Antimicrobial Activities of Berry Extract of Domestic Plants on 4 Kind of Pathogenic Microorganism

4종의 식품 부패 미생물에 대한 국내산 자생 식물 열매 추출물의 항균성 탐색

  • 권민경 (성신여자대학교 식품영양학과) ;
  • 이해은 (성신여자대학교 식품영양학과) ;
  • 박주연 (성신여자대학교 식품영양학과) ;
  • 한영숙 (성신여자대학교 식품영양학과)
  • Published : 2003.10.01

Abstract

To select new useful domestic plants with antimicrobial activities, thirty five samples of berry plant were distributed from Plant Diversity Research Center in Korea Research Institute of Bioscience and Biotechnology. Their extracts with methanol were tested against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes by paper disc method. The methanol extracts from Viburnum dilatatum, Viburnum erosum, Aralia continentalis, Camellia japonica, Acer trumcatam, Arasaema takesimense and Rhysocarpus intermedius were effective against S. aureus and the results were as follows: Viburnum erosum 6.5 mm, Viburnum dilatatum and Aralia continentalis 7.0 mm, Rhysocarpus intermedius 8.0 mm, Acer trumcatum 9.0 mm, Camellia japonica and Arisaema takesimense 9.5 mm. And only the methanol extract from Camellia japonica was effective against L. monocytogenes, 7.0 mm. Antimicrobial activities of E. coli and S. typhymurium were not detected. The minimum inhibitory concentration(MIC) of berry of Camellia japonica was examined as 1,250 $\mu\textrm{g}$/mL against S. aureus and as 1,250∼2,500 $\mu\textrm{g}$/mL against L. monocytogenes.

4종류의 식중독 세균 E. coli, S. typhimurium, S. aureus, L. monocytogenes 에 대하여 35 종의 국내산 자생 식물 열매 추출물에 의한 항균작용을 조사하였다. 1 mg/disc 농도의 추출물을 6.0 mm paper disc 에 흡수시켰으며 disc 주위의 clear zone의 직경 (mm)을 생육저해환으로서 비교하였다. S. aureus에서는 덜꿩나무가 6.5 mm, 가막살나무와 독활이 각각 7.0 mm, 신나무가 8.0 mm 동백나무 와 만주고로쇠가 각각 9.5 mm의 clear zone을 보이며 우수한 항균성을 가진 것으로 검색되었고 L. monocytogenes에서는 동백나무 열매 추출물만 7.0 mm의 clear zone이 나타났으며 E. coli 와 S. typhimurium에서는 모든 추출물이 항균성을 나타내지 않았다. 또한 S. aureus 와 L. monocytogenes에 항균성을 보인 동백나무 열매 MeOH 추출물의 최소 저해 농도를 측정한 결과 S. aureus에 대하여 1,250 $\mu\textrm{g}$/mL, L. monocytogenes에 대하여 1,250∼2,500 $\mu\textrm{g}$/mL로 나타났다. 이상의 본 연구 결과는 35종의 국내산 자생 식물 열매의 항균 효과를 검토하고 동백나무 열매의 최소 저해 농도를 측정하여 향후 천연 항균 소재 개발에 필요한 기초 자료를 제시하였다.

Keywords

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