KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Chromatographic Characterization of Pentoxifylline and a Mixture of Caffeine-theophylline Imprinted Polymer

Pentoxifylline과 Caffeine-theophylline Imprinted Polymers의 크로마토그래피 특성

  • 홍승표 (인하대학교 공과대학 화학공학과) ;
  • 왕덕선 (인하대학교 공과대학 화학공학과) ;
  • 구윤모 (초정밀분리기술센터, 인하대학교) ;
  • 노경호 (인하대학교 공과대학 화학공학과)
  • Published : 2003.02.01
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Abstract

Two molecular imprinted polymers (MIPs) were prepared by using pentoxifylline and a mixture of caffeine-theophylline (2:1, mole ratio) as the templates, MAA as the monomer, and EDMA as the crosslinker The obtained polymer particles (particle size 25-35 $\mu\textrm{m}$) were packed into a HPLC column (150$\times$3.9mm). The selectivity and chromatographic characterization of the imprinted polymers were studied using pentoxifylline, caffeine, theophylline, theobromine as samples and acetonitrile as the mobile phase, Both imprinted polymers showed increased affinity for structural analogues. By using a mixture of caffeine-theophylline templates MIPs, good separations of caffeine, theobromine and theophylline were obtained, and pentoxifylline and caffeine were partly separated.

Pentoxifylline MIPs에서 시료와 기능성 단위체 사이에는 수소결합이 형성되었다. 수소결합의 능력은 분자구조상 질소원자의 위치에 의해 결정되어졌다. Pentoxifylline과 caffeine theophylline MIPs 모두는 구조상 유사한 시료들과 affinity가 증가하는 결과를 얻어냈다. 특히 caffiene-theophylline MIPs에서 높은 수치의 체류인자와 IF를 얻었다. 이러한 결과로 적합한 복합 주형분자를 사용할 때 우리가 원하는 목적시료만을 선택적으로 분리 할 수 있고 그러므로 solid phase extraction에 사용되기에 적합하다는 결과를 얻어냈다.

Keywords

References

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