한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제16권4호
  • /
  • Pages.289-295
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  • 2003
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지

미생물을 이용한 해조류의 가수분해 및 이용 III. 부식소나무의 미생물군으로부터 해조 분해능을 갖는 균주의 분리

Studies on the Hydrolysis of Seaweed using Microorganisms and Its Application III. Isolation of Seaweed Hydrolytic Strain from Microfloras in Decayed Pine Tree

  • 김해섭 (여수대학교 식품공ㆍ영양학부) ;
  • 최옥수 (순천제일대학 식생활부) ;
  • 강동수 (여수대학교 식품공ㆍ영양학부) ;
  • 김지만 (여수대학교 식품공ㆍ영양학부) ;
  • 김귀식 (여수대학교 식품공ㆍ영양학부) ;
  • 배태진 (여수대학교 식품공ㆍ영양학부)
  • 발행 : 2003.12.01
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⟨ 이전 논문 다음 논문 ⟩

초록

해조 가수분해능이 우수할 것으로 추정되는 부식한 소나무에서 채취한 미생물군 시료에 대하여 다시마분말 첨가 고체배지를 이용하여 4차에 걸쳐 순수배양을 실시하였다. 그 결과 16균주를 분리하였으며, 이들 균주를 다시마분말 첨가 액체배지에서 3주간 배양하며, 1주 간격으로 전당과 환원당의 함량을 측정한 결과 배양 전 기간에 08A211, 08C221 및 08B121균주가 모든 균주 중에 대체로 높은 분해율을 보였다. 이렇게 하여 선택된 3균주, 즉 08A211, 08B121 및 08C221균주에 대하여 sodium alginate, 다시마 및 미역분말이 첨가된 액체배지에서 균체성장과 분해율은 측정한 결과 08B121균주가 가장 높은 균체 성장과 다시마 및 미역 분해율을 나타내었다. 따라서 본 균주에 해조의 다당 또는 다른 성분을 가수분해시키는 효소가 존재할 것으로 추측된다.

This is a part of study on the hydrolysis of seaweed using microorganisms. A microflora sample obtained from a decayed pine tree was purified by pure culture of 4 times. As the result, 16 isolated strains were obtained from the microflora sample and then each strain was incubated in a liquid medium with sea tangle powder for 3 weeks. Ratios of reduced sugar to total sugar of 08A211, 08C221 and 08B121 strains were highest. Accordingly, these three strains were incubated in 3 different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week. Cell growth and ratios of reduced sugar to total sugar was highest for 08B121 in all the liquid media.

키워드

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