Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Safety Evaluation of IGEs Separated and Refined from Colostrum

초유로부터 분리.정제된 IGFs의 안전성 평가에 관한 연구

  • Published : 2003.06.01
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Abstract

This study was carried out to investigate safety evaluation of IGEs separated and refined from bovine milk and commercial recombinant human IGFs. In order to evaluate toxicity of these samples, acute toxicity test and short term toxicity test were investigated with IGF-I separated and refined from colostrum and commercial recombinant human IGF-I from R&D systems company. for acute toxicity test, we selected recombinant human IGF-I from R&D systems company and establish one control group and three dose-level groups(0, 10, 20 and 50 $\mu\textrm{g}$ per rat). We have intravenously injected tail of rats with selected sample once. After 20 days, pathological cellular tissue analyses were investigated with liver, kidney and spleen of 12 rats in all test groups. However, Morbid tissue and abnormal statistical results were not discovered in all cellular tissues. For short term toxicity test, we selected IGF-I separated and refined from colostrum and establish one control group and three dose-level groups(0, 5, 10 and 15 $\mu\textrm{g}$/day per rat). Rats were orally injected with selected sample once a day during two weeks. After short term toxicity test period, Pathological cellular tissue analyses were investigate with liver, kidney and spleen of 12 rats in all test groups. However, Morbid tissue and abnormal statistical results were not discovered in all cellular tissues. These results suggest that IGF-I treated groups show no significant toxicological findings with changes of body weight, food consumption, water consumption, and pathological findings compared with control groups.

본 연구에서는 IGFs를 다양한 기능성 유제품에 적용하기 전에 먼저 상업용 IGFs 및 초유로부터 분리.정제된 IGFs의 안전성을 평가하여 새로운 기능성 소재로서의 IGFs의 가능성을 검토해 보고자 하였다. 이를 위하여 독성평가에서 기본적으로 수행되고 있는 단회투여독성시험과 반복투여독성시험을 실시하였다. 단회투여독성시험에서는 R&D Systems사의 Recombinant Human IGF- I 을 시험물질로 하고 생후 4주된 Sprague-Dawley계(♂) 흰쥐(rat)를 실험동물로 하여 개체당 0, 10, 20, 50 $\mu\textrm{g}$씩 투여하는 시험군을 설정한 후, 꼬리정 꼬리정맥에 주사하여 20일간 관찰하면서 체중변화, 식이섭취변화를 측정하고 최종적으로 부검하여 육안검사 및 간장, 신장, 비장에 대한 병리조직검사를 실시한 결과, 명확한 이상소견이 나타나지 않아 모든 시험군에서 급성독성이 확인되지 않았다. 반복투여독성시험에서는 초유로부터 분리.정제된 IGF- I 을 시험물질로 하고 생후 4주된 Sprague-Dawley계(♂) 흰쥐(rat)를 실험동물로 하여 개체당 0, 5, 10, 15 $\mu\textrm{g}$씩 투여하는 시험군을 설정한 후, 14일간 반복적으로 강제경구투여하면서 임상증상을 관찰하면서 체중변화, 식이섭취변화를 측정하였고 최종적으로 부검하여 육안검사 및 간장, 신장, 비장에 대한 병리조직검사를 실시한 결과, 명확한 이상소견이 나타나지 않아 모든 투여농도에서 반복투여독성시험을 비롯한 기본적인 독성평가에 있어서 초유 내 IGF-I의 안전성에는 명확한 이상소견이 없는 것으로 판단되었다.

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References

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