Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Hormone and Several Factors on the Regeneration and Transformation rate of Potato Cultivars Bred in Korea

국내 육성 감자의 재분화와 형질전환 효율에 미치는 생장조절제의 조성 및 몇 가지 요소

  • Yi, Jung-Yoon (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA) ;
  • Seo, Hyo-Won (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA) ;
  • Cho, Ji-Hong (Highland Crop Research Division, National Highland Agricultural Experiment Station, RDA) ;
  • Lee, Shin-Woo (Department fo Crops Biotechnology, Jinju National University) ;
  • Yun, Han-Dae (Division of Applied Life Science, Gyeongsang National University)
  • 이정윤 (농촌진흥청 고령지농업시험장) ;
  • 서효원 (농촌진흥청 고령지농업시험장) ;
  • 조지홍 (농촌진흥청 고령지농업시험장) ;
  • 이신우 (진주산업대 작물생명과학과) ;
  • 윤한대 (경상대학교 응용생명과학부)
  • Published : 2003.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The optimal condition of in vitro regeneration and transformation were investigated for newly bred potato varieties in Korea. Leaf and internodal stem tissues of 'Chubak', 'Namsuh', 'Jasim', 'Jopung' and 'Jowon' were used to investigate the influence of growth regulator on regeneration efficiency The effect of phenolic compound acetosyringone on gene transformation efficiency was also investigated. Leaf tissue of 'Jowon' and internodal stem tissues of 'Jopung' were showed high regeneration efficiency on M5 medium supplemented with 0.1 mg/L GA₃, 2.0 mg/L Zeatin and 0.01 mg/L NAA. The other three potato cultivars were showed low regeneration efficiency less than 25%. The effect of acetosyringone on Agrobacterium-mediated gene transformation with leaf and internodal stem tissues were noticeable. By adding the 75 μM of acetosyringone during the Agrobacterium innoculation, the transformation efficiency was increased up to 1.5∼4.0 fold compare to non-treated control. In case of 'Jowon' the transformation efficiency was 87.9% in leaf tissue and 'Jopung' was 68.4% in internodal stem tissues.

국내에서 육종된 감자 품종들에 대하여 재분화 및 형질전환효율에 영향을 미치는 여러 요인들을 조사하고 품종별 최적 조건을 규명하고자 하였다. 최근 육종된 5품종 '추백', '남서', '자심', '조풍'과 '조원'의 잎과 줄기 조직을 재료로 사용하여 배지 내 호르몬 조성별 신초형성률을 조사하였다. 신초형성률은 잎 조직의 경우 '조원'이 GA₃0.1 mg/L, Zeatin 2.0mg/L과 NAA 0.01 mg/L가 포함된 M5 배지에서 90%이상으로 가장 높은 반면 다른 공시품종은 20% 미만이었으며 줄기조직을 치상한 경우 '조풍'이 M5 배지에서 70%의 신초형성률을 나타내었으나. 다른 품종은 25% 이하의 신초형성률을 나타내어 품종별, 조직별로 큰 차이를 나타내었다. 감자의 형질전환 시 Agrobacterium 접종과정에서 75 μM의 acetosyringone을 처리한 경우 잎 조직은 '조원'이 87.9%, 줄기 조직은 '조풍'이 68.4%로 높은 형질전환효율을 나타내어 acetosyringone을 처리하지 않은 대조구에 비하여 1.5∼4.0배의 높은 형질전환효율을 나타냈다.

Keywords

References

  1. An G. Ebert PR, Mitra A, Ha SB (1988) Binary Vectors. In: Gelvin SB, Schilperoort RA, Verma DPS, Plant Molecular Biology Manual. Kluwer Academic Publisher, Boston, PMAN-A3: 1-19
  2. Binding H, Nehls R, Schieder O, Sopory SK, Wenzel G (1978) Regeneration of mesophyll protoplasts isolated from dihaploid clones of Solanum tuberosum. Physiol Plant 43: 52-54 https://doi.org/10.1111/j.1399-3054.1978.tb01565.x
  3. Bradshaw JE, Mackey GR (1994) Potato Genetics. CAB International. Wallingford
  4. Bragod-Aas M(1977) Regeneration of plants from callus of potato tubers. Acta Hort 78: 133-137
  5. Carputo D, Cardi T, Chiari T, Ferraiolo G, Frusciante L (1995) Tissue culture response in various wild and cultivated Solanum germplasm accession for exploitation in potato breeding, Plant Cell Tiss Org Cult 41: 151-158 https://doi.org/10.1007/BF00051584
  6. Chen CY, Winans SC (1991) Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by the Escherichia colilac promoter. J Bacteriol 173: 1139-1144
  7. Choi KH, Jeon JH, Kim HS, Joung YH, Yang DC, Joung H (1996) An effective method for the selection of transgenic potato using mouse adenosine deaminasegene. Korean J Plant tissue culture 23:97-101
  8. D'Amato F (1975) The problem of genetic stability in plant tissue and cell cultures. Crop Genetic Resources for Today and Tomorrow. Cambridge Univ Press. pp 333-348
  9. De Block M (1988) Genotype-independent leaf disc transformantion of potato (Solanum tuberosum) using Agrobacterium tumefaciens. Theor Appl Genet 76: 767-774 https://doi.org/10.1007/BF00303524
  10. Dobigny A, Tizroutine S, Gaisne C, Haicour R, Rossignol L, Ducreux G, Sihachakr D (1996) Direct regeneration of transformed plants from stem fragment of potato inoculated with Agrobacteium rhizogenes. Plant Cell Tiss Org Cult 45: 115-121 https://doi.org/10.1007/BF00048753
  11. Dobigny A, Ambroise A, Haicour R, David C, Rossignol L, Sihachakr D (1995) Transformation of potato using mannopine and cucumopine strains of Agrobacterium rhizogenes. Plant Cell Tiss Org Cult 40: 225-230 https://doi.org/10.1007/BF00048127
  12. Engstrom P, Zambryski P, Van Montague M, Stachel S (1987) Characterization of Agrobacterium tumefaciens virulence proteind induced by the plant factor acetosyrongone. J Mol BioI 197: 635-645 https://doi.org/10.1016/0022-2836(87)90470-0
  13. Ghislain M, Querci M, Bonierbale M, Golmirzaie R, Nels R (1997) Biotechnology and the potato. CIP, Peru. Lima
  14. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativaL.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6: 271-282 https://doi.org/10.1046/j.1365-313X.1994.6020271.x
  15. Horsch RB, Fry JE, Hoffmann NL, Wallroth M, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 27: 1229-1231
  16. Ishida Y, Saito H, Ohta S, Hiei Y, Komarl T, Kumashiro T (1996) High efficiency transformation of maize mediated by Agrobacterium tumefaciens. Nature Biotech 14:745-750 https://doi.org/10.1038/nbt0696-745
  17. Joung YH, Jeon JH, Choi KH, Kim HS, Joung H (1996) Transformation of potato leafroll virus coat protein gene into potato. Korean J Plant Tiss Cult 23: 77-81
  18. Mathews H, Bharathan N, Litz RB, Narayanan KR, Rao PS, Bhatia CR (1990) The promotion of Agrobacterium mediated transformation in Atropa belladona L. by acetosyringone. J Plant Physiol 136: 404-409 https://doi.org/10.1016/S0176-1617(11)80027-1
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  20. Ooms G, Burrell MM, Karp A, Bevan M, Hille J (1987) Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium. Theor Appl Genet 73: 744-750
  21. Ross H (1958) Virus resistance of the potato. European Potato J 1: 1-19
  22. Sheerman S, Bevan MW (1988) A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors. Plant Cell Rep 7: 13-16 https://doi.org/10.1007/BF00272967
  23. Shepard JF, Totten RE (l977) Mesophyll cell protoplasts of potato. Isolation, proliferation, and plant regeneration. Plant Physiol 60: 313-316 https://doi.org/10.1104/pp.60.2.313
  24. Sheikholeslam SN, Weeks DP (1987) Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Mol BioI 8: 291-298 https://doi.org/10.1007/BF00021308
  25. Shimoda N, Yamamoto T, Nagamine J, Usami S, Katayama M, Sakagami Y, Machida Y (1990) Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides. Proc Natl Acad Sci USA 87: 6684-6688 https://doi.org/10.1073/pnas.87.17.6684
  26. Stiekema WJ, Heidekamp F, Louwerse JD, Verhoeven HA, Dijkhuis P (1988) Introduction of foreign genes into potato cultivars Bintje and Desiree using an Agrobacterium tumefaciens binary vector. Plant Cell Rep 7: 47-50 https://doi.org/10.1007/BF00272976
  27. Sung SK, Choi SB, Jeon, JS, Park MC, Lee KW (1994) Expression patterns of CaMV 35S promoter-GUS in transgenic potatoes and their clonal progenies. J Plant BioI 37: 17-25
  28. Teresa MF, Julapark C, Tanksley SD (l995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol BioI Rep 13: 207-209 https://doi.org/10.1007/BF02670897
  29. Visser RGF, Jacobsen E, Hesseling-Meinders A, Schans MJ, Witholt B, Feenstra WJ (1989) Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments. Plant Mol Bioi 12: 329-337 https://doi.org/10.1007/BF00043210
  30. Yuom JW, Jeon JH, Jung JY, Lee BC, Kang WJ, Kim MS, Kim CJ, Joung H, Kim HS (2002) Introduction of VP6 gene into potato plant by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes. Korean J Plant Biotech 29: 93-98 https://doi.org/10.5010/JPB.2002.29.2.093

Cited by

  1. Recent advances in the applications of tissue culture and genetic transformation in potato vol.37, pp.4, 2010, https://doi.org/10.5010/JPB.2010.37.4.456
  2. An efficient transformation method for a potato (Solanum tuberosum L. var. Atlantic) vol.42, pp.2, 2015, https://doi.org/10.5010/JPB.2015.42.2.77