Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
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Plasma Protein Adsorption to Anion Substituted Poly(vinyl alcohol) Membranes

  • Ryu, Kyu-Eun (Department of Biomedical Sciences, College of Medicine, Catholic University) ;
  • Hyangshuk Rhim (Department of Biomedical Sciences, Department of Internal Medicine, College of Medicine, Catholic University) ;
  • Park, Chong-Won (Division of Hematology, Department of Internal Medicine, Department of Internal Medicine, College of Medicine, Catholic University) ;
  • Chun, Heung-Jae (Department of Biomedical Sciences, Department of Internal Medicine, College of Medicine, Catholic University) ;
  • Hong, Seung-Hwa (Blood Products Division, Biologics Evaluation Department, Korea Food and Drug Administration) ;
  • Kim, Jae-Jin (Biomaterials Research Center, Korea Institute of Science and Technology) ;
  • Lee, Young-Moo (School of Chemical Engineering, College of Engineering, Hanyang University)
  • Published : 2003.12.01
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Abstract

Anion-substituted poly(vinyl alcohol) (PVA) membranes, carboxymethylated PVA (C-PVA), and sulfonated PVA (S-PVA) were prepared and the effects of these substitutions on the plasma protein adsorption were studied by one- and two-dimensional gel electrophoresis and immunoblotting. When Cuprophane was used as a negative control, the amount of total proteins bound to samples decreased in the order Cuprophane > PVA > C-PVA > S-PVA, which we attribute to the effects of the surface characteristics of the samples, such as their surface tensions and electrostatic properties, on the adsorption of proteins to the surfaces of the materials. The results revealed that albumin was the most abundant protein in all the samples. The proportion of adsorbed fibrinogen to S-PVA exceeded those of PVA and C-PVA, whereas S-PVA exhibited the lowest IgG adsorption affinity among the samples we studied.

Keywords

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