Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Plant Regeneration Derived from Leaf Disk Cultures in Purple Sweetpotato

자색고구마의 잎 조직배양을 통한 식물체 재생

  • Park, Hyae-Jeong (Department of Biology, College of Natural Science, Chosun University) ;
  • Ahn, Young-Sup (Mokpo Experimental Station, Rural Develpment Administration) ;
  • Jeong, Byeong-Choon (Mokpo Experimental Station, Rural Develpment Administration) ;
  • Park, Hyeon-Yong (Department of Biology, College of Natural Science, Chosun University)
  • 박혜정 (조선대학교 자연과학대학 생물학과) ;
  • 안영섭 (호남농업시험장 목포시험장) ;
  • 정병춘 (호남농업시험장 목포시험장) ;
  • 박현용 (조선대학교 자연과학대학 생물학과)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to establish a regeneration system from leaf explant of purple sweetpotato(Ipomoea batatas L.) The optimal concentrations of plant growth regulators for callus induction and shoot formation were determined. The optimal combination for callus formation was 1$\mu$M 2,4-D 5$\mu$M BM, and highest yield of embryogenic calli were observed on Murashige and Skoog basal medium containing 0.5$\mu$M 2,4-D under light condition after 4weeks of culture. Embryogenec callus was subcultured on medium supplemented with 5$\mu$M ABA for 4 days. Subsequently, regeneration of adventitious shoots occurred when these embryogenic calli were transferred onto medium with 3∼6$\mu$M gibberellic acid. Regenerated shoots were developed into normal plantlets.

Keywords

References

  1. Dennis L, Bidney, James F, Shepard (1980) Colony development from sweet potato petiole protoplasts and mesophyll cells. Plant Sci Lett 18: 335-342 https://doi.org/10.1016/0304-4211(80)90096-6
  2. Dodds JH, Merzdorf C, Zambrano V, Siguenas C, Jaynes J (1991) Potential use of Agrobacterium-mediated gene transfer to confer insect resistance in sweet potato. In: Jansson RK, Raman KV (eds). Sweetpotato Pest Management; a Global Perspective. Westiew, Boulder, Colo, pp 203-219
  3. Fuleki T, Francis FJ (1968) Quantitative methods for anthocyanins. 1. Extraction and determination of tatal anthocyanin in cranberries. J Food Sci 33: 72-77 https://doi.org/10.1111/j.1365-2621.1968.tb00887.x
  4. Kwon EJ, Kwon SY, Kim MZ, Lee JS, Ahn YS, Jeong BC, Kwak SS, Lee HS (2002) Plant regeneration of major cultivars of sweetpotato (lipomoea batatas) in Korea via somatic embryogenesis. Koran J Plant Biotech 29: 189-192 https://doi.org/10.5010/JPB.2002.29.3.189
  5. Liu JR, Cantliffe DJ (1984) Somatic embryogenesis and plant regeneration in tissue cultures of sweetpotato (lpomoea batatas Poir). Plant Cell Rep 3: 112-115 https://doi.org/10.1007/BF02441013
  6. Liu JR, Cantliffe DJ, Simonds SC, Ruan JF (1989) High frequency somatic embryogenesis from cultured shoot epical meristem domes of sweetpotato (lpomoea batatas). SABRAO J 21:93-101
  7. Min SR, Liu JR, Rho TH, Kim CH, Ju JI (1994) High frequency somatic embryogenesis and plant regeneration in tissue culture of Korean cultivar sweetpotato. Korean J Plant Tissue Cult 21:157-160
  8. Dicomo F, Misawa M (1995) Plant cell and tissue culture: alternatives formetabolite production. Biotech Advances 13: 425-453 https://doi.org/10.1016/0734-9750(95)02005-N
  9. Murashige T, SkoofF (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  10. Newell CA, Lowe JM, Merrywether A, Rooke LM, Hamiltone WDO(1995) Transformation of sweetpotato (lpmoea balalas (L.)Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin. Plant Sci 107: 215-227 https://doi.org/10.1016/0168-9452(95)04109-8
  11. Otani M, Shimada T (1996) Efficient embryogenic callus formation in sweetpotato (Ipmoea batatas (L) Lam.) using Agrobacterium tumefaciens. Plant Biotech 15: 11-16
  12. Otani M, Shimada T, Nizeki H (1987) Mesophyll protoplast culture of sweetpotato (Ipomoea batatas L) Plant Sci 53: 157-160 https://doi.org/10.1016/0168-9452(87)90125-7
  13. Yoshimoto M (2001) New trends of processing and use of sweet potato in Japan. Farming Jpn 35: 22-28
  14. Phuc JE, Lindberg B, Ogle, Thcmke S (2001) Determination of the nutritive value of tropical biomass products for monogastrics using rats. Part 2. Effects of drying temperature. Asian-Australian J Anim Sci 14: 994-1002 https://doi.org/10.5713/ajas.2001.994
  15. Prakash CS, Varadarajan U (1992) Genetic transformation of sweetpotato by particle bombardment. Plant Cell Rep 11: 53-57
  16. Zheng Q, Dessai AP, Prakash CS (1996) Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis. Plant Cell Rep 6: 381-385

Cited by

  1. Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato vol.32, pp.4, 2005, https://doi.org/10.5010/JPB.2005.32.4.307