The Selective Visualization of Lignin Peroxidase, Manganese Peroxidase and Laccase, Produced by White Rot Fungi on Solid Media

  • Ryu, Won-Youl (School of Chemical Engineering & Technology, Yeungnam University) ;
  • Jang, Moon-Yup (School of Chemical Engineering & Technology, Yeungnam University) ;
  • Cho, Moo-Hwan (School of Chemical Engineering & Technology, Yeungnam University)
  • Published : 2003.04.01

Abstract

A visual method for the selective screen Eng of lignin degrading enzymes, produced by white rot fungi (WRF), was investigated by the addition of coloring additives to solid media. Of the additives used in the enzyme production media, guaiacol and RBBR could be used for the detection of lignin peroxidase (LiP), manganese peroxidase (MnP) and lactase. Syringaldazine and Acid Red 264 were able for the detection of both the MnP and lactase, and the LiP and laccase, respectively, and a combination of these two additives was able to detect each of the ligninases produced by the WRF on solid media.

Keywords

References

  1. Science v.221 Lignin-degrading enzyme from hymenimycete Phanerochaete chrysosporium Tien,M.;T.K.Kirk https://doi.org/10.1126/science.221.4611.661
  2. FEMS Microbiol. v.13 Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation Hatakka,A. https://doi.org/10.1111/j.1574-6976.1994.tb00039.x
  3. Enzyme Microb. Technol. v.18 Laccase catalyzes formation of an indamine dye between 3-methyl-2-benzothiazolinone hydrazone and 3-dimethylaminobenzoic acid Baker,W.L.;K.Sabapathy;M.Vibat;G.Lonergan https://doi.org/10.1016/0141-0229(95)00074-7
  4. Appl. Environ. Microbiol. v.62 Mineralization of polycyclic aromatic hydrocarbons by the white rot fungus Pleurotus ostreatus Bezarel,L.;Y.Hadar;C.E.Cerniglia
  5. Appl. Environ. Microbiol. v.54 Laccase-mediated detoxification of phenolic compounds Bollag,J.M.;K.L.Shuttleworth;D.H.Anderson
  6. Science v.228 Oxidation of persistent environmental pollutants by a white rot fungus Bumpus,J.A.;M.Tien;D.Wright;S.Aust https://doi.org/10.1126/science.3925550
  7. Appl. Environ. Microbiol. v.58 Biodegradation of crystal violet by the white rot fungus Phanerochaete chrysosporium Bumpus,J.A.;B.Brock
  8. Appl. Environ. Microbiol. v.44 Metabolism of 7-methyl-benzo[a]anthracene and 7-hydroxymethylbenz[a]anthracene by Cunninghamella alegance Cerniglia,C.E.;P.P.Fu;S.K.Yang
  9. Appl. Environ. Microbiol. v.62 Oxidation of anthracene and benzo[a]pyrene by laccase from Trametes versicolor Collins,P.J.;M.J.J.Kotterman;J.A.Field;A.D.W.Dobson
  10. Korean J. Biotech. Bioeng. v.15 no.3 Biodegradation of polycyclic aromatic hydrocarbons by white rot fungi Ryu,W.R.;Y.S.Seo;Y.K.Chang;M.H.Cho
  11. Appl. Environ. Microbiol. v.45 Decolorization of several polymeric dyes by the lignin-degrading basidiomycete Phanerochaete chrysosporium Glenn,J.K.;M.H.Gold
  12. Appl. Environ. Microbiol. v.58 Influence of aromatic substitution pattern on azo dye degradability by Streptomyces spp. and Phanerochaete chrysosporium Pasti-Grigsby,M.B.;A.Paszczynski;S.Goszczynski;D.L.Crawford;R.L.Crawford
  13. Appl. Biochem. Biotechnol. v.51 Use of dyes in solid medium for screening ligninolytic activity of selective actinomycetes Chahal,D.S.;D.Kluepfel;R.Morosoli;F.Shareck;S.Laplante;D.Rouleau https://doi.org/10.1007/BF02933418
  14. Methods Enzymol. v.161 Lignin peroxidase of Phanerochaete chrysosporium Tien,M.;T.K.Kirk https://doi.org/10.1016/0076-6879(88)61025-1
  15. Biotechnol. Bioprocess Eng. v.5 Biodegradation of pentachlorophenol by white rot fungi under ligninolytic and nonligninolytic conditions Ryu,W.R.;S.H.Shim;M.Y.Jang;Y.J.Jeon;K.K.Oh;M.H.Cho https://doi.org/10.1007/BF02936597
  16. Korean. J. Biotechnol. Bioeng. v.15 Biodegradation of polycyclic aromatic hydrocarbons using immobilized cells of Phanerochaete chrysosporium Seo,Y.S.;W.R.Ryu;Y.K.Chang;M.H.Cho
  17. Appl. Environ. Microbiol. v.59 Purification and characterization of phenoloxidase(Laccase) from the lignin-degrading basidiomycete PM1 Pedro,M.;J.M.Coll
  18. Appl. Environ. Microbiol. v.61 Production and characterization of Trametes versicolor mutants unable to bleach hardwood kraft pulp Addleman,K.;T.Dumonceaux;M.G.Paice;R.Bourbonnais;F.S.Archibald
  19. Appl. Environ. Microbiol. v.59 Decolorization of azo, triphenyl methane, heterocyclic, and polymeric dyes by lignin peroxidase isoenzymes from Phanerochaete chrysosporium Ollikka,P.;K.Alhonmaki;V.M.Lepparen;T.Glumoff;T.Raijola;I.Suominen
  20. Biotechnol. Lett. v.18 Laccase variation during dye decolorization in a 200 L packed-bed bioreactor Schliephake,K.;G.T.Lonegran https://doi.org/10.1007/BF00154614
  21. Appl. Environ. Microbiol. v.61 Involvement of extracellular $H_2O_2$-dependent ligninolytic activity of the white rot fungus Pleurotus ostreatus in the decolorization of Remazol Brilliant Blue R Vyas,B.R.M.;H.P.Molitoris
  22. Microbiology v.140 The structure and function of fungal laccases Thurston,C.F. https://doi.org/10.1099/13500872-140-1-19