Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Hepatic Response in Cytochrome P45O and De-alkylase Activity of Olive Flounder (Paralichthys olivaceus) Exposed to Water-borne Phenanthrene

  • Jee, Jung-Hoon (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Park, Dae-Kuk (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
  • Published : 2003.05.01
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Abstract

Olive flounder (Paralichthys olivaceus) was exposed to water-borne phenanthrene for 4 weeks. After the exposure for 2-weeks, hepatic cytochrome P450 contents were significantly elevated. Induction of hepatic ethoxy resorufin-O-deethylase (PROD) activity was significantly increased in flounders treated with 1.0 and 2.0 $\mu$M phenanthrene, compared to untreated group and 0.5 $\mu$M treated group. However, there were no significant changes in pentoxyresorufin-O-deethylase (PROD) activity in hepatic microsome of all the phenanthrene-treated groups, compared to the untreated group. Phenanthrene has the potential to induce cytochrome P450 and EROD enzyme of the olive flounder.

Keywords

References

  1. Nova Scotia. Mar. Environ. Res. v.37 Liver microsornal mono-oxygenase induction in winter flounder (Pseudopleuronectes americanus) from a gradient of sediment PAH concentrations at Sydney Harbour Addison, R. F.;D. E. Willis;M. E. Zinck https://doi.org/10.1016/0141-1136(94)90055-8
  2. Mar. Biol. v.27 Characteristics of disperisons and water soluble extracts of curde and refined oils and their toxicity to estuarine crustaceans and fish Anderson, J. W.;J. M. Neff;B. A. Cox;H. E. Tatem;G. M. Hightower https://doi.org/10.1007/BF00394763
  3. Standard methods for the examination of water and waste water(16th edition) American water works association, and water pollution control federation APHA(American Public Health Association
  4. Polynuclear Aromatic Hydrocarbosns: Physical and Biological Chemistry Baumann, P. C.;W. D. Smith;M. Ribbick
  5. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the priniple of protein-dye binding Bradford, M. https://doi.org/10.1016/0003-2697(76)90527-3
  6. Mar. Environ. Res. v.34 Metabolic alteration of PCB residues in aquatic fauna: istributions of cytochrome P4501A1- and P4502B-like activities Brown, J. F. https://doi.org/10.1016/0141-1136(92)90118-6
  7. Environ. Toxicol. Chem. v.14 A field evaluation of cytochrome P4501A as a biomarker of contaminant expousure in three species of flat-fish Collier, T. K.;B. F. Anulacion;J. E. Stein;A. Goksoyr;U. Varanasi https://doi.org/10.1897/1552-8618(1995)14[143:AFEOCP]2.0.CO;2
  8. Comp. Biochem. Physiol. v.102C The effects of acute exposure to the water soluble fraction of diesel fuel oil on survival and metabolic rate of an antarctic fish (Pagothenia borchgrevinki) Davison, W.;C. E. Franklin;J. C. McKenzie;M. R. Dougan
  9. Biometrics v.11 Multiple-range and multiple F tests Duncan, D. B.
  10. Fish. Wild. serv. Biol. Rep. v.104C Polycyclic aromatic hydrocarbons hazards to fish, wildife,and invertebrates: a synoptic review Eisler, R.
  11. Aquat. Toxicol. v.1 Hydromineral balance and gill morphology in rainbow trout Salmogairdneri, acclimated to fresh and sea water, as affected by petroleum exposure Engelhardt, F. R.;M. P. Wong;M. E. Duey https://doi.org/10.1016/0166-445X(81)90013-8
  12. Fundulus grandis Baird and Girard. Environ. Pollut. v.14 The effects of the water-soluble fractions of No. 2 fuel oil on the early development of the estuarine fish Ernst, V. V.;J. M. Neff;J. W. Anderson
  13. Aquat. Toxicol. v.22 The cytochrome P450 system in fish, aquatic toxicology and environmental monitoring Goksoyr, A.;L. Forlin https://doi.org/10.1016/0166-445X(92)90046-P
  14. Toxicocl. Appl. Pharmacol. v.98 Cloned rainbow trout liver P 450 complementary DNA as a potential environmental monitor Haasch, M. L.;P. J. Wejksonora;J. J. Stegeman;J. J. Lech https://doi.org/10.1016/0041-008X(89)90240-8
  15. Anal. Biochem. v.222 Simultaeous meausrement of cytochrome P4501A catalytic activity and total protein concentration with a fluorescence plate reader Kennedy, S. W.;S. P. Jones https://doi.org/10.1006/abio.1994.1476
  16. Arch. Toxicol. v.39 Metabolic activation of polycyclic hydrocarbons Lehr, R. E.;D. M. Jerina https://doi.org/10.1007/BF00343269
  17. Pharmacological Reviews v.31 Metabolic activation of polycyclic hydrocarbons Lehr, R. E.;D. M. Jerina
  18. Washington. Environ. Sci. Technol. v.18 Chemical pollutants in sediments and diseases in bottom-dwelling fish in Pudged Sound Malins, D. C.;B. B. McCain;W. D. Brown;S. L. Chan;M. S. Myers;J. T. Landahl;P. G. Prohaska;A. J. Friedman;L. D. Rhodes;D. G. Burrows;W. D. Gronlund;H. O. Hodgins https://doi.org/10.1021/es00127a013
  19. Carcinogenesis v.6 Toxic chemical in sediments and biota from a creosote-polluted harbour: relationship with hepatic neoplasms and other hepatic lesions in English sole (Parophrys vetulus) Malins, D. C.;M. M. Krahn;M. S. Myers;L. Rhodes;W. D. Brown;C. A. Krone;B. B. McCain;S. Chan https://doi.org/10.1093/carcin/6.10.1463
  20. Metabolism of Polycyclic Aromatic Hydrocarbons in the Aquatic Environment Bioavailability of polycyclic aromatic hydrocarbons in the aquatic environment McElroy, A. E.;J. W. Farrington;J. M. Teal;U. Varanasi(ed.)
  21. J. Fish. Sci. Tech. v.4 Monitoring of polycyclic aromatic hydrocarbon in sediments and organsisms from Korean coast Moon, H. B.;H. G. Choi;S. S. Kim;S. R. Jeong;P. Y. Lee;G. Ok
  22. Sci. Total. Environ. v.94 Overview of studies on liver carcinogenesis in English sole from Pudget Sound; evidence for a xenobiotic chemical etiology. Pathology and epizootiology Myers, M. S.;J. T. Landahl;M. M. Krahn;L. L. Johnson;B. B. McCain https://doi.org/10.1016/0048-9697(90)90363-Y
  23. Oil in the seainputs, fates and effects NASA(National Academy of Science)
  24. J. Biol. Chem. v.239 The carbon monoxide-binding pigment of liver microsomes. II. Solubilization, Purification and properties Omura, T.;R. Sato
  25. Arch. Environ. Contam. Toxicol. v.20 Response of xenobiotic metabolizing enzymes of rainbow trout (Oncorhynchus mykiss) to the mono-ortho substituted polychlorinated PCB congener 2, 3', 4, 4', 5-pentachlorobiphenyl, PCB-118, detected by enzyme activities and immunochemical methods Skaare, J. U.;E. G. Jensen;A. Goksoeyr;E. Egaas https://doi.org/10.1007/BF01064401
  26. Biochem. Soc. Trans. v.18 Structure, function and regulation of cytochrome P450 forms in fish Stegeman, J. J.;B. R. Woodlin;R. M. Smolowitz https://doi.org/10.1042/bst0180019
  27. Office of Health and Environmental Assessment Drinking Water Criteria Document for Polycyclic Aromatic Hydrocarbons (PAHs). Prepared by the Environmental Criteria and Assessment Office U.S. EPA(U.S. Environmemtal Protection Agency)
  28. Aquat. Toxicol. v.17 Induced cytochrome P-450 in intestine and liver of spot (Leiostomus xanthurus) from a polycyclic aromatic hydrocarbon contaminated environment van Veld, P. A.;D. J. Westbrook;B. R. Woodin;R. C. Hale;C. L. Smith;R. J. Hugget;J. J. Stegeman https://doi.org/10.1016/0166-445X(90)90026-L
  29. Crit. Rev. Toxicol. v.22 The human hepatic cytochrome P-450 involved in drug metabolism Wrighton, S. A.;J. C. Stevens https://doi.org/10.3109/10408449209145319