DOI QR코드

DOI QR Code

In vitro 검출시스템을 이용한 해양생물 추출물로부터 에스트로겐 활성 검증

Verification of Estrogenic Activity in Ethanol Extracts of Marine Organisms Using in vitro Test System.

  • 하종명 (신라대학교 생명공학과, 마린 바이오 산업화 지원센터) ;
  • 이상현 (신라대학교 생명공학과, 마린 바이오 산업화 지원센터)
  • 발행 : 2003.12.01

초록

천연물에 포함되어 있는 에스트로겐 효과를 가지는 성분의 생체 내에서의 직접적인 효과를 검정하기 위하여, 에스트로겐 수용체를 발현하는 것으로 알려진 인체 유방암 세포주 MCF7에 에스트로겐에 반응성을 나타내도록 고안된 CAT 리포터 플라스미드를 도입한 in vitro 검출시스템을 사용하여 에스트로겐 활성을 측정하였다. 이미 여러 분야에서 그 기능성의 연구가 활발히 진행되고 있는 광합성 조류인 스피루리나(spirulina)와 파래 등의 해양식물을 대상으로 에스트로겐 반응 리포터 시스템을 이용하여 에스트로겐 활성을 측정하였다. 그 결과, 스피루리나 에탄올 추출물의 CAT 활성은 $500\mug/ml\; 와\; 50 \mug/ml$의 농도에서 표준물질인 $17\beta-estradiol$의 농도 $10^{-8}$ M과 비슷한 정도의 에스트로겐 활성 효과를 나타내었고, $5\mug/ml$의 농도에서는 표준물질인 $17\beta-estradiol$의 농도 $10^{-10}$ M과 비슷한 정도의 에스트로겐 활성 효과를 나타내었다. 하지만 파래 추출물의 경우에는 에탄올을 처리한 대조군과 비교하여 유의한 CAT활성 변화를 나타내지 않았다 한편, 불가사리와 새우 등의 해양동물을 대상으로 한 실험에서는 에탄올을 처리한 대조군과 비교하여 유의한 CAT 활성 변화를 나타내지 않았다. 이 연구 결과로 광합성 조류인 스피루리나에 에스트로겐 활성을 효과적으로 나타내는 생리활성성분이 포함되어 있을 수 있다는 가능성을 확인하였다.

In order to verify the occurrence of an estrogenic compound in natural products, the estrogenic activity was measured using an in vitro detection system. For this system, human breast cancer cell line MCF7 was transfected using an estrogen responsive CAT reporter plasmid. Estrogenic activities of photosynthetic algae spirulina and sea lettuce were evaluated using this system. Estrogenic activities of a $500\mug/ml\; and\; 50 \mug/ml$ ethanol extracts of spirulina were as much as that of $10^{-8}$M standard solution (17$\beta$-estradiol) and activity of $5\mug/ml$ ethanol extract of spirulina was as much as that of $10^{-10}$ M standard solution. However, no significant estrogenic activity was observed using sea lettuce extract. Estrogenic activities of marine animals, such as star fish and shrimp, were also evaluated using this system, however, no significant estrogenic activity was observed in these extracts. In this result, it is confirmed that spirulina extract possesses estrogenic compound.

키워드

참고문헌

  1. Maturitas v.42 The nature and utility of the phytoestrogens: A review of the evidence Albertazzi,P.;D.W.Purdie https://doi.org/10.1016/S0378-5122(02)00024-5
  2. Baillierre's Clin. Endocrinol. Metab. v.12 Phytoestrogen in bone Anderson,J.B.;S.C.Garner https://doi.org/10.1016/S0950-351X(98)80003-7
  3. J. Nutr. v.126 Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys Anthony,M.S.;T.B.Clarkson;C.L.Hughes;T.M.Morgan;G.L.Burke
  4. Eur. J. Obstet. & Gynecol. v.85 Phytoestrogens: the natural selective estrogen receptor modulators Brzezinski,A.;A.Debi https://doi.org/10.1016/S0301-2115(98)00281-4
  5. J. Nat. Prod. v.56 Starfish saponins. Part 50. Steroidal glycosides from the Okinawan starfish Nardoa tuberculata Bruno,I.;L.Minale;R.Riccio;L.Cariello;T.Higa;J.Tanaka https://doi.org/10.1021/np50097a008
  6. J. Nat. Prod. v.61 Starfish saponins. 55. Super (1) Isolation, structure elucidation, and biological activity of the steroid oligoglycosides from an antarctic starfish of the family Asteriidae De Marino,S.;M.Iorizzi;E.Palagiano;F.Zollo;C.Roussakis https://doi.org/10.1021/np970500e
  7. Endocrinol. v.140 Differential interaction of the methoxychlor metabolite 2,2-bis-(p-hydroxyphenyl)-1,1,1-trichloroethane with estrogen receptors alpha and beta Gaido,K.W.;L.S.Leonard;S.C.Maness;J.M.Hall;D.P.McDonnell;B.Saville;S.Safe https://doi.org/10.1210/en.140.12.5746
  8. Environ. Health Perspect. v.107 Serum vitellogenin levels and reproductive impairment of male Japanese Medaka (Oryzias latipes) exposed to 4-tert-octylphenol Gronen,S.;N.Denslow;S.Manning;S.Barnes;D.Barnes;M.Brouwer https://doi.org/10.2307/3434542
  9. Tetrahed. Lett. v.43 Mycaloside A, a new steroid oligoglycoside with an unprecedented stucture from the Caribbean sponge Mycale laxissima Kalinovsky,A.I.;A.S.Antonov;S.S.Afiyatullov;P.S.Dmitrenok;E.V.Evtuschenko;V.A.Stonik https://doi.org/10.1016/S0040-4039(01)02184-0
  10. Ann. Rev. Nutr. v.17 Dietary phytoestrogens Kurzer,M.S.;X.Xu https://doi.org/10.1146/annurev.nutr.17.1.353
  11. J. Am. Col. Cardiol. v.35 Phytoestrogens and cardiovascular health Lissin,L.W.;J.P.Cooke https://doi.org/10.1016/S0735-1097(00)00590-8
  12. Braz. J. Med. Biol. Res. v.31 Antioxidant activity of the microalga Spirulina maxima Miranda,M.S.;R.G.Cintra;S.B.Barros;J.Mancici Filho
  13. EMBO J. v.4 Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter Miyamoto,N.G.;V.Moncollin;J.M.Egly;P.Chambon
  14. Int. J. Food Microbiol. v.45 Lactic acid bacteria growth promoters from Spirulina platensis Parada,J.L.;G.Z. de Caire;M.C.Z. de Mule;M.M.S. de Cano https://doi.org/10.1016/S0168-1605(98)00151-2
  15. EMBO J. v.9 Synergistic activation of transcription by the human estrogen receptor bound to tandem responsive elements Ponglikitmongkol,M.;J.H.White;P.Chambon
  16. Am. J. Med. v.103 Role of estrogen replacement therapy in memory enhancement and the prevention of neuronal loss associated with Alzheimer's disease Simpkins,J.W.;P.S.Green;K.E.Gridley;M.Singh;N.C. de Fiebre;G.Rajakumar
  17. J. Ethnopharmacol. v.82 Estrogenic effects of ethanol and ether extracts of propolis Song,Y.S.;C.Jin;K.J.Jung;E.H.Park https://doi.org/10.1016/S0378-8741(02)00159-9
  18. Toxicol. Lett. v.102 Dietary phytoestrogens and their role in hormonally dependent disease Strauss,L.;R.Santti;N.Saarinen;T.Streng;S.Joshi;S.Makela https://doi.org/10.1016/S0378-4274(98)00332-4
  19. Proc. Natl. Acad. Sci. U.S.A. v.95 Effects of soybean phytoestrogen intake on low density lipoprotein oxidation resistance Tikkanen,M.J.;K.Wahala;S.Ojala;V.Vihma;H.Adlercreutz https://doi.org/10.1073/pnas.95.6.3106
  20. Science v.273 Identification of an estrogen response element activated by metabolites of 17β-estradiol and raloxifene Yang,N.N.;M.Venugopalan;S.Hardikar;A.Glasebrook https://doi.org/10.1126/science.273.5279.1222

피인용 문헌

  1. Verification of the Fractions with Strong Estrogenic Activities from Brown Algae vol.20, pp.12, 2010, https://doi.org/10.5352/JLS.2010.20.12.1807