Journal of Microbiology and Biotechnology

  • 제13권4호
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  • Pages.487-494
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  • 2003
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Purification and Characterization of 2,3-Dihydroxybiphenyl 1,2-Dioxygenase from Comamonas sp. SMN4

  • Lee, Na-Ri (Department of Biological Science, Sookmyung Women's University, Korea Food Research Institute) ;
  • Lee, Jang-Mi (Department of Biological Science, Sookmyung Women's University) ;
  • Min, Kyung-Hee (Department of Biological Science, Sookmyung Women′s University) ;
  • Kwon, Dae-Young (Korea Food Research Institute)
  • 발행 : 2003.08.01
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초록

2,3-Dihydroxybiphenyl 1,2-dioxygenase (23DBDO), an enzyme of the biphenyl biodegradation pathway encoded by the bphC gene of Comnmonas sp. SMN4, was expressed and purified using column chromatographies. SDS-PAGE of purified 23DBDO showed a single band with a molecular mass of 32 kDa, which was consistent with the data from the gel filtration chromatography (GFC). The purified enzyme exhibited a maximum 23DBDO activity at pH 9.0 and was stable at pH 8.0. The enzyme showed maximum activity at $40^{\circ}C$ and maintained activity at $30^{\circ}C$ for 24 h. Kinetic parameters represented by Michaelis-Menten constants such as $K_m\;and\;V_{max}$ values for various substrates were determined by Lineweaver-Burk plots: The purified enzyme 23DBDO from Comamonas sp. SMN4 had the highest catalytic activity for 2,3-dihydroxybiphenyl and 3-methylcatechol, and had very poor activity with catechol and 4-methylcatechol.

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