Bovine dermatophytosis and susceptibility of the isolates to antifungal drugs

소 피부사상균증의 발생상황 및 분리균의 약제감수성

  • 오강희 (경상북도가축위생시험소) ;
  • 박노찬 (경상북도가축위생시험소) ;
  • 도재철 (경상북도가축위생시험소) ;
  • 임소정 (경북대학교 수의과대학) ;
  • 박진희 (경북대학교 수의과대학)
  • Published : 2003.03.01

Abstract

The present study was conducted to examine the outbreak state of bovine dermatophytosis in 14 farms(4 dairy farms, 10 Korean indigenous cattle farms) in Gyeongbuk province from November 2000 to November 2001. The causative agents of dermatophytosis was identified by mycological examination. Antifungal susceptibility test of 26 isolates was performed by agar dilution method, using 5 antifungal drugs. Prevalence of bovine dermatophytosis was found to be 13.5%(90/665) in dairy cattle farms and 14.5%(220/1,520) in Korean indigenous cattle farms. The most common age at which this disease occurred was 2-12 months. This disease usually occurred from winter to spring and the occurrence subsequently decreased in the summer. But 4 Korean indigenous cattle farms with poorly hygienic status were occurred all the year round. The causative agent was identified as Trichophyton verrucosum exclusively in these case. Antifungal susceptibility test of T verrucosum (26 strains) was performed by agar dilution method, using 5 antifungal drugs including tolnaftate, griseofulvin, ketoconazole, amphotericin B and terbinafine. All isolates were highly sensitive to 5 antifungal drugs (geometric mean MICs 0.004∼0.032 $\mu\textrm{g}$/$m\ell$). The isolates were the most sensitive to especially tolnaftate.

Keywords

References

  1. Carter GR, Cole JR. 1994. Diagnostic procedures in veterinary bacteriology and mycology. Academic Press, Inc. 5th ed., Toronto:381-404
  2. Quinn PJ, Carter ME, Markey BK, et aI. 1994. Clinical veterinary microbiology. Wolfe, Barcelona:381-390
  3. Jungerman PF, Schwartzman RW. 1972. Veterinary medical mycology. Lea & Febiger, Philadelphia:3-28
  4. 이헌준, 조성환, 최원필 등. 1991. 소 피부사상균의 역학적 및 진균학적 연구. 한국수의공중보건학회지 15(3):247-253
  5. Takatori K, Takahashi A, Kavai S, et al. 1993. Isolation of Trichophyton verrucosum from lesional and nonlesional skin in calves. J Vet Med Sci 55(2):343-344
  6. Wabacha JK, Gitau GK, Bebora LC, et al. 1998. Occurrence of dermatomycosis (ringworm) due to Trichophyton uerrucosum in dairy calves and its spread to animal attendants. J S Afr Vet Assoc 69(4):172-173
  7. Gordon PJ, Bond R. 1996. Efficacy of a live attenuated Trichophyton verrucosum vaccine for control of bovine dermatophytosis. Vet Rec 139:295-396
  8. Moretti A, Boncio L, Pasquali P, et al. 1998. Epidemiological aspects of dermatophyte infections in horses and cattle. Zentralbl Veterinarmed (B) 45(4):205-208
  9. 최원필, 여상건, 이현범. 1979. 한우에 집단 발생한 백선균증에 관한 연구. 대한수의학회지 19(2):149-152
  10. 김영포, 전인기, 김승훈. 1986. Trichophyton verrucosum에 의한 두부독창과 역학적 관찰. 대한피부학회지 24:687-691
  11. 김성화, 오수희, 최성관 등. 1997. Trichophyton verrucosum에 의한 수발 백선 3예. 대한의진균학회지 2(1):59-64
  12. 신동훈, 김경수, 김기홍. 1998. 대구지방 두부백선에 대한 임상 및 진균학적 관찰. 대한의진균학회지 3(2):132-138
  13. Korting HC, Rosenkranz S. 1989. In vitro susceptibility of dermatophytes from Munich to griseofulvin, miconazole and ketoconazole. Mycoses 22:136-139
  14. Granade TC, Artis WM. 1980. Antimycotic susceptibility testing of dermatophytes in microcultures with a standardized fragmented mycerial inoculum. Antimicrob Agent Chemother 17:725-729 https://doi.org/10.1128/AAC.17.4.725
  15. 전이식, 서순봉. 1979. 피부진균증의 각종 항진균제에 대한 감수성. 대한피부학회지 17 :221-227
  16. 최원필, 주진숙. 1998. 항진균제에 대한 피부사상균의 감수성. 한국수의공중보건학회지 22(3):201-206
  17. 한기옥, 최원필. 2001. 개 피부병 유래의 Microsporum canis의 항진균제 감수성. 대한수의학회지 41(2):173-176
  18. Pelayo US. 1979. In vitro effectiveness of salicylic, benzoic and boric acid and iodine thymol solutions on various strains of dermatophytes, Rev Cubana Med Trop 31(2):121-126
  19. Makenzie DWR. 1963. 'Hairbrush diagnosis' in the detection and eradication of nonfluorescent scalp ring worm. Brit Med J 2:363-365 https://doi.org/10.1136/bmj.2.5353.363
  20. Yoshida T, Jono K, Okonogi K. 1997. Modified agar dilution susceptibility testing method for determining in vitro activites of antifungal agents, including azole compounds. Antimierob Agent Chemother 41:1349-1351
  21. Wawrzkiewicz K, Ziolkowska G. 1985. Application of a two layer method of cultivation to determine the susceptibility of Trichophyton verrucosum by a standard plate diffusion test. Mykosen 28(11):574-578
  22. 김연진, 최진혁, 방장석 등. 2000. Terbinafine으로 치료한 Trichophyton verrucosum에 의한 노인 두부독창 1예. 대한의진균학회지 5(3):129-134
  23. Tanaka I. 1995. Dermatophytosis in cattle Epidemiology, treatment, and prevention. J Nippon Vet Animal Sci Univ 44:111-112