KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidant Effect of Vitamin-C/alginate Gel-entrapped Liposome for Resistance of DHA Autoxidation

DHA의 산화방지를 위한 Vitamin C/alginate Gel-entrapped Liposome의 항산화 효과

  • 한성철 (전남대학교 물질ㆍ생물화학공학과) ;
  • 허은정 (전남대학교 물질ㆍ생물화학공학과) ;
  • 김연주 (광주보건대학 피부미용과 & 전남대학교 고분자공학과) ;
  • 이기영 (전남대학교 응용화학공학부 & 촉매연구소) ;
  • 김진철 (강원대학교 바이오 산업공학부)
  • Published : 2003.06.01
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Abstract

Antioxidant effect of reconstituted liposome was studied for application to food and cosmetic industry. Vitamin-C/alginate alginate gel-entrapped DHA-PC liposome (AVDL) was prepared and studied with respect to morphology (TEM), loading efficiency (TLC) and antioxidant effect (TBARS assay). AVDL has spherical structure and 80 ∼ 250 ㎚ size. As DHA content increased, DHA and PC loading efficiency and antioxidant effect was increased. At addition of 0.2 % vitamin C in AVDL, DHA auto-oxidation was minimized and sustained about 3 days.

PC 리포솜에 최대로 포함되는 DHA의 양은 PC : DHA의 비율이 1 : 0.8인 것으로 나타났으며 이때 적재 효율은 약 91 %였고 AVDL의 경우에는 약 17 %이었다. DHA의 산화를 방지하기 위하여 vitamin C/alginate 리포솜에 병합시킬 수 있었고 TEM을 통하여 제조된 다양한 리포솜의 형태와 크기를 확인할 수 있었다. TBARS 분석법에 의해 $40^{\circ}C$에서 DHA가 포함된 PC 리포솜과 AVDL에 포함된 DHA의 산화정도를 분석하였고, 결과적으로 자연 상태에서 리포솜에 포함된 DHA는 밀도가 높아질수록 산화에 안정하며, vitamin C를 포함한 alginate gel 리포솜은 DHA의 산화를 크게 억제한다는 것을 확인하였다. AVDL은 실험에서와 같은 산화조건에서 DHA의 산화를 초기 3일까지는 거의 완전히 억제하였고 그 이후에도 항산화 효과가 크다는 것을 확인하였다.

Keywords

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