Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Characterization of Diesel Oil Degrading Bacterium, Pseudomonas sp. GENECO 1 Isolated from Oil Contaminated Soil

유류 오염 토양으로부터 분리한 디젤 분해 세균 Pseudomonas sp. GENECO 1의 분리 및 특성 규명

  • 이종광 (삼성엔지니어링 기술연구소) ;
  • 김무훈 (삼성엔지니어링 기술연구소) ;
  • 박형수 (삼성엔지니어링 기술연구소)
  • Published : 2003.06.01
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Abstract

With the enrichment culture technique, bacterial strains which degrade diesel oil were isolated from soil contaminated with diesel oil. One of the isolates named GENECO 1 showed the highest activity for emulsification of diesel oil as well as the highest growth rate. This strain, GENECO 1, was identified as a Pseudomonas sp. based on its biochemical, physiological characteristics and 16S rDNA sequences. The optimal cultural conditions for cell growth and oil emulsifying activity of its culture were as follow; $30^{\circ}C$ for temperature, 7.0 for pH. Diesel oil degradation was analysed by the gas chromatography. More than 95% of 1% treated diesel oil were converted into a form no longer extractable by mixed organic solvents after 96 hours incubation.

유류오염 토양으로부터 디젤에 대한 분해능이 있는 균주를 순수분리하였고, 이들 분리 균주의 액체 배양을통하여 균체 생육과 유화활성이 우수한 균주를 최종적으로 선별하였다. 생리,생화학적 특성 및 165 rDNA 염기서열 분석을 실시한 결과, Pseudomonas sp.로 분류 되었으며, Pseudomonas sp. GENECO 1으로 명명하였다. Bioscreen C를 이용하여 디젤 분해를 위한 배양 조건을 조사한 결과 최적 배양온도는 $30^{\circ}C$, 초기 pH는 7.0로 나타났다. Gas chromatography를 이용한 배양 시간별 잔류 디젤 성분분석 결과 96시간 이내에 1.0%의 디젤을 95%이상 분해하였다.

Keywords

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