Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effect of Proliferation Inhibition on B16/F10 Melanoma Cell by Chloroform Extract from Cornis fructus

산수유 클로로포름 추출물에 의한 B16/F10 melanoma세포의 증식억제효과

  • 최원형 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 천현자 (원광대학교 자연과학기술부) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2003.06.01
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Abstract

Cornis fructus have various biological effects and major chemical components have been tannins, saponins, ursolic acids, gallic acids, linoleic acids, morronisides, cornins and loganins. The main aim of the present study is measurment the effect of chloroform extract from Cornis fructus on proliferation inhibition and Cell death. Cells were cultured in the presence of chloroform extracts from Cornis fructus for 48 h. after 48h treatment of B16/F10 melanoma cells with chloroform extracts, the cells were observed a dose-dependent inhibitions of cell viability with cell death in their proliferation. the cells were estimated cell viability, cell number, total DNA fragmentation and chromatin condensation in a dose-dependent manner. It also caused cell death as measured by cell morphology, DNA fragmentation and nucleus chromatin condensation. therefore, these results suggest that chloroform extracts from C. fructus is inhibitory proliferation and is related to cell death in this cells.

Keywords

References

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