Jin (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Cho, Chung-Won (Department of Microbiology and School of Biotechnology ＆ Biomedical Science, Inje University) ;
  • Choi, Hyun-Ju (Department of Medical Technology, Inje University) ;
  • Lee, Dong-Seok (Deaprtment of Medical Technology, Inje University) ;
  • Lee, Kang-Ro (Deaprtment of Medical Technology, Inje University) ;
  • Youn, Hyun-Joo (Deaprtment of Medical Technology, Inje University)
  • Published : 2003.04.01
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Abstract

A polysaccharide fraction, AIP1, purified from Artemisia iwayomogi was shown to have immunomodulating and anti-tumor activities in mice. In order to determine how the AIP1 fraction exhibits the immunomodulating activity, the effect of the fraction on the apoptosis of mouse spleen cells was investigated. Treatment of the mouse spleen cells with the AIP1 fraction resulted ,in the suppression of apoptotic death and an extension of cell survival in culture, indicating that the fraction might modulate the death of spleen cells. Treatment of the mice with the AIP1 fraction in vivo also resulted in less apoptosis of the spleen cells, which indicates the physiological relevance of the anti-apoptosis effect of the fraction in vitro. A mouse gene array was used to determine the profile of the gene expression change showing a pattern of up- and down-regulated genes by the AIP1 treatment. This study provides preliminary information regarding the immunomodulatory mechanism of the AIP1 fraction.

Keywords

References

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