대한의생명과학회지 (Biomedical Science Letters)
- 제9권4호
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- Pages.195-201
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- 2003
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Viability and Luciferase Activity of Freeze-Dried Recombinant Biosensor Cells for Detecting Aromatic Hydrocarbons
- Kim, Mi-Na (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
- Park, Hoo-Hwi (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
- Lim, Woon-Ki (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
- Shin, Hae-Ja (Environmental Engineering Major, Division of Applied Bioengineering, Dongseo University)
- 발행 : 2003.12.01
초록
Aromatic hydrocarbons are of major concern among genotoxic chemicals due to their toxicity and persistence. Some microorganisms can utilize aromatic hydrocarbons as carbon and energy sources by inducing expression of catabolic operon(s). The XylR regulatory protein activates transcription of the catabolic enzymes to degrade BTEX (benzene, toluene, ethylbenzene, and xylene) from its cognate promoters, Pu and Ps upon exposure of the cells to the aromatic hydrocarbons. The activity of XylR on the promoters was previously monitored using luciferase luc reporter system. The xylR, its promoter Pr and the promoter Po for the phenolic compound catabolic operon were introduced upstream of firefly luciferase luc in the pGL3b vector to generate about 7.1 kb of pXRBTEX. Here E. coli harboring the plasmid was freeze-dried under various conditions to fin,d optimal conditions for storage and transport. The cell viability and luciferase activity were maintained better, when the cells were freeze-dried at -7