Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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ANTIBACTERIAL EFFECT OF POLYPHOSPHATE ON ENDODONTOPATHIC BACTERIA

근관감염균에 대한 polyphosphate의 항균효과

  • Shin, Jeong-Hee (Department of Conservative Dentistry, Division of Dentistry, Graduate School of Kyung Hee University) ;
  • Park, Sang-Jin (Department of Conservative Dentistry, Division of Dentistry, Graduate School of Kyung Hee University) ;
  • Choi, Gi-Woon (Department of Conservative Dentistry, Division of Dentistry, Graduate School of Kyung Hee University)
  • 신정희 (경희대학교 대학원 치의학과 치과보존학교실) ;
  • 박상진 (경희대학교 대학원 치의학과 치과보존학교실) ;
  • 최기운 (경희대학교 대학원 치의학과 치과보존학교실)
  • Published : 2003.11.01
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Abstract

This study was performed to observe the antibacterial effect of polyphosphate (polyP) with various chain lengths (P3~P75) on virulent. invasive strains of P. gingivalis A7A1-28 and W50, and multidrug resistant E. faecalis ATCC29212. P. gingivalis strains were grown in brain-heart infusion broth (BHI) containing hemin and vitamin K with or without polyP. PolyP was added at the very beginning of the culture or during the exponential growth phase of the culture. Inhibition of the growth of P. gingivalis was determined by measuring the absorbancy at 540nm of the grown cells. Viable cell counts of the culture and release of intracellular nucleotide from P. gingivalis were measured. E. faecalis was grown in plain BHI with antibiotics alone or in combination with polyP(calgon: 0.1~1.0%) and the bacterial absorbancy was measured. The overall results suggest that polyP has a strong antibacterial effect on the growth of the virulent strains of P. gingivalis and the antibacterial activity of polyP seems largely bactericidal. accompanying bacteriolysis in which chelation phenomenon is not involved. Although polyP does not exert antibacterial activity against E. faecalis, it appears to increase antibacterial effect of erythromycin and tetracycline on the bacterium. Therefore, polyP alone or in combination with antibiotics may be developed as a candidate for the agent controlling oral infections including endodontic infection.

Keywords

References

  1. Dahlen G and Moller AJR: Microbiology of endodontic infections. p,444 475. In Slots, J. and Taubman M.A. (ed.), Contemporary oral microbiology and immunology, Mosby Year Book Inc. St. Louis, 1992
  2. Miller WD: Microorganisms of the human mouth. S.S. White Dental Co. Philadelphia, 1890
  3. Henrici AT and Hartzell T.B.: The bacteriology of vital pulp. Res J, 1:419 422, 1919 https://doi.org/10.1177/00220345190010040201
  4. Haapasalo M: Black pigmented Gram negative anaer obes in endodontic infections. FEMS Immunol Med Microbiol, 6:213 218, 1993 https://doi.org/10.1111/j.1574-695X.1993.tb00329.x
  5. Kobayashi T, Hayashi A Yoshikawa R, Okuda K and Hara K: The microbial flora from root canals and periodontal pockets of non vital teeth associated with advanced periodontitis. Int Endod J, 23:100 106, 1990 https://doi.org/10.1111/j.1365-2591.1990.tb00847.x
  6. Van Winkelhoff AJ, Carlee AW and de Graaff J:Bacteroides endodontalis and other black pigmented Bacteroides species in odontogenic abscesses. Infect Immun. 49: 494 497,1985
  7. Haapasalo M: Bacteroides spp. in dental root canal infections. Endod Dent Treometot. 5:110, 1989
  8. Sundqvist G, Johansson E and Sjogren U: Prevalence of black pigmented Bacteroides species in root canal infections. J Endod, 15:13 19, 1989 https://doi.org/10.1016/S0099-2399(89)80092-5
  9. Rupf S, Eannengieeser S, Merte K, Pfister W, Sigusch B and Eschrich K: Comparison of profiles of key periodontal pathogens in periodontium and endodontium. Endod Dent Traumatol. 16:269 275, 2000 https://doi.org/10.1034/j.1600-9657.2000.016006269.x
  10. Rccae IN, Siqueira JFJr, Santos KR and Coelho AM: 'Red complex' (Bacteroides forsythus, Porphyromones gingivalis, and Treponema denticola) in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91:468-471, 2001 https://doi.org/10.1067/moe.2001.114379
  11. Sundqvist G: Bacteriological studies of necrotic dental pulps. Thesis. Umea University, Odontology, Diss ertation No.7, 1976 (cited from the reference No.4)
  12. Griffee MB, Patterson SS, Miller CH, Kafrawy AH and Newton CW: The relationship of Bacteroides melanino genicus to symptoms associated with pulpal necrosis. Oral Surg Oral Med Oral Pethol. 50:457 461. 1980 https://doi.org/10.1016/S0030-4220(80)80015-6
  13. Yoshida M, Fukushima H, Yamamoto K, Ogawa K, Toda T and Sagawa H: Correlation between clinical symptoms and microorganisms isolated from root canals with periapical lesions. J Endod, 13:24 28, 1987 https://doi.org/10.1016/S0099-2399(87)80088-2
  14. Haapasalo M, Ranta H, Ranta K and Shah H: Black pigmented Bacteroides spp. in human apical periodontitis. Infect Immun. 53:149 153,1986
  15. Mayrand D and Holt SC: Biology of assccharolytic black pigmented Bacteroides species. Microbiol Rev, 52: 134 152, 1988
  16. Socransky SS and Haffajee AD: Microbial mechanisms in the pathogenesis of destructive periodontal diseases: a critical assessment. J Periodont Res, 26:195 212,1991 https://doi.org/10.1111/j.1600-0765.1991.tb01646.x
  17. Van Winkelhoff AJ, van Steenbergen TJM, Kippuw N and de Graaff J: Enzymatic characterization of oral and nonoral black pigmented Bacteroides species. Anton. Van Leeuwenh., 52:163 171, 1986 https://doi.org/10.1007/BF00429320
  18. Nakamura R, Hinode D, Terai H and Morioka M: Extracellualr enzymes of Porphyromonas (Bacteroides) gingivalis in relation to periodontal destruction. P.129 141, In S. Hamada, S.C. Holt, and J.R. McGhee (ed.), Periodontal diseases: pathogens and host immune responses, Quintessence Publishing Co.. Ltd.. Tokyo, 1991
  19. Wingrove JA, DiScipio RG, Chen Z, Potempa J, Travis J and Hugli TE: Activation of complements C3 and C5 by a cysteine proteinase (Gingipain 1) from Porph yromonas (Bacteroides) gingivalis, J Biol Chem, 267:18902 18907, 1992
  20. Frandsen EVG: Hydrolysis of immunoglobulins. p.219 226. In H,N. Shah, D. Mayrand. and R.J. Genco (ed.), Biology of the species Porphyromonas gingivalis, CRC Press, Boca Raton, 1993
  21. Jagels MA, Travis J, Potempa J, Pike R and Hugli TE: Proteolytic inactivation of the leukocyte C5a receptor by proteinases derived from Porphyromonas gingivalis, Infect Immun. 64:1984 1991. 1996
  22. Tai H, Kobayashi T and Hara K: Changes in complement and immunoglobulin G receptor expreassion on neutrophile associated with Porphyromonas gingivalis induced inhibition of phagocytosis. Infect Immun. 61:3533 3535, 1993
  23. Ding Y, Haapasalo M, Kerosuo E, Lounatmaa K, Kotiranta A and Sorsa T: Release and activation of human neutrophil matrix metallo and serine protemases during phagocytosis of Fusobacterium nucleatum, Porphyromonas gingivalis and Treponema denticola, J Clin Periodontol, 24:237 248, 1997 https://doi.org/10.1111/j.1600-051X.1997.tb01837.x
  24. Hamada, N, Watanabe K, Arai M, Hiramine H and Umemotc T: Cytokine production induced by a 67 kDa fimbrial protein from Porphyromonas gingivalis, Oral Microbiol Immunol. 17:197 200, 2002 https://doi.org/10.1034/j.1399-302X.2002.170311.x
  25. Lee JY, Sojar HT, Bedi GS and Genco RJ: Porphyromonas (Bacteroides) gingivalis fimbrillin:size, amino terminal sequence, and antigenic heterogeneity. Infect Immun. 59:383 9, 1991
  26. Amano A, Nakagawa I. Kataoka K, Morisaki I and Hamada S: Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients. J Clin Microbiol. 37:1426 1430,1999
  27. Genco CA, Cutler CW, Kapczynski D, Maloney K and Arnold RR: A novel mouse model to study the virulence of and host response to Porphyromonas (Bacteroides) gtrigivalis, Infect Immun. 59:1255 1263, 1991
  28. Evans RT, Klausen B, Ramamurthy NS, Golub LM, Sfintescu C and Genco RJ.: Periodontopathic potential of two strains of Porphyromonas gingivalis in gnotobiotic rat. Arch Oral Biol, 37:813 819, 1992 https://doi.org/10.1016/0003-9969(92)90115-O
  29. Katz J, Ward DC and Michalek SM.: Effect of host responses on the pathogenicity of strains of Porphyromonas gingivalis, Oral Microbiol Immunol, 11:309 318, 1996 https://doi.org/10.1111/j.1399-302X.1996.tb00187.x
  30. Ebersole JL, Kesavalu L, Schneider SL, Machen RL and Holt SC: Comparative virulence of periodon topathogena in a mouse abscess model. Oral Dis, 1:115 128,1995 https://doi.org/10.1111/j.1601-0825.1995.tb00174.x
  31. Baker PJ, Dixon M, Evans RT and Roopenian DC: Heterogeneity of Porphyromonas gingivalis strains in the induction of alveolar bone loss in mice. Oral Microbiol. Immunol, 15:27 32, 2000 https://doi.org/10.1034/j.1399-302x.2000.150105.x
  32. Sundqvist G. Figdor D. Persson S and Sjogren U: Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85:86 93,1998 https://doi.org/10.1016/S1079-2104(98)90404-8
  33. Hancock HH. 3rd., Sigurdsson A, Trope M and Moiseiwitsch J: Bacteria isolated after unsuccessful endodontic treatment in a North American population, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91:579 586, 2001 https://doi.org/10.1067/moe.2001.113587
  34. Morrison D, Woodford N and Cookson B : Enterococci as emerging pathogens of humans. J Appl Microbiol, 83:S89 S99, 1997 https://doi.org/10.1046/j.1365-2672.83.s1.10.x
  35. Jett BD, Huycke MM and Gilmore MS: Virulence of enterococci. Clin Microbiol Rev, 7:462 478, 1994 https://doi.org/10.1128/CMR.7.4.462
  36. Whlin YB and Holm AK: Changes in the oral microflora in patients with acute leukemia and related disorders during the period of induction therapy. Oral Surg Oral Med Oral Pathol, 65:411 417, 1988 https://doi.org/10.1016/0030-4220(88)90354-4
  37. Rams TE, Feik D, Lisgarten MA and Slots J: Enterococci in human periodontitis. Oral Microbiol Immunol. 7:249 252, 1992 https://doi.org/10.1111/j.1399-302X.1992.tb00034.x
  38. Dahlen G, Semuelsson W, Molander A and Reit C: Identification and antimicrobial susceptibility of enterccccci isolated from the root canal. Oral Microbiol Immunol, 15:309 312, 2000 https://doi.org/10.1034/j.1399-302x.2000.150507.x
  39. Linden PK: Treatment options for vancomycin resistant enterococcal infections. Drugs, 62:425 441, 2002
  40. Lima KC, Fava LR and Siqueira JF Jr.: Susceptibilities of Enterococcus faecalis biofilms to some antimicrobial medications. J Endod, 27:616 619, 2001 https://doi.org/10.1097/00004770-200110000-00004
  41. Gomes BP, Ferraz CC, Vienna ME, Berber VB, Teixeira FB and Souza Filho FJ: In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J, 34:424 428, 2001 https://doi.org/10.1046/j.1365-2591.2001.00410.x
  42. Almyroudi A, Mackenzie D, McHugh S and Saunders WP: The effectiveness of various disinfectants used as endodontic intracanal medications: an in vitro study. J Endod, 28:163 167, 2002 https://doi.org/10.1097/00004770-200203000-00005
  43. Love RM: Entercococus faecalis a mechanism for itsrole in endodontic failure. Int Endod J, 34:399 405, 2001 https://doi.org/10.1046/j.1365-2591.2001.00437.x
  44. 0rstavik D and Haapasalo M: Disinfection by endodcntic irriganta and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol. 6:142 149, 1990 https://doi.org/10.1111/j.1600-9657.1990.tb00409.x
  45. Haapasalo HK, Siren EK, walttmc TM, Orstavfk D and Haapasalo MP.: Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J, 33:126 31, 2000 https://doi.org/10.1046/j.1365-2591.2000.00291.x
  46. Molander A, Reit C and Dahlen G: The antimicrobial effect of calcium hydroxide in root canals pretreated with 5% iodine potassium iodide. Endod Dent Traumatol. 15:205 9, 1999 https://doi.org/10.1111/j.1600-9657.1999.tb00775.x
  47. Siren EK, Haapasalo MP, Ranta K Salmi P and Kerosuo EN: Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. Int Endod J, 30:91 95, 1997 https://doi.org/10.1111/j.1365-2591.1997.tb00680.x
  48. Fuss Z, Charniaque O, Pilo R and Weiss E: Effect of various mixing ratios on antibacterial properties and hardness of endodontic sealers. J Endod, 26:519 522, 2000 https://doi.org/10.1097/00004770-200009000-00008
  49. Fuss Z, Weiss EI and Shalhav M: Antibacterial activity of calcium hydroxide containing endodontic sealers on Enterococcus faecalis in vitro. Int Endod J, 30:397 402, 1997
  50. Shalhav M, Fuss Z and Weiss EI: In vitro antibacterial activity of a glass ionomer endodontic sealer. J Endod, 23:616 619,1997 https://doi.org/10.1016/S0099-2399(97)80172-0
  51. 박석범, 최기운, 최호영:Plyphosphate가 함유된 근관충전재가 구강세균의 성장에 미치느 영향, 대한치과보존학회지. 26: 141 152, 2001
  52. Economides N, Kotsaki Kovatsi VP, Poulopouloa A, Kolokuris I. Rozos G and Shore R: Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues. J Endod, 21:122 127,1995 https://doi.org/10.1016/S0099-2399(06)80436-X
  53. Andres MT, Chung WO, Roberts MC and Fierro JF: Antimicrobial susceptibilities of Porphyromonas gingivalls, Prevotella intermedia. and Prevotella nigrescensspp. isolated in Spain. Antimicrob Agents Chemother, 42:3022 3023, 1998
  54. Chung WO, Gabany J, Persson GR and Roberts MC: Distribution of erm(F) and tet(Q) genes in 4 oral bacterial species and genotypic variation between resistant and susceptible isolates. J Clin Periodontol, 29:152 158, 2002 https://doi.org/10.1034/j.1600-051x.2002.290210.x
  55. Kulaev IS: Biochemistry of inorganic polyphosphates, Rev Physiol Biochem Pharmacol. 73:131 158, 1975 https://doi.org/10.1007/BFb0034661
  56. Kornberg A, Narayama NR and Ault Riche D: Inorganic polvphosphate: a molecule of many functicns. Annu Rev Biochem, 68:89 125, 1999 https://doi.org/10.1146/annurev.biochem.68.1.89
  57. U. S. Department of Agriculture: Meat and poultry products: phosphates and sodium hydroxide. Federal Register Rule and Regulatlon 47:10779,1982
  58. Zaika LL and Kim AH: Effect of sodium polyphosphate on growth of Listeria monocytogenes, J Food Prot, 56:577 580, 1993 https://doi.org/10.4315/0362-028X-56.7.577
  59. Lee RM, Hartman PA, Stahr HM, Olson DG and Williams FD: Antibacterial mechanism of long chain polyphosphates in Staphylococcus aureus. J Food Prot, 57:289 294, 1994 https://doi.org/10.4315/0362-028X-57.4.289
  60. Tanzer JM and Hageage GJJr.: Polyphosphate inhibition of growth of plaque formed by streptococci and diphtheroids implicated in oral disease. Infect Immun. 1:604 606,1970
  61. Shibata H and Morioka T: Antibacterial action of condensed phosphates on the bacterium Streptococcus mutans and experimental caries in the hamster. Arch Oral Blol, 27:809 816, 1982 https://doi.org/10.1016/0003-9969(82)90034-6
  62. 공희정, 최호영, 민병순, 박상진, 이진용, 최기운: 구강세균 Prevotella lntermedia의 성장에 따른 polyphosphate 의 영향에 관한 연구. 대한치과보존학회지, 23:550 560, 1998
  63. 최인식, 박병래, 김홍렬, 신세원, 최유진, 최영철. 이진용:Porphyromonas gingivalis에 대한 pclyphosphate의 향균 효과. 대한미생물학회지, 34:285 301, 1999
  64. Ayres HM, Furr JR and Russell AD: Effect of permeahilizers on antibiotic sensitivity of Pseudomonas aeruginosa: Lett Appl Microbiol, 28:13 16, 1999 https://doi.org/10.1046/j.1365-2672.1999.00486.x
  65. Socransky SS, Haffajee AD, Cugini MA, Smith C and Kent RLJr.: Microbial complexes in subgingival plaque. J Clin Periodontol, 25: 134 144, 1998 https://doi.org/10.1111/j.1600-051X.1998.tb02419.x
  66. Yao ES, Lamont RJ, Leu SP and Weinberg A: Interbacterial binding among strains of pathogenic and commensal oral bacterial species. Oral Microbiol Immunol. 11:35 41. 1996 https://doi.org/10.1111/j.1399-302X.1996.tb00334.x
  67. Niliue AM, Spencer SC and Simonson LG: Stimulation of in vitro growth of Treponema dentlcola by extraoel lular growth factors produced by Porphyromonas gingivalis, J Dent Res, 72:1027 1031. 1993 https://doi.org/10.1177/00220345930720060601
  68. Torabinejad M, Hong CU, Pitt Ford TR and Kettering JD: Antibacterial effets of some root end filling materials. J Endod, 21: 403 406, 1995 https://doi.org/10.1016/S0099-2399(06)80824-1
  69. Jones ME, Gesu G, Ortisi G, Sahm DF, Critchley IA and Goglio A: Proficiency of Italian clinical laboratories in detecting reduced glycopeptide susceptibility in Enterococcus and Staphylococcus spp. using routine laboratory methodologies. Clin Microbiol Infect, 8:101 111, 2002 https://doi.org/10.1046/j.1469-0691.2002.00378.x
  70. Chow JW, Davidson A Sanford E 3rd and Zervos MJ: Superinfection with Enterococcus faecalis during quinupriatin/dalfopriatin therapy. Clin Infect Dis, 24:91 92,1997 https://doi.org/10.1093/clinids/24.1.91
  71. 최성백, 박상진, 최기운, 최호영: Porphyromonas endodontalis에 대한 pclyphoephate의 항균기전에 관한 연구. 대한치과보존학회지. 25:561 574, 2000
  72. 강계숙: Mutans streptococci에 대한 polyphcsphate의 항균 효과. 경희대학교 박사논문.2001
  73. 김태진:Candida alhicans에 대한 polyphosphate 의 항균효과.경희대학교 석사논문. 2002
  74. Knabel SJ, Walker HW and Hartman PA: Inhibition of Aspergillus flavus and selected Gram positive bacteria by chelation of essential metal contains by polyphos phate. J Food Prot, 54:360 365, 1991 https://doi.org/10.4315/0362-028X-54.5.360
  75. Ellwood DC and Tempest DW: Effects of environment on bacterial wall content and composition. Adv Microb Physiol, 7:83 117, 1972 https://doi.org/10.1016/S0065-2911(08)60077-6
  76. Post FJ and Krishnamurty GB, Flanagan MD: Influence of sodium hexametaphosphate on selected bacteria. Appl Microblol, 11:430 435,1963
  77. Van Steenbergen TJM, Kastelein P,Touw JJA and de Graaff J: Virulence of black pigmented Bacteroides strains from periodontal pockets and other sites in experimentally induced skin lesions in mice. J Periodontal Res, 17:41 49, 1982 https://doi.org/10.1111/j.1600-0765.1982.tb01129.x
  78. Neiders ME, Chen PB, Suido H, Reynolds HS, Zambon JJ, Shlossman M and Genco RJ: Heterogeneity of virulence among strains of Bacteroides gingivalis . J Periodontal Res, 24:192 198, 1989 https://doi.org/10.1111/j.1600-0765.1989.tb02005.x
  79. Chen PB, Neiders ME, Millar SJ, Reynolds HS and Zambon JJ: Effect of immunization on experimental Bacteroides gingivalis infection in a murine model. Infect Immun. 55:2534 2537, 1987
  80. Engstrom B: The significance of enterococci in root canal treatment. Odont Revy, 15:87 106, 1964
  81. Ayres H, Furr JR and Russell AD: A rapid method of evaluating permeabilizing activity against Pseudomonas aeruginosa, Lett Appl Microbiol, 17: 149 151. 1993 https://doi.org/10.1111/j.1472-765X.1993.tb00381.x
  82. Seymour RA and Heasman FA: Tetracyclines in the management of periodontal diseases. A review. J Clin Periodontol, 22:22 35, 1995 https://doi.org/10.1111/j.1600-051X.1995.tb01767.x
  83. Vemillo AT and Rifkin BR: Effects of tetracyclines on bone metabolism. Adv Dent Res, 12:56 62, 1998 https://doi.org/10.1177/08959374980120012101
  84. Lee JY, Ryu DM, Shin and JW: Inorganic polyphoaphate promotes bone regeneration. Korean J Oral Anat, 23:219 235, 1999

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