Antimicrobial and Antitumoral Activities of Candida kefyr TEP 7 Isolated from Tibetan Fermented Milk

티벳산 발효유로부터 분리한 Candida kefyr TFP 7의 항균활성 및 항암활성

  • 윤원호 (서일대학 식품가공과) ;
  • 나영미 (건국대학교 축산가공학과) ;
  • 김창한 (건국대학교 축산가공학과)
  • Published : 2003.03.01

Abstract

This study was carried out to investigate antimicrobial and antitumoral activities of Candida kefyr TFP7 isolated from Tibetan fermented milk Strains of TFP1∼10 were isolated from Tibetan fermented milk by agar diffusion method using potato dextrose agar(PDA). Antimicrobial activities were examined against 18 microorganisms of food-related bacteria, yeast, algae, fungi and actinomycetes isolated from soil. Antitumor activities were examined against 9 human tumor cell lines. Strains of TFP2∼10 showed strong antimicrobial activities against Micrococcus luteus ATCC l1880, and strains of TFP6∼10 to actinomycetes, Streptomyces murinus JCM 4333. In antitumor test, all isolated strains(TEP1∼10) showed the growth inhibition of SNU-5 and SW-534 by 60% and 70%, respectively. Among those, the strain TFP7 showed the most antitumor activity, which was 77.5% for SNU-5 and 76.5% for SW-534. The strain was identified as Candida kefyr by use of API 20C AUX kit and scanning electron micrograph.

티벳산 발효유로부터 분리한 Candida kefyr TFP7의 항균활성 및 항암활성을 검토하기 위하여 PDA배지를 이용한 십진 희석법에 의해 10균주(TFP1∼10)를 분리하여 몇 종의 병원균과 식중독균, 식품과 관련이 있는 세균, 효모, 곰팡이, 녹조류 및 토양으로부터 분리한 방선균 등 18균주에 대하여 항균활성을 조사하였으며, 또한, 9종류의 인체 암세포주에 대하여 항암활성을 비교하였다. 항균활성 검사에서는 Gram 양성균 중 iwicrococcus iuteus ATCC l1880에 대하여 분리균주 (TFP2∼10)와 방선균인 Styeptomyces murinus JCM 4333에 패하여 분리균주(TFP6∼10)가 뚜렷한 생육억제효과를 나타내었다. 한편, 항암활성 검사에서는 SNU-5(human stomach carcinoma), SW-534(human larynx carcinoma)에 대하여 분리 균주(TFP1∼10) 모두 각각 60%, 70%이상의 생육억제 효과를 나타내었다. 특히, 균주 TFP7은 SNU-5, SW-534에 대하여 각각 77.5%, 76.5%의 가장 우수한 생육억제 효과를 나타내었다.

Keywords

References

  1. Allcroft, R. and Camaghan, R. B. A. (1962) Groundnut toxicity : An examination tbr toxin in human products from animals ted toxic groundnut meal. Vet. Rec. 75, 259-263
  2. Alley, M. C., Scudiero, D. A., Monks, A., Hursley, M. L.,Czerwinski, M. J., Fine, D. L., Abbott, B. J., Mayo, J. G., Shoemaker, R. H., and Boyd, M. R. (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research 48, 589-601
  3. Bottazzi, V. (1980) A note on scanning electron microscopy of micro-organisms associated with the kefir granule. J. Applivd Bacteriolom 48, 265-268 https://doi.org/10.1111/j.1365-2672.1980.tb01225.x
  4. Choi, G. H. and Kim, C. H. (2002) Growth inhibition of extracts from sulfur feed duck meat against various tumor cell lines. Kor. J. Food Sci. Ani. Resour. 22, 348-351
  5. Ham, J. S., Jeong, J. Y., Jeong, S. G., Ahn, J. N., Ahn, Y. T., K-im, S. K... and Kim, H. V. (1999) Effects of Feeding with Lactobacillus ptantarum and Candida kefyr Isolated from Mongolian Koumiss on the Growth and Fecal Microflora of Broilers. Korean J. Dairy Sci. 21(3) : 241-246
  6. K.andler, 0. and Kunath, P. (1983) LactobaciUus kefir sp. nov., A component of the microflora of kefir. Systematic and Applied Microbiolovy 4, 286-294 https://doi.org/10.1016/S0723-2020(83)80057-5
  7. K.oneman, E. W., Aelen, S. D., Dowell, V. R., and Sommers, H. M. (1979) Color atlas and textbook of diagnostic microbiology. Lippincott Co., Philadelphia. Toronto, pp. 316-349
  8. K.oroleva, N. S. and Bavina, N. A. (1970) Effects of conditions of kefir fungus cultivation on the microflora and biochemical characteristics of kefir starters. Proc. 8th Int. Dairy Cong 1E., 413
  9. Lee, K. H. (2000) Studies on antitumor, antileukemic and apoptosis induction activities of di(2-ethy1hexy1)Phtha1ate isolated from Aloe veru Linne. Ph. D. thesis. Konkuk Univ., Seoul, Korea
  10. Na, Y. M. (2003) Antimicrobial activity and Antitumor activity by Cundida kefyr TFP7 Isolated from Tibetan Fermented Milk. M. S. thesis, K.onkuk Univ., Seoul, Korea
  11. Nagaoka, M., Muto, M., Nomto, K.., Matuzaki, M., Watanabe, T., and Yokokura, T. (1990) Structure of Polysaccharide Peptidogly-can Complex from the Cell Wall of LactohacitIus casei Y[T 9018. J. Biochem. 108, 568-571 https://doi.org/10.1093/oxfordjournals.jbchem.a123243
  12. Nakanishi,T.(1967)牛乳と乳製品の微微生物.pp.157
  13. Suganuma, M., Ohkura, Y., Okabe, S., and Fujiki, H. (2001)Combination cancer chemoprevention with green tea extract and sulindac shown in intestinal tumor formation in Min mice. Journul of Cancer Research and Ctinical OncoIoyy 127, 69-72 https://doi.org/10.1007/s004320000189
  14. Wiseman, U. W. and Marth, E. H. (1983) Behavior of aflatoxin Ml in yogurt, butter milk and kefir. J. Food Protection 46(2), 115-118 https://doi.org/10.4315/0362-028X-46.2.115
  15. Yan, M., Lin, H., Shen, Y., and Wang, Q. (2001) Studies on inhibiting activities of five antitumour drugs to human cancer cell in vitro with MTT assay. Jourwl of Chinese Medicinal Materials24, 418-419
  16. Yang, S. J., Yoon, J. W., Seo, K-. S., K-oo, H. C., Kim. S. H., Bae, H. S., Baek, Y. and Park, Y. H. (1999) Prophylactic KfTects of Bifidobacterium Iongum HY8001 against Escherichia coli 0157 :117 and Satmonella typhiimurium DT104 Enteric Infection and Evaluation of Vero Cytotoxin Neutralizing Effccts. Kor. J. Appl. Microhiot. Biolechiwl. Vol. 27, No. 5, 419-425