Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Simultaneous Quantitative Determination of Multiple Analytes with Fluorescence- Tagged Probes by Immunochromatogratphy

  • Jeong, Dong-Seok (Central Research Institute of Boditechnology, Bioventure Inovation Center) ;
  • Choi, Eui-Yul (Department of Genetic Engineering, College of Natural Sciences, Hallym University)
  • Published : 2003.03.01
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Abstract

Immunoassays have become indispensable tools and achieved great importance in scientific and medical research. However, typical immunoassays are time-consuming and use complex, multi-step procedures. In this study, we introduce a new immunoassay system for the quantification of several analytes at a time without any washing steps. It is comprised of a detector solution with fluorescence-labeled antibodies and a test strip with immobilized capture antibodies. Using a micro-array scanner, the antigen-antibody complex was quantitatively determined by measuring the intensities of fluorescence on the capture lines or dots of nitrocellulose membrane. This method demonstrated its rapid quantitative determination of analytes without many processing steps as well as specific identification of multiple analytes in biological specimens.

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