Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Carotenoids Biosynthesis and Their Metabolic Engineering in Plants

식물에서 Carotenoid 생합성 경로와 대사공학적 응용

  • 하선화 (농업생명공학연구원 신기능소재개발과) ;
  • 김정봉 (농업생명공학연구원 신기능소재개발과) ;
  • 박종석 (농업생명공학연구원 신기능소재개발과) ;
  • 류태훈 (유전자제어공학과) ;
  • 김경환 (농업생명공학연구원 신기능소재개발과) ;
  • 한범수 (농업생명공학연구원 신기능소재개발과) ;
  • 김종범 (농업생명공학연구원 신기능소재개발과) ;
  • 김용환 (농업생명공학연구원 신기능소재개발과)
  • Published : 2003.03.01
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Abstract

Carotenoids are synthesized from the plastidic glyceraldehyde-3-phosphate (GAP)/pyruvate pathway in isoprenoids biosynthetic system of plants. They play a crucial role in light harvesting, work as photoprotective agents in photosynthesis of nature, and are also responsible for the red, orange and yellow colors of fruits and flowers in plants. In addition to biological actions of carotenoids as antioxidants and natural pigments, they are essential components of human diet as a source of vitamin A. It has been also suggested that some kinds of carotenoids might provide protection against cancer and heart disease as human medicines. In this article, we review the commercial applications on the basis of biological functions of carotenoids, summarize the studies of genes involved in the carotenoid biosynthetic pathway, and introduce recent results achieved in metabolic engineering of carotenoids. This effort for understanding the carotenoids metabolism will make us to increase the total carotenoid contents of crop plants, direct the carotenoid biosynthetic machinery towards other useful carotenoids, and produce a new array of carotenoids by further metabolizing the new precursors that are created when one or two key enzymes in carotenoid biosynthetic pathway are exchanged through gene manipulation in the near future.

Keywords

References

  1. AI-Babili S, Hugueney P, Schledz M, Welsch R, Frohnmeyer H, Laule O, Beyer P (2000) Identification of a novel gene coding for neoxanthin synthase from Solanum tuberosum. FEBS Lett 485: 168-172 https://doi.org/10.1016/S0014-5793(00)02193-1
  2. AI-Babili S, Lintig J, Kleinig H, BeyerP (1996) A novel soluble form of phytoene desaturase from Narcissus pseudotuucissus chromoplasts is Hsp70-complexed and competant for flavinglation, membrane association and enzymatic activation. Plant J 9: 601-612 https://doi.org/10.1046/j.1365-313X.1996.9050601.x
  3. Araki N, Kusumi K, Niwa Y, Iba K (2000) Temperature-sensitive Arabidopsis mutant defective in 1-deoxy-D-xylulose-5-phosphate synthase within the plastid non-mevalonate pathway of isoprenoid biosynthesis. Physiol Plant 108: 19-24
  4. Arigoni D, Sagner S, Latzel C, Eisenreich W, Bacher A, Zenk MH (1997) Terpenoid biosynthesis from 1-deoxy-D-xylulose in higher plants by intramolecular skeletal rearrangement. Proc Natl Acad Sci USA 94: 10600-10605 https://doi.org/10.1073/pnas.94.20.10600
  5. Ausich RL, Brinkhaus FL, Mukharji I, Proffitt JH, Yarger JG, Yen HB (1993a) Beta-carotene biosynthesis in genetically engineered hosts. US patent 5530188
  6. Ausich RL, Brinkhaus FL, Mukharji I, Proffitt JH, Yarger JG, Yen HB (1993b) Biosynthesis of zeaxanthin and glycosylated zeaxanthin in genetically engineered hosts. US patent 5684238
  7. Bartley GE, Scolnik PA (1993) cDNA cloning, expression during development and genome mapping of PSY2, a second tomato gene encoding phytoene synthase. J Bioi Chem 268: 25718-25721
  8. Bartley GE, Scolnik PA (1995) Plant carotenoid: pigments for photoprotection, visual attraction, and human health. Plant Cell 7: 1027-1038 https://doi.org/10.1105/tpc.7.7.1027
  9. Bartley GE, Scolnik PA, Giuliano G (1994) Molecular biology of carotenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Bioi 45: 287-301 https://doi.org/10.1146/annurev.pp.45.060194.001443
  10. Bartley GE, Vitanen PV, Bacot KO, Scolnik PA (1992) A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway. J Bioi Chem 267: 5036-5039
  11. Bartley GE, Vitanen PV, Peeker I, Chamovitz D, Hirschberg J, Scolnik PA (1991) Molecular cloning and expression in photosynthetic bacteria of a soybean cDNA coding for phytoene desaturase, an enzyme of the carotenoid biosynthesis pathway. Proc Natl Acad Sci USA 88: 6532-6536 https://doi.org/10.1073/pnas.88.15.6532
  12. Bird CR, Grierson D, Schuch W (1994) Process for modifying the production of carotenoids in plants, and DNA, constructs and cells therefor. US patent 575086
  13. Bird CR, Ray JA, Fletcher JD, Boniwell JM, Bird AS, Teulieres C, Blain I, Bramley PM, Schuch W (1991) Using antisense RNA to study gene function: inhibition of carotenoid biosynthesis in transgenic tomatoes. Bio/Technoloqy 9: 635-639 https://doi.org/10.1038/nbt0791-635
  14. Bonk M, Hoffmann B, Von Untig J, Schledz M, AI-Babili S, Hobeika E, Kleinig H, Beyer P (1997) Chloroplast import of four carotenoid biosynthetic enzymes in vitro reveals differential fates prior to membrane binding and oligomeric assembly. Eur J Biochem 247: 942-950 https://doi.org/10.1111/j.1432-1033.1997.00942.x
  15. Bouvier F, Backhaus RA, Camara B (1998) Induction and control of chromoplast-specific carotenoid genes by oxidative stress. J Bioi Chem 273: 30651-30659 https://doi.org/10.1074/jbc.273.46.30651
  16. Bouvier F, Camara B, Kuntz M (2000) Elevation of the provitamin A content of transgenic tomato plants. Nat Biotechnol 18: 666-669 https://doi.org/10.1038/76523
  17. Bouvier F, d'Harlingue A, Backhaus RA, Kumagai MH, Camara B (2000) Identification of neoxanthin synthase as a carotenoid cyclase paralog. Eur J Biolchem 267: 6346-6352 https://doi.org/10.1046/j.1432-1327.2000.01722.x
  18. Bouvier F, d'Harlingue A, Hugueney P, Marin E, Marionpoll A, Camara B (1996) Xanthophyll biosynthesis-cloning, expression, functional reconstitution, and regulation of beta-cyclohexenyl carorenoid epoxidase from pepper (Capsicum annuum). J BioI Chem 271: 28861-28867 https://doi.org/10.1074/jbc.271.46.28861
  19. Bouvier F, d'Harlingue A, Suire C, Backhaus RA, Camara B (1998) Dedicated roles of plastid transketolases during the early onset of isoprenoid biogenesis in pepper fruits. Plant Physiol 117: 1423-1431 https://doi.org/10.1104/pp.117.4.1423
  20. Bouvier F, Hugueney P, d'Harlingue A, Kuntz M, Camara B (1994) Xanthophyll biosynthesis in chromoplasts: isolation and molecular cloning of an enzyme catalyzing the conversion of 5, 6-epoxycarotenoid into ketocarotenoid. Plant J 6: 45-54 https://doi.org/10.1046/j.1365-313X.1994.6010045.x
  21. Bouvier F, Keller Y, d'Hariingue A, Camara B (1998) Xanthophyll biosynthesis: Molecular and functional characterization of carotenoid hydroxylase from pepper fruits. Biochim Biophys Acta 1391: 320-328 https://doi.org/10.1016/S0005-2760(98)00029-0
  22. Bramley P, Teulieres C, Blain I, Brid C, Schuch W (1992) Biochemical characterization of transgenic tomato plants in which carotenoid synthesis has been inhibited through the expression of antisense RNA to pTOM5. Plant J 2: 343-349 https://doi.org/10.1111/j.1365-313X.1992.00343.x
  23. Bugos RC, Hieber AD, Yamamoto HY (1998) Xanthophyll cycle enzymes are members of the Iipocalin family, the first identified from plants. J Biol Chem 273: 15321-15324 https://doi.org/10.1074/jbc.273.25.15321
  24. Bugos RC, Yamamoto HY (1996) Molecular cloning of violaxanthin de-epoxidase from romaine lettuce and expression in Escherichia coli. Proc Natl Acad Sci USA 93: 6320-6325 https://doi.org/10.1073/pnas.93.13.6320
  25. Burbidge A, Grieve T, Terry C, Corlett J, Thompson A, Taylor I (1997) Structure and expression of a cDNA encoding zeaxanthin epoxidase, isolated from a wilt-related tomato (Lycopersicon esculentum Mill.). J Exp Bot 48: 1749-1750
  26. Burkhardt PK, Beyer P, Wunn J, Kloti A, Amstrong GA, Schledz M, von Linting J, Potrykus I (1997) Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulatesphytoene, a key intermediate of provitamin A biosynthesis. Plant J 11: 1071-1078 https://doi.org/10.1046/j.1365-313X.1997.11051071.x
  27. Chahed K, Oudin A, Guivarc'h N, Hamdi S, Chenieux JC, Rideau M, Clastre M (2000) 1-Deoxy-D-xylulose 5-phosphate synthase from periwinkle: cDNA identification and induced gene expression in terpenoid indole alkaloid-producing cells. Plant Physiol Biochem 38: 559-566 https://doi.org/10.1016/S0981-9428(00)00781-6
  28. Corona V, Aracri B, Kosturkova G, Bartley GE, Pitto L, Giorgetti L, Scolnik PA, Giulano G (1996) Regulation of a carotenoid biosynthesis gene promoter during tomato development. Plant J 9: 505-512 https://doi.org/10.1046/j.1365-313X.1996.09040505.x
  29. Cunningham FX Jr, Chamovitz D, Misawa N, Gantt E, Hirschberg J (1993) Cloning and functional expresion in Escherichia coli of a cyanobacterial gene for Iycopene cyclase, the enzyme that catalyzes the biosynthesis of $\beta$-carotene. FEBS Lett 328: 130-138 https://doi.org/10.1016/0014-5793(93)80980-9
  30. Cunningham FX Jr, Gantt E (1998) Genes and enzymes of carotenoid Biosynthesis in plants. Annu Rev Plant Physiol Plant Mol BioI 49: 557-583 https://doi.org/10.1146/annurev.arplant.49.1.557
  31. Cunningham FX Jr, Gantt E (2001) One ring or two? Determination of ring number in carotenoids by Iycopene $\epsilon$-cyclases. Proc Natl Acad Sci USA 98: 2905-2910 https://doi.org/10.1073/pnas.051618398
  32. Cunningham FX Jr, Pogson B, Sun Z, McDonald KA, DellaPenna D, Gantt E (1996) Functional analysis of the beta and epsilon Iycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8: 1613-1626 https://doi.org/10.1105/tpc.8.9.1613
  33. Deruere J, Bouvier F, Steppuhn J, Klein A, Camara B, Kuntz M (1994) Structure and expression of two plant genes encoding chromoplast-specific proteins: occurrence of partially spliced transcripts. Biochem Biophys ResCommun 199: 1144-1150 https://doi.org/10.1006/bbrc.1994.1350
  34. Deruere J, RomerS, d'Harlingue A, Backhaus RA, Kuntz M, Camara B (1994) Fibril assembly and carotenoid overaccumulation in chromoplasts: a model for supermolecular lipoprotein structure. Plant Cell 6: 119-133 https://doi.org/10.1105/tpc.6.1.119
  35. Eisenreich W, Rohdich F, Bacher A (2001) Deoxyxylulose phosphate pathway to terpenoids. Trends Plant Sci 6: 78-84 https://doi.org/10.1016/S1360-1385(00)01812-4
  36. Estevez JM, Cantero A, Romero C, Kawaide H, Jimenez LF, Kuzuyama T, Seto H, Kamiya Y, Leon P (2000) Analysis of the expression of CLA1, a gene that encodes the 1-deoxyxylulose 5phosphate synthase of the 2C-methyl-D-erythritol-4-phosphate pathway in Arabidopsis. Plant Physiol 124: 95-103 https://doi.org/10.1104/pp.124.1.95
  37. Fraser PD, Romer S. Shipton CA, Mills PB, Kiano JW, Misawa N, Drake RG, Schuch W, Bramley PM (2002) Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner. Proc Natl Acad Sci USA 99: 1092-1097 https://doi.org/10.1073/pnas.241374598
  38. Fray RG, Wallace A, Fraster PD, Valero D, Hedden P, Bramley PM, Grierson D (1995) Constitutive expression of a fruit phytoene synthase gene in transgenic tomatoes causes dwarfism by redirecting metabolites from the gibberellin pathway. Plant J 8: 693-701 https://doi.org/10.1046/j.1365-313X.1995.08050693.x
  39. Giuliano G (2000) Metabolic engineering of beta-carotene and Iycopene content in tomato fruit. Plant J 24: 413-420 https://doi.org/10.1046/j.1365-313x.2000.00880.x
  40. Giuliano G, Aquilani R, Dharmapuri S (2000) Metabolic engineering of plant carotenoids. Trends Plant Sci 5: 406-409 https://doi.org/10.1016/S1360-1385(00)01749-0
  41. Giuliano G, Bartley GE, Scolnik PA (1993) Regulation of carotenoid biosynthesis during tomato development. Plant Cell 5: 379 387 https://doi.org/10.1105/tpc.5.4.379
  42. Gracia-Asua G, Lang HP, Cogdell RJ, Hunter CN (1998) Carotenoid diversity: a molecular role for the phytoene desaturase step. Trends Plant Sci 3: 445-449 https://doi.org/10.1016/S1360-1385(98)01329-6
  43. Ha SH, Lee SW, Kim JG, Hwang YS (1999) The expression of genes involved in carotenoid biosynthesis in pepper. Agric Chem Biotechnol 42: 92-96
  44. Hauptmann R, Eschenfeldt WH, English J, Brinkhaus FL (1994) Enhanced carotenoid accumulation in storage organs of genetically engineered plants. US patent 5618988
  45. Herz S, Wungsintaweekul J, Schuhr CA, Hecht S, Luttgen H, Sanger S, Fellermerier M, Eisenreich W, ZenkMH, Bacher A, Rohdich F (2000) Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate. Proc Natl Acad Sci USA 97: 2486-2490 https://doi.org/10.1073/pnas.040554697
  46. Hirschberg J (1998) Molecular Biology of Carotenoid Biosynthesis. In: Britton G, Liaaen-Jensen S, Pfander H (eds), Carotenoids Vol.3 Biosynthesis and Metabolism, Birkhaeuser Verlag, Basel, Boston, Berlin, pp 149-194
  47. Hirschberg J (2001) Carotenoid biosynthesis in flowering plants. Curr Opin Plant Bioi 4: 210-218 https://doi.org/10.1016/S1369-5266(00)00163-1
  48. Hugueney P, Badililo A, Chen HC, Klein A, Hirschberg J, Camara B, Kuntz M(1995) Metabolism of cyclic carotenoids: a model for the alteration of this biosynthetic pathway in Capsicum annuum chromoplasts. Plant J 8: 417-424 https://doi.org/10.1046/j.1365-313X.1995.08030417.x
  49. Hugueney P, Romer S, Kuntz M, Camara B (1992) Characterization and molecular cloning of a flavoprotein catalyzing the synthesis of phytofluene and zeta-carotene in Capsicum chromoplasts. Eur J Biochem 209: 399-407 https://doi.org/10.1111/j.1432-1033.1992.tb17302.x
  50. Kumagai MH, Donson J, della-Cioppa G, Harvey D, Hanley K, Grill LK (1995) Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci USA 92: 1679-1683 https://doi.org/10.1073/pnas.92.5.1679
  51. Kumagai MH, Keller Y, Bouvier F, Clary D, Camara B (1998) Functional interaction of non-native carotenoids into chloroplasts by viral-derived expression of capsanthin-capsorubin synthase in Nicotiana benthamiana. Plant J 14: 305-315 https://doi.org/10.1046/j.1365-313X.1998.00128.x
  52. Kunz M, Romer S, Hugueney P, Weil JH, Schantz R, Camara B (1992) Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening. Plant J 2: 25-34
  53. Lange BM, CroteauR (1999a) Isoprenoid biosynthesis via a mevalonate independent pathway in plants: cloning and heterologous expression of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from peppermint. Arch Biochem Biophys 365: 170-174 https://doi.org/10.1006/abbi.1999.1168
  54. Lange BM, Croteau R (1999b) Isopentenyl diphosphate biosynthesis via a mevalonate-independent pathway: isopentenyl monophosphate kinase catalyses the terminal enzymatic step. Proc Natl Acad Sci USA 96: 13714-13719 https://doi.org/10.1073/pnas.96.24.13714
  55. Lange BM, Wildung MR, McCaskill D, Croteau R (1998) A family of transketolases that directs isoprenoid biosynthesis via a mevalonate-independent pathway. Proc Natl Acad Sci USA 95: 2100-2104 https://doi.org/10.1073/pnas.95.5.2100
  56. Lichtenthaler HK, Schwender J, Disch A, RohmerM (1997) Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathways. FEBS Lett 400: 271-274 https://doi.org/10.1016/S0014-5793(96)01404-4
  57. Lois LM, Campos N, RosaPutra S, Danielsen K, Rohmer M, Boronat A (1998) Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-l-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis. ProcNatl Acad Sci USA 95: 2105-2110 https://doi.org/10.1073/pnas.95.5.2105
  58. Lois LM, Rodriguez-Concepcion M, Gallego F, Campos N, Boronat A (2000) Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose synthase. Plant J 22: 503-513 https://doi.org/10.1046/j.1365-313x.2000.00764.x
  59. Lotan T, Hirschberg J (1995) Cloning and expression in Escherichia coil of the gene encoding $\beta$-c-4-oxygenase, that converts $\beta$-carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis. FEBS Lett 364: 125-128 https://doi.org/10.1016/0014-5793(95)00368-J
  60. Luttgen H, Rohdich F, Herz S, Wungsintaweekul J, HechtS, Schuhr CA, Fellermerier M, Sanger S, ZenkMH, Bacher A, Eisenreich W (2000) Biosynthesis of terpenoids: YchB protein of E. coli phosphorylates the 2-hydroxy group of 4-diphosphocytidyl-2Cmethyl-D-erythritol. Proc Natl Acad Sci USA 97: 1062-1067 https://doi.org/10.1073/pnas.97.3.1062
  61. Mandel MA, Feldmann KA, Herrera-Estrella L, Rocha-Sosa M, Leon P (1996) CLA1, a novel gene required for chloroplast development, is highly conserved in evolution. Plant J 9: 649-658 https://doi.org/10.1046/j.1365-313X.1996.9050649.x
  62. Mann V, Harker M, Peeker I, Hirschberg J (2000) Metabolic engineering of astaxanthin production in tobacco flowers. Nat Biotechnol 18: 888-892 https://doi.org/10.1038/78515
  63. Mann V, Peeker I, Hirschberg J (1994) Cloning and characterization of the gene for phytoene desaturase (Pds) from tomato (Lycopersicon esculentum). Plant Mol Biol 24: 429-434 https://doi.org/10.1007/BF00024111
  64. Marin E, Nussaume L, Quesada A, Gonneau M, Sotta B, Hugueney P, Frey A, Marion-Poll A (1996) Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisicacid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana. EMBO J 15: 2331-2342
  65. Misawa N, Mazamoto K, Hori T, Ohtani T, Boger P, Sandmann G (1994) Expression of an Erwinia phytoene desaturase gene not only confers multiple resistance to herbicides interfering with carotenid biosynthesis but also alters xanthophyll metabolism in transgenic plants. Plant J 6: 481-489 https://doi.org/10.1046/j.1365-313X.1994.6040481.x
  66. Misawa N, Nakagawa M, Kobayashi K, Yamano S, lzawa Y, Nakamura K, Harashima K (1990) Elucidation of the Erwinia uredouora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J Bacteriol 172: 6704-6712
  67. Misawa N, Shimada H (1998) Metabolic engineering for the production of carotenoids in non- carotenogenic bacteriaand yeasts. J Biotechnol 59: 169-181 https://doi.org/10.1016/S0168-1656(97)00154-5
  68. Niyogi KK, Grossman AR, Bjorkman O (1998) Arabidopsis mutants define a central role for the xanthophyll cycle in the regulation of phtosynthetic energy conversion. Plant Cell 10: 1121-1134 https://doi.org/10.1105/tpc.10.7.1121
  69. Pfander H (1992) Carotenoids: an overview. Meth Enzymol 213: 3-13 https://doi.org/10.1016/0076-6879(92)13105-7
  70. Peeker I, Chamovitz D, Linden H, Hirschberh J (1992) A single polypeptide catalyzing the conversion of phytoene to z-carotene is transcriptionally regulated during tomato fruit ripening. Proc Natl Acad Sci USA 89: 4962-4966 https://doi.org/10.1073/pnas.89.11.4962
  71. Peeker I, Gaboay R, Cunningham FX Jr, Hirschberh J (1996) Cloning and characterization of the cDNA for Iycopene betacyclase from tomato reveals decrease in its expression during fruit ripening. Plant Mol Biol 30: 807-819 https://doi.org/10.1007/BF00019013
  72. Ray J, Bird C, Maunders M, Grierson D, Schuch W (1987) Sequence of pTOM5, a ripening related cDNA from tomato. Nucleic Acids Res 15: 10587 https://doi.org/10.1093/nar/15.24.10587
  73. Ray J, Moureau P, Bird C, Bird A, Grierson D, Maunders M, Truesdale M, Bramley P, Schuch W (1992) Cloning and characterization of a gene involved in phytoene synthesis from tomato. Plant Mol Biol 19: 401-404 https://doi.org/10.1007/BF00023387
  74. Rodriguez-Concepcion M, Ahumada I, Diez-Juez E, Sauret-Gueto S. Lois LM, Gallego F, Carretero-Paulet L, Campos N, Boronat A (2001) 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening. Plant J 27: 213-222 https://doi.org/10.1046/j.1365-313x.2001.01089.x
  75. Rohdich F, Eisenreich W, Wungsintaweekul J, Richtrer G, Schuhr CA, Hecht S, Zenk MH, Bacher A (2000a) Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthesis of Arabidopsis thailana. Proc Natl Acad Sci USA 97: 6451-6456 https://doi.org/10.1073/pnas.97.12.6451
  76. Rohdich F, Kis K, Bacher, A. Eisenreich W (2001) The non-mevalonate pathway of isoprenoids: genes, enzymes, and intermediates. Curr Opin Chem Biol 5: 535-540 https://doi.org/10.1016/S1367-5931(00)00240-4
  77. Rohdich F, Wungsintaweekul J, Fellermeier M, Sagner S, Herz S, Kis K, Eisenreich W, Bacher A, Zenk MH (1999) Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl2C-methyJerythritol. Proc Natl Acad Sci USA 96: 11758-11763 https://doi.org/10.1073/pnas.96.21.11758
  78. Rohdich F, Wungsintaweekul J, Lutgen H, FischerM, Eisenreich W, Schuhr CA, Fellermeier M, Schramek N, Zenk MH, Bacher A (2000b) Biosynthesis of terpenoids: 4-diphosphocytidyl-2-Cmethyl-erythritol kinase from tomato. Proc Natl Acad Sci USA 97: 8251-8256 https://doi.org/10.1073/pnas.140209197
  79. Rohmer M, Knani M, Simonin P, Sutter B, Sahm H (1993) Isoprenoid biosynthesis in bacteria: a novel pathway for early steps leading to isopentenyl diphosphate. Biochem J 295: 517-524
  80. Romer S, Fraser PD, Kiano JW, Shipton CA, Mizawa N, Schuch W, Bramley PM, Hugueney P, Bouvier F, Camara B, Kuntz M(2000) Elevation of the provitamin A content of Transgenic tomato plants. Nat Biotechnol 18: 666-669 https://doi.org/10.1038/76523
  81. Romer S, Hugueney P, Bouvier F, Camara B, Kuntz M (1993) Expression of the genes encoding the early carotenoid biosynthetic enzymes in Capsicum annuum. Biochem Biophys Res Commun 196: 1414-1421 https://doi.org/10.1006/bbrc.1993.2410
  82. Ronen G, Carmel-Goren L, Zamir D, Hirschberg J (2000) An alternative pathway to B-formation in plant chromoplasts discovered by map-based cloning of beta and old-gold color mutations in tomato. ProcNatl Acad Sci USA 97: 11102-11107 https://doi.org/10.1073/pnas.190177497
  83. Ronen G, Cohen M, Zamir D, Hirschberg J (1999) Regulation of carotenoid biosynthesis during tomato fruit development: expression gene for lycopene epsilon-cyclase is down-regulated during ripening and is elevatedin the mutantdelta. Plant J 17: 341-351 https://doi.org/10.1046/j.1365-313X.1999.00381.x
  84. Rosati C, Aquilani R, Dharmapuri S, Pallara P, Marusic C, Tavazza R, Bouvier F, Camara B, Giulano G (2000) Metabolic engineering of beta-carotene and lycopene content in tomato fruit. Plant J 24: 413-420 https://doi.org/10.1046/j.1365-313x.2000.00880.x
  85. Sandmann G (1994) Carotenoid biosynthesis in microorganisms and plants. EurJ Biochem 223: 7-24 https://doi.org/10.1111/j.1432-1033.1994.tb18961.x
  86. Schledz M, AI-Babili S, von Uting J, Haudruck H, Rabbani S, Kleing H,Beyer P (1996) Phytoene synthase from Narcissus pseudonarcissus: functional expression, galactolipid requirement, topological distribution in chromoplasts and induction during flowering. Plant J 10: 781-792 https://doi.org/10.1046/j.1365-313X.1996.10050781.x
  87. Schwender J, Muller C, Zeidler J, Lichtenthaler HK (1999) Cloning and heterologous expression of a cDNA encoding 1-deoxy-Dxylulose-5-phosphate reductoisomerase of Arabidopsis thailana. FEBS Lett 455: 140-144 https://doi.org/10.1016/S0014-5793(99)00849-2
  88. Seo M, Koshiba T (2002) Complex regulation of ABA biosynthesis in plants. Trends Plant Sci 7: 41-48 https://doi.org/10.1016/S1360-1385(01)02187-2
  89. Shewmaker CK, Sheehy JA, Daley M, Colbum S, De DY (1999) Seed specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects. Plant J 20: 401-412 https://doi.org/10.1046/j.1365-313x.1999.00611.x
  90. Spurgeon SL, Porter JW (1980) Biosynthesis of carotenoids. In: Porter JW, Spurgeon SL, (eds), Biochemistry of Isoprenoid Compounds, John Wiley, New York, pp 1-122
  91. Sun Z, Gantt E, Cunningham FXJr (1996) Cloning and functional analysis of the beta-carotene hydroxylase of Arabidopsis thaliana. J Bioi Chem 271: 24349-24352 https://doi.org/10.1074/jbc.271.40.24349
  92. Takaya A, Zhang YW, Asawatreratanakul K, Wititsuwannakul D, Wititsuwannakul R, Takahashi S, Koyama T (2003) Cloning, expression and characterization of a functional cDNA clone encoding geranylgeranyl diphosphate synthase of Hevea brasiliensis. Biochim Biophys Acta 1625: 214-220 https://doi.org/10.1016/S0167-4781(02)00602-4
  93. Veau B, Courtois M, Oudin A, Chenieux JC, Rideau M, Clastre M (2000) Cloning and expression of cDNAs encoding two enzymes of the MEP pathway in Catharanthus roseus. Biochim Biophys Acta 1517: 159-163 https://doi.org/10.1016/S0167-4781(00)00240-2
  94. Walter MH, Hans J, Strack D (2002) Two distinctly related genes encoding 1-deoxy-D-xylulose 5-phosphate synthase: differential regulation in shoots and apocarotenoid-accumulating mycorrhizal roots. Plant J 31: 243-254 https://doi.org/10.1046/j.1365-313X.2002.01352.x
  95. Ye X, Al Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A ($\beta$-carotene) biosynthetic pathway. Science 287: 303-305 https://doi.org/10.1126/science.287.5451.303
  96. Zhu XF, Suzuki K, Okada K, Tanaka K, Nakagawa T, Kawamukai M, Matsuda K (1997a) Cloning and functional expression of a novel geranylgeranyl pyrophosphate synthase gene from Arabidopsis thaliana in Escherichia coli. Plant Cell Physiol 38: 357-361 https://doi.org/10.1093/oxfordjournals.pcp.a029174
  97. Zhu XF, Suzuki K, Saito T, Okada K, Tanaka K, Nakagawa T, Matsuda H, Kawamukai M (1997b) Geranylgeranyl pyrophosphate synthase encoded by the newly isolated gene GGPS6 from Arabidopsis thaliana is localized in mitochondria. Plant Mol Biol 35: 331-341 https://doi.org/10.1023/A:1005898805326

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