Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Light, Temperature, and Sucrose on Plant Regeneration from the Flower Organ Explant in Iris ensata

꽃창포 화기조직 절편체 배양으로부터 식물체 분화에 미치는 광.온도.당의 영향

  • Yoon, In-Kyung (Department of Life Resources, Catholic University of Daegu) ;
  • Koh, Jae-Chul (Department of Life Resources, Catholic University of Daegu)
  • 윤인경 (대구가톨릭대학교 생명자연학부) ;
  • 고재철 (대구가톨릭대학교 생명자연학부)
  • Published : 2003.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

A study was under taken to investigate the appropriate explant sources of flower organ and suitable cultural conditions such as light, temperature, and sucrose in plant regeneration of Iris ensata culture. Explants of perianth, ovary, pedicel, and peduncle of Iris ensata were cultured at different daylength (0, 8, 16, 24 hour), different temperatures (10, 15, 25, 3$0^{\circ}C$), and sucrose concentrations (1, 3, 6, 9%) on MS medium. Formation of adventitious roots from explants of Iris ensata was effective in the dark, while that of adventitous shoots was effective in the light. The optimum daylength for young plant regeneration was 16 hours. The optimum temperature for shoot formation of Iris ensata explants was $25^{\circ}C$ but the formation at 10 and 15$^{\circ}C$ was ineffective. Especi-ally, perianth and ovary was effective in shoot formation from flower organ expants. T-he optimum concentration of sucrose for shoots and roots formation of Iris ensata explants was 3 and 6%, respectively.

Iris 속 식물인 꽃창포의 번식 특성에서 종자의 잡종성과 적은 분얼로 인한 분주 번식의 어려움을 해결하고 고유한 품종의 특성을 유지하기 위해서는 조직배양을 통한 대량 번식의 구명이 필요하므로 Iris 속 식물의 화기부위 별 절편체를 이용하여 기내 번식에 의한 대량 증식방법을 정립하고자 본 실험을 수행하였다. 자생꽃창포의 화피기부조직, 자방, 소화경, 화경을 치상하여 기내배양환경에 적절한 환경을 구명하고자 조도 2000 Lux에서 일장 (0~24시간), 온도 (10~3$0^{\circ}C$), sucrose (l~9%)의 조건에서 배양하였다. 자생꽃창포의 화기 절편체로부터 유식물체 재분화에 적합한 일장은 16시간의 장일조건이, 온도 조건은 $25^{\circ}C$에서, sucrose 농도는 3%에서 가장 적합한 것으로 나타났으며 특히 화기 부위 중 화피기부조직과 자방 배양에서 높은 신초 형성을 보였다. 화기조직 치상절편체로부터 부정근의 분화는 암상태에서 촉진되어지고 sucrose는 6%에서 뿌리를 생장시키며 1$0^{\circ}C$, 15$^{\circ}C$의 저온에서는 신초,뿌리의 분화가 저조하였다.

Keywords

References

  1. Debergh PC, Maene LJ (1984) Pathological and physiological problems related to the in vitro culture of plants. Parasitica 40: 69-75
  2. Erwin J, Heins R, Carlson W, Biembaum J (1989) Do cool days/warm night work with plugs. Grower Talks 3: 46-49
  3. Han BH, Kim YJ, Choi JG (1994) Micropropagation of Alstroemeria spp. through rhizome tip culture. J Kor Soc Hort Sci 35: 172-177
  4. Hasegawa PM, Murashige T, Taketori FH (1973) Propagation of Asparagus through shoot apex culture. II. Ughtand temperature requirements, transplantability of plant and cytological characteristics. J Amer Soc Hort Sci 98: 143-148
  5. Hughes KW (1981) In vitro ecology: Exogenous factors affecting growth and morphogenesis in plant culture system. Environ Exper Botany 21: 281-288 https://doi.org/10.1016/0098-8472(81)90038-1
  6. Hussey G (1975) Totipotency in tissue explants and callus of some members of the Li-liaceae, lridaceal, and Amaryllidaceae. J Exp Bot 26: 253-262 https://doi.org/10.1093/jxb/26.2.253
  7. Ichihashi S, Kato S (1986) Clomal propagation of Iris kempeferi by means of flower organ culture. Bull Aichi Univ Education 35: 135-143
  8. Jehan H, Courtois D, Ehret C, Lerch K, Petiard V (1994) Plant regeneration of Iris pallida Lam. and Iris germanica L. via somatic embryogenesis from leaves, apices and young flowers. Plant Cell Rep 13:671-675
  9. Kozai T (1991a) Controlled environment in conventional and automated micropropagation In: Cell Culture and Somatic Cell Genetics of Plants, Vol. 8(ed. Vasil I), Academic Press. Inc. 213-230
  10. Kozai T (1991b) Autotrophic micropropagation, In: Biotechnology in culture and forestry, Vol. 17, High Tech and Micropropagation I (ed Bajaj, YPS). Springer Verlag. Bergin. 312-343
  11. Kohlein F (1981) IRIS. Timber Press, Inc.
  12. Meyer MM, Fuchigami LH, Roberts AN (1975) Propagation of tall bearded irises by tissue culture. Hort Sci 10:479-480
  13. Meyer MM, Milbrath GM (1977) In vitro propagation of horseradish with leaf pieces. Hort Sci 12: 544-545
  14. Murashige T, Skoog F (1962) A revised midium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Schnapp SR, Preece JE (1986) In virto growth reduction of tomato and carnation microplants. Plant Cell Tiss Org Cult 6: 3-8 https://doi.org/10.1007/BF00037752
  16. Simonse J, Hildebrandt AC (1971) In vitro growth and differentiation of Gladiolus plants from cultures. Can J Bot 49: 1817-1819 https://doi.org/10.1139/b71-256
  17. Tanaka F, Watanabe Y, Shimada N (1990) Effect of $O_2$ concen-tration on photorespiration Chrysathemum morifolium plantIets in plant tissue culture. Plant Tiss Cult Lett 7: 85-91 https://doi.org/10.5511/plantbiotechnology1984.7.85
  18. Watanabe K, Watanabe Y, Shimada N (1990) Effect of sucrose concertration in the medium on growth apparent photosynthesis and ribulose-1, 5-bisphosphate carboxylase of Spathyphyllum plantIes in aeration culture. Plant Tiss Cult Lett 7: 74-79 https://doi.org/10.5511/plantbiotechnology1984.7.74
  19. Yabuya T, Ikeda Y, Adachi T (1991) In vitro propagation of Japanese garden iris, Iris ensata Thunb. Euphytica 57: 77-81

Cited by

  1. Effect of plant growth regulators on plant regeneration from the Belamcanda chinensis vol.37, pp.3, 2010, https://doi.org/10.5010/JPB.2010.37.3.337