Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Micorpropagation of Corylopsis coreana by Thidiazuron Treatment

Thidiazuron 처리에 의한 히어리나무의 기내번식

  • Kang, Hyo-Jin (Division of Biotechnology, Forestry Research Institute (KFRI)) ;
  • Moon, Heung-Kyu (Division of Biotechnology, Forestry Research Institute (KFRI)) ;
  • Yi, Jae-Seon (Division of Forest Resources, College of Forest Science, Kangwon National University)
  • 강효진 (임업연구원 생물공학과) ;
  • 문홍규 (임업연구원 생물공학과) ;
  • 이재선 (강원대학교 산림자원학부)
  • Published : 2003.09.01
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Abstract

This study was conducted to evaluate the effect of thidazuron(TDZ) on shoot proliferation and growth from axillary buds of 20-years-old Corylopsis coreana. Shoots proliferation was effectively achieved on WPM(Woody Plant Medium) supplemented with 0.03∼0.1mg/L TDZ. The highest shoot number(6.5$\pm$0.7) was obtained on 0.1mg/L TDZ treatment. On the TDZ medium shoots formed as clusters less than 1cm in height and therefore needed to subculture on GA$_{3}$ containing medium to induce elongation. In consecutive cultures, phenolic compounds were excreted at the proximal part of the explants and inhibited growth of the explants. Growth inhibition by the compounds was overcome using liquid and paper bridge culture system. About 60％ of the elongated shoots rooted on half- strength MS medium containing IBA. Generally, IBA was mire effective on in vitro rooting than NAA with optimal range of 0.5mg/L to 1.0mg/L. Rooted plantlets were transferred in an artificial soil(vermculite) and acclimatized in high humidity greenhouse condition. Survival rate differed greatly depending on rooting types of the explants. Two types of rooting were observed. The first type was direct rooting from the explants. The second type was callus formation followed by rooting from the callus. The explants showing the 1st type rooting survived can be multiplicated in vitro by TDZ treatment followed by elongation with GA$_{3}$ and rooting with IBA.

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