Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Secretory Production of hGM-CSF with a High Specific Biological Activity by Transgenic Plant Cell Suspension Culture

  • Kwon, Tae-Ho (Basic Science Research Institute, Chonbuk National University) ;
  • Shin, Young-Mi (Division of Biological Sciences, Chonbuk National University) ;
  • Kim, Young-Sook (Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Jang, Yong-Suk (Bank for Cytokine Research, Chonbuk National University) ;
  • Yang, Moon-Sik (Division of Biological Sciences, Chonbuk National University)
  • Published : 2003.04.01
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Abstract

The human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene was introduced into tobacco plants. The cell suspension culture was established from leaf-derived calli of the transgenic tobacco plants in order to express and secrete a biologically active hGM -CSF. The recombinant hGM-CSF from the transgenic plant cell culture (prhGM-CSF) was identified as a yield of about 180 ${\mu}$g/L in the culture filtrate, as determined by ELISA. The addition of 0.5 g/L polyvinylpyrrolidone (PVP) to the plant cell culture medium both stabilized the secreted prhGM-CSF and increased the level of production approximately 1.5-fold to 270 ${\mu}$g/L. The biological activity of the prhGM-CSF was confirmed by measuring the proliferation of the hGM-CSF-dependent cell line, TF-1. Interestingly, the specific activity of the prhGM-CSF was estimated to be approximately 2.7 times higher than that of a commercially available preparation from E. coli.

Keywords

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