The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Studies on the Hydrolysis of Seaweed using Microorganisms and Its Application III. Isolation of Seaweed Hydrolytic Strain from Microfloras in Decayed Pine Tree

미생물을 이용한 해조류의 가수분해 및 이용 III. 부식소나무의 미생물군으로부터 해조 분해능을 갖는 균주의 분리

  • 김해섭 (여수대학교 식품공ㆍ영양학부) ;
  • 최옥수 (순천제일대학 식생활부) ;
  • 강동수 (여수대학교 식품공ㆍ영양학부) ;
  • 김지만 (여수대학교 식품공ㆍ영양학부) ;
  • 김귀식 (여수대학교 식품공ㆍ영양학부) ;
  • 배태진 (여수대학교 식품공ㆍ영양학부)
  • Published : 2003.12.01
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Abstract

This is a part of study on the hydrolysis of seaweed using microorganisms. A microflora sample obtained from a decayed pine tree was purified by pure culture of 4 times. As the result, 16 isolated strains were obtained from the microflora sample and then each strain was incubated in a liquid medium with sea tangle powder for 3 weeks. Ratios of reduced sugar to total sugar of 08A211, 08C221 and 08B121 strains were highest. Accordingly, these three strains were incubated in 3 different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week. Cell growth and ratios of reduced sugar to total sugar was highest for 08B121 in all the liquid media.

해조 가수분해능이 우수할 것으로 추정되는 부식한 소나무에서 채취한 미생물군 시료에 대하여 다시마분말 첨가 고체배지를 이용하여 4차에 걸쳐 순수배양을 실시하였다. 그 결과 16균주를 분리하였으며, 이들 균주를 다시마분말 첨가 액체배지에서 3주간 배양하며, 1주 간격으로 전당과 환원당의 함량을 측정한 결과 배양 전 기간에 08A211, 08C221 및 08B121균주가 모든 균주 중에 대체로 높은 분해율을 보였다. 이렇게 하여 선택된 3균주, 즉 08A211, 08B121 및 08C221균주에 대하여 sodium alginate, 다시마 및 미역분말이 첨가된 액체배지에서 균체성장과 분해율은 측정한 결과 08B121균주가 가장 높은 균체 성장과 다시마 및 미역 분해율을 나타내었다. 따라서 본 균주에 해조의 다당 또는 다른 성분을 가수분해시키는 효소가 존재할 것으로 추측된다.

Keywords

References

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