Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Bacillus alcalophilus AX2000 Producing Alkaling Xylanase and Its Enzyme Production

알칼리성 Xylanase를 생산하는 Bacillus alcalojnhilus AX2000의 분리와 효소 생산

  • 박영서 (경원대학교 공과대학 생명공학부) ;
  • 김태영 (경원대학교 공과대학 생명공학부)
  • Published : 2003.06.01
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Abstract

An alkali-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus alcaiophilus. This strain, named B. alcalophilus AX2000, was able to grow and produce xylanase optimally at pH 10.5 and $37^{\circ}C$. The maximum xylanase production was obtained when 0.5%(w/v) birchwood xylan and 0.5%(w/v) polypeptone and yeast extract were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression by glucose in the culture medium, and inhibited in the presence of high concentration of xylose. The maximum activity of xylanase was observed at pH 10.0 and $50^{\circ}C$ and the enzyme activity remained was over 80% at $60^{\circ}C$ and from pH 5.0 to 11.0.

알칼리성 xylanase를 생산하는 균주를 토양으로부터 분리한 후 동정을 실시한 결과 Bacillus alcaiophilus으로 판명되었다. B. alcalophilus AX2000으로 명명한 본 균주는 pH 10.5에서 생육이 가장 좋았으며 효소활성도 가장 높았고 배지 중에 탄소원과 질소원으로서 0.5%(w/v) birchwood xylan과 0.5%(w/v) polypeptone/yeast extract를 각각 사용하였을 경우에 최대의 xylanase생산성을 나타내었다. Xylanase의 생합성근 glucose에 의한 catabolite repression을 받았으며 고농도의 xylose에 의해 효소의 생합성이 저해되었다. 조효소의 최적활성은 pH 10과 $50^{\circ}C$에서 나타났으며, pH 5에서 11까지의 넓은 pH범위에서 활성이 안정하게 유지되었고 효소의 열 안정성은 20~$60^{\circ}C$에서 30분간 처리시 90%이상의 잔존활성을 나타내었다.

Keywords

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