Journal of Microbiology and Biotechnology

  • 제13권3호
  • /
  • Pages.394-399
  • /
  • 2003
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Growth Inhibition and Apoptosis Induction of Gastric Cancer Cells by Copper (II) Glycinate Complex

  • JE CHUL LEE (Department of Microbiology, Eulji University School of Medicine) ;
  • JEONG, YONG WOOK (Department of Microbiology, College of Medicine, Seonam University) ;
  • KISUNG KIM (Department of Microbiology, College of Medicine, Seonam University) ;
  • JAE YOUNG OH (Department of Microbiology, College of Medicine, Seonam University) ;
  • JONG CHUN PARK (Department of Microbiology, College of Medicine, Seonam University) ;
  • JUNG HWAN BANG (Department of Environmental Health, Seonam University) ;
  • ANG WON CHOI (Department of Chemistry, Yosu National University)
  • 발행 : 2003.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The in vitro cytotoxic effects of newly synthesized copper (II) glycinate complex were investigated in two gastric cancer cell lines of SNU484 and SNU638 cells. The complex inhibited the growth and decreased the viability of both gastric cancer cells in a dose-dependent manner. Gastric cancer tells treated with the complex exhibited the features of apoptosis, as demonstrated by fragmentation of chromosomal DNA, activation of caspase-3-like enzyme, and cleavage of poly［ADP-ribose］ polymerase (PARP). With the treatment of copper (II) glycinate complex, the active form of caspase-3 was observed in SNU484 cells, but not in SNU638 cells, indicating that an alternative pathway of apoptosis might have been triggered in SNU638 cells. In conclusion, copper (II) glycinate complex induces apoptosis of SNU484 and SNU638 gastric cancer cells, and it is suggested that novel copper (II) glycinate complex is highly active against human gastric cancer cells.

키워드

참고문헌

  1. J. Cell Biochem v.64 Mammalian cell death proteases: A family of highly conserved asparatate specific cysteine proteases Alnemri,E.S. https://doi.org/10.1002/(SICI)1097-4644(199701)64:1<33::AID-JCB6>3.0.CO;2-0
  2. Science v.281 Death receptors: Signaling and modulation Ashekanzi,A.;V.M.Dixit https://doi.org/10.1126/science.281.5381.1305
  3. J. Microbiol. Biotechnol v.11 Cytotoxic effect of urushiol on human ovarian cancer cells Choi,J.Y.;C.S.Park;J.Choi;H.Rhim;H.J.Chun
  4. J. Kor. Environ. Sci. Soc. v.9 Disinfectant effects of ecological familiar surfactant against fish pathogens Choi,S.W.;K.Y.Chung;N.H.Oh;E.Y.Kim;J.H.Bang;J.W.Kim;M.H.Yeo
  5. Trends Biochem. Sci. v.18 The control of apoptosis in mammalian cells Collins,M.K.L.;A. Lopez-Rivas https://doi.org/10.1016/0968-0004(93)90042-L
  6. Toxicol. In Vitro v.14 Induction of apoptosis by a novel copper-based anticancer compound, casiopeina Ⅱ, in L 1210 murine leukemia and DH1 human ovarian carcinoma cells De Vizcaya-Ruiz, A.;A. Rivero-Muller;L. Ruiz-Ramirez;G.E.N.Kass;L.R.Kelland;R.M.Orr;M.Dobrota https://doi.org/10.1016/S0887-2333(99)00082-X
  7. Biochem. Cell Biol. v.75 Cleavage of poly(ADP-ribose) polymerase: A sensitive parameter to study cell death Duriez,P.Z.;G.M.Shah https://doi.org/10.1139/bcb-75-4-337
  8. Nature v.375 Involvement of an ICE-like protease in Fas-mediated apoptosis Enari,M.;H.Hug;S.Nagata https://doi.org/10.1038/375078a0
  9. Nature v.380 Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis Enari,M.;R.V.Talanian;W.W.Wong;S.Nagata https://doi.org/10.1038/380723a0
  10. Drugs Exp. Clin. Res. v.24 In vitro combined effect of a new series of copper (Ⅱ) complexes with cisplatin or epirubicin on human breast and cervical cancer cell lines Geromichalos,G.D.;G.A.Katsoulos;C.C.Hadjikostas;A.H.Kortsaris;D.A.Kyriakidis
  11. Anal. Biochem. v.218 A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry Gong,J.;F.Traganos;Z.Darzynkiewilz https://doi.org/10.1006/abio.1994.1184
  12. Int. J. Oncol. v.6 Cell death by apoptosis following anticancer drug treatment in vitro Huschtscha,L.I.;W.A.Bartier;A.Malstrom;M.H.N.Tattersal
  13. J. Microbiol. Biotechnol. v.12 Antitumor activity of chitosan oligosaccharides produced in ultrafiltration membrane reactor system Jeon,Y.J.;S.K.Kim
  14. Cancer v.73 Apoptosis, its significance in cancer and cancer therapy Kerr,J.F.R.;C.M.Winterford;B.V.Harmon https://doi.org/10.1002/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO;2-J
  15. Eur. J. Clin. Pharmacol v.47 Complexes of metals other than platinum as antitumor agents Kopf-Maier, P.
  16. Cancer v.80 Alterations of p$16^ {INK4a} and p$15^ {INK4a} genes in gastric carcinomas Lee,Y.Y.;S.H.Kang;J.Y.Seo;C.W.Jung;K.U.Lee;K.J.Choe;B.K.Kim;N.K.Kim;H.P.Koeffler:Y.J.Bang https://doi.org/10.1002/(SICI)1097-0142(19971115)80:10<1889::AID-CNCR3>3.0.CO;2-J
  17. APMIS v.109 Apoptotic cell death induced by Acinetobacter baumannii in epithelial cells through caspase-3 activation Lee,J.C.;J.Y.Oh;K.S.Kim;Y.W.Jeong;J.C.Park;J.W.Cho https://doi.org/10.1034/j.1600-0463.2001.d01-132.x
  18. J. Kor. Cancer Assoc. v.32 Expression of cell cycle control genes in Korean gastric cancer cell lines Lim,C.C.;I.H.Kim;J.W.Cho;W.K.Baek;S.I.Suh;M.H.Suh;J.W.Park;J.S.Kang;S.S.Sohn
  19. Cell v.86 Induction of apoptotic program in cell-free extracts: Requirement for dATP and cytochrome C Liu,X.;C.N.Kim;J.Yang;R.Jemmerson;X.Wang https://doi.org/10.1016/S0092-8674(00)80085-9
  20. Cell v.88 Apoptosis by death receptor Nagata,S. https://doi.org/10.1016/S0092-8674(00)81874-7
  21. Nature v.376 Identification and inhibition of ICE/CED-3 protease necessary for mammalian apoptosis Nicholson,D.W.;A.Ali;N.A.Thornsberry;J.P.Vaillantcourt;C.K.Ding;M.Gallant;Y.Careau;P.R.Griffin;M.Labelle;Y.A.Lazebnik;N.A.Munday;S.M.Raju;M.E.Smulson;T.T.Yamin;V.L.Yu;D.K.Miller https://doi.org/10.1038/376037a0
  22. Br. J. Cancer v.69 The role of apoptosis in cell killing by cisplatin: A flow cytometric study Ormerod,M.G.;R.M.Orr;J.H.Peacock https://doi.org/10.1038/bjc.1994.14
  23. Curr. Opin. Oncol. v.11 Mechanisms of apoptosis avoidance in cancer Reed,J.C. https://doi.org/10.1097/00001622-199901000-00014
  24. J. Inorg. Biochem. v.51 Cytostatic, mutagenic, antineoplastic activities and preliminary toxicity of copper (Ⅱ) new drugs: Casiopeinas Ⅰ, Ⅱ, Ⅲ Ruiz-Ramirez, L;I. Gracia-Mora;M.E. De La Rosa;H.Sumano;C.Gomez;F.Arenas;E.Gomez;E.Pimentel;M.Cruces https://doi.org/10.1016/0162-0134(93)85435-B
  25. Cell v.91 Caspases: Intracellular signaling by proteolysis Salvesen,G.S.;V.M.Dixit https://doi.org/10.1016/S0092-8674(00)80430-4
  26. J. Gastroenterol v.36 Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides bunge Takeda,Y.;H.Togashi;T.Matsuo;H.Shinzawa;Y.Takeda;T.Takahashi https://doi.org/10.1007/s005350170135
  27. Exp. Cell Res. v.219 Cellular events in Fas/APO-1 mediated apoptosis in JURKAT T lymphocytes Weis,M.;J.Schlegel;G.E.N.Kass;T.H.Holmstrom;I.Peters;J.E.Eriksson;S.Orrenius;S.C.Chow https://doi.org/10.1006/excr.1995.1281