Isoflavone Contents, Antioxidative and Fibrinolytic Activities of Red Bean and Mung Bean

팥과 녹두의 이소플라빈 함량과 항산화 및 혈전용해 활성

  • Published : 2003.06.01

Abstract

This study was conducted to investigate the isoflavone (daidzein and genistein) contents, and the antioxidative and fibrinolytic activities of red and mung beans. Daidzein was not found in either the red or mung beans. The genistein contents of the red and mung beans were 40.7 and 27.8 mg/kg, respectively. Both samples had very high electron donating abilities and SOD-like activities. Fibrinolytic activities were not detected in the crude mung bean extract, but fibrinolytic substances were purified or activated under various pH conditions and with heat treatments. With heat treatment at 100 $^{\circ}C$ for 10 min, the specific activity was increased 4.61 fold. This study revealed that, although red and mung beans were poor in isoflavone, they could be good sources for functional products due to their strong antioxidative activities and heat- and acid-resistant proteolytic abilities.

팥과 녹두는 떡의 소나 앙금 등 주로 전분의 특성을 이용하는 형태로 소비가 제한적인 편이다. 식생활을 통해 질병의 예방 및 개선 가능성이 대두되면서 각종 식물체로부터 여러 생리활성물질의 활성탐구 및 추출에 많은 노력이 이루어지고 있으나, 대두와 같은 두류로 분류기는 팥과 녹두의 경우 이들 생리활성물질에 대한 인구는 거의 없는 실정이다. 본 연구에서는 대두에 비해 상대적으로 연구가 미진한 팥과 녹두의 생리활성 및 이소플라본 함량을 조사하였고, 그 결과는 다음과 같다. 1.팥과 녹두에서는 daidzein이 검출되지 않았고, genistein은 건조중량 1 kg당 40.7 mg과 27.8 mg이 들어 있었다. 2. 팥과 녹두의 전자공여능은 90%의 활성을 보인 팥이 84.6%의 활성을 나타낸 녹두에 비해 유의적으로 높았다. 3. 팥과 녹두의 SOD 유사활성 역시 거의 같은 수준으로 높았고, 매우 안정적이었다. 4. 혈전용해능은 팥의 경우 원액에서는 활성이 없었으나, 추출액의 pH를 6.4로 조절한 후 18시간 동안 냉장처리한 결과 비교적 큰 활성을 보였으며, pH 3.0으로 조절시 더욱 증가하였다. 녹두는 추출액을 pH 6.4와 3.0으로 조절시 모두 비활성도가 증가하였으나 pH 3.0에서는 pH 6.4보다 활성이 낮았다. 이들 시료는 36$^{\circ}C$에서 배양시 14시간까지는 반응을 거의 보이지 않다가 15시간이 지나면서 활성이 크게 나타나는 특이성을 보였다. 5. 열처리가 혈전용해활성에 미치는 영향을 비교해보면, 팥과 녹두 모두 10$0^{\circ}C$에서 10분 처리시 가장 활성이 컸고, 그 다음이 55$^{\circ}C$에서 30분, 10$0^{\circ}C$에서 30분 순으로 유사한 양상을 보였다 특히 10$0^{\circ}C$에서 10분간의 열처리시 55$^{\circ}C$에서 30분 처리한 것에 비해 혈전용해활성이 증가한 것으로 미루어 팥과 녹두의 혈전용해활성은 열에 대한 안정성이 크다고 할 수 있다. 반면 10$0^{\circ}C$에서 30분간의 과도한 열처리는 활성의 저하를 초래했다.

Keywords

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