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Effect of Ethanol on the Production of Cellulose and Acetic Acid by Gluconacetobacter persimmonensis KJ145

Gluconacetobacter persimmonensis KJ145를 이용한 Bacterial Cellulose 및 초산발효에 미치는 Ethanol의 영향

  • 이오석 (계명대학교 정통 미생물자원개발 및 산업화연구센터) ;
  • 장세영 (계명대학교 식품가공학과) ;
  • 정용진 (계명대학교 식품가공학과)
  • Published : 2003.03.01

Abstract

We investigated the effect of ethanol on the production of cellulose and acetic acid fermentation by Gluconacetobacter persimmonensis KJ145. Results showed that bacterial cellulose productivity was highest when 2% ethyl alcohol was added to apple-juice medium. For acetic acid production, 7% ethyl alcohol was needed. Optimal concentration of ethyl alcohol was 5% for simultaneous production of bacterial cellulose and acetic acid. For simultaneous production of bacterial cellulose and acetic acid, optimal nitrogen source and optimal concentration were corn steep liquor and 15% (w/v), respectively Optimal culture time for simultaneous production of bacterial cellulose and acetic acid was 14 days. At the optimal condition, Cluconacetobacter persimmonenis KJ145 produced 7.55 g/L of bacterial cellulose (dry weight).

G. persimonensis-KJ145를 차용하여 BC 생산과 식초생산에 미치는 에탄올의 영향을 조사하였다. 그 결과 사과쥬스배지에 에탄올을 2%(v/v) 첨가하였을 때 BC의 생산성이 가장 좋았으며, 7%(v/v)를 첨가하였을 때 총산의 함량이 가장 높았다. BC와 식초를 동시 생산하기 위해서는 에탄올을 5%(v/v) 첨가하는 것이 가장 좋을 것으로 생각되며 발효과정 중의 식초 품질 변화에 대한 보다 자세한 연구가 요구되었다. BC와 식초 생산에 가장 효과적인 질소원은 CSL인 것으로 조사되었고 최적 농도는 15%(w/v)이었다. 배양시간의 영향을 조사한 결과 14일간 배양하는 것이 가장 많은 양의 BC를 생산하였으며, 최적조건에서 생산되는 BC 생산량은 7.55 g/L이었다.

Keywords

References

  1. 식품의약청. 2000. 식품공전. 한국식품공업협회. p 382.
  2. Jeong YJ, Lee MH. 2000. A view and prospect of vinegar industry. Food Industry and Nutrion 5: 7-12.
  3. Lee OS, Jeong YJ. 2001. Industrial application and biosynthesis of bacterial cellulose. Food Industry and Nutrition 6: 10-14.
  4. Brown AJ. 1886. On an acetic ferment which forms cellulose. J Chem Soc 49: 172-186. https://doi.org/10.1039/ct8864900172
  5. Brown AJ. 1886. An acetic ferment which forms cellulose. J Chem Soc 49: 432-439. https://doi.org/10.1039/ct8864900432
  6. Jonas R, Farah LF. 1998. Production and application of microbial cellulose. Polym Degrad Stab 59: 101-106. https://doi.org/10.1016/S0141-3910(97)00197-3
  7. Vandamme EJ, Baets SD, Vanbaelen A, Joris K, Wulf PD. 1998. Improved production of bacterial cellulose and its application potential. Polym Degrad Stab 59: 93-99. https://doi.org/10.1016/S0141-3910(97)00185-7
  8. Klemm D, Schumann D, Udhardt U, Marsch S. 2001. Bacterial synthesized cellulose-artificial blood vessels for microsurgery. Prog Polym Sci 26: 1561-1603 https://doi.org/10.1016/S0079-6700(01)00021-1
  9. Jeong YJ, Lee IS. 2000. A view of utilizing cellulose produced by Acetobacter bacteria. Food Industry and Nutrition 5: 22-29.
  10. Lee OS, Jang SY, Jeong YJ. 2002. Culture condition for the production of bacterial cellulose with Gluconacetobacter persimmonensis KJ145. J Korean Soc Food Sci Nutr 31: 572- 577. https://doi.org/10.3746/jkfn.2002.31.4.572
  11. Hestrin S, Schramm M. 1954. Synthesis of cellulose by Acetobacter xylinum: 1. Micro-method for the determination of cellulose. Biochem 58: 345-352.
  12. Naritomi T, Kouda T, Yano H, Yoshinaga F. 1998. Effect of ethanol on bacterial cellulose production from fructose in continuous culture. J Fermen Bioeng 85: 598-603. https://doi.org/10.1016/S0922-338X(98)80012-3
  13. Lee HC, Zhao X. 1996. The optimal medium composition for the production of microbial cellulose by Acetobacter xylinum. Kor J Biotechnol Bioeng 11: 550-556.
  14. Son HJ, Heo MS, Kim YG, Lee SJ. 2001. Optimization of fermentation conditions for the production of bacterial cellulose by a newly isolated Acetobacter sp. A9 in shaking cultures. Biotechnology and Applied Biochemistry 33: 1-5 https://doi.org/10.1042/BA20000065
  15. Ishikawa A, Matuoka M, Tsuchida T, Yoshinaga F. 1995. Increase in cellulose production by sulfaguanidine-resistant mutants derived from Acetobacter xylinum subsp. sucrofermentans. Biosci Biotech Biochem 59: 2259-2262. https://doi.org/10.1271/bbb.59.2259
  16. Matsuoka M, Tsuchida T, Matushita K, Adachi O, Yshinaga F. 1996. A synthetic medium for bacterial cellulose production by Acetobacter xylinum subsp. sucrofermentans. Biosci Biotech Biochem 60: 575-579. https://doi.org/10.1271/bbb.60.575

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